Proteomics of desiccation tolerance during development and germination of maize embryos

Huang, Hui Yu; Møller, Ian Max; Song, Song-Quan

doi:10.1016/j.jprot.2011.10.036

Cited by 99 publications

(105 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Wang et al (2012) also observed that groups of seeds resistant to desiccation presented higher accumulation of heat-stable proteins. According to Huang et al (2012), desiccation tolerance loss in maize seeds is also associated with a reduction in the content of hydrophilic proteins during germination. In a study with sunflowers, Personat et al (2014) verified that the simultaneous overexpression of two genes that transcribes heat-stable protein, increased the tolerance to desiccation, reaching to drastic levels of dehydration.…”

Section: Heat-stable Proteinsmentioning

confidence: 99%

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

2017

View full text Add to dashboard Cite

During germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins.Keywords: desiccation tolerance, forest seeds, drying.Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação ResumoDurante a germinação, sementes ortodoxas tornam-se gradualmente intolerantes à dessecação, e por isso podem ser utilizadas como modelo para o estudo da recalcitrância. No presente trabalho realizou-se uma caracterização dos aspectos fisiológicos, bioquímicos e ultraestruturais da perda da tolerância à dessecação de sementes de Anadenanthera colubrina em processo germinativo. Para isso as sementes foram embebidas durante 0 (controle), 2,8,12 e aproximadamente 18 horas (sementes germinadas com 1 mm de radícula), secas por 72 horas, reidratadas e a sobrevivência avaliada. Ao longo da embebição foram realizadas análises citométricas, ultraestruturais e extração de proteínas resistentes ao calor e após embebição e secagem foram avaliados danos ultraestruturais. A tolerância à dessecação foi totalmente perdida após a protrusão radicular. Não houve aumento do conteúdo de DNA 4C quando a tolerância à dessecação foi perdida. Características ultraestruturais de células de radículas de 1 mm assemelharam-se às encontradas em sementes recalcitrantes tanto no estado hidratado quanto desidratado. A perda da tolerância à dessecação coincidiu com a redução do conteúdo de proteínas resistentes ao calor.Palavras-chave: tolerância à dessecação, sementes floretais, secagem.

show abstract

Section: Heat-stable Proteinsmentioning

confidence: 99%

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

2017

View full text Add to dashboard Cite

show abstract

“…The up-regulated expression of chaperone genes demonstrates that E. pusillum can likely cope with problems associated with protein misfolding and aggregation induced by desiccation through up-regulating its main chaperones. In addition, the increasing expression of proteasome implies that the misfolded proteins were likely quickly degraded [61].…”

Section: The Common Drought-resistant Mechanisms In E Pusillummentioning

confidence: 99%

Comparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms

Wang

Zhang

Zhou

et al. 2014

Sci. China Life Sci.

View full text Add to dashboard Cite

The lichen-forming fungus was isolated from the desert lichen Endocarpon pusillum that is extremely drought resistant. To understand the molecular mechanisms of drought resistance in the fungus, we employed RNA-seq and quantitative real-time PCR to compare and characterize the differentially expressed genes in pure culture at two different water levels and with that in desiccated lichen. The comparative transcriptome analysis indicated that a total of 1781 genes were differentially expressed between samples cultured under normal and PEG-induced drought stress conditions. Similar to those in drought resistance plants and non-lichenized fungi, the common drought-resistant mechanisms were differentially expressed in E. pusillum. However, the expression change of genes involved in osmotic regulation in E. pusillum is different, which might be the evidence for the feature of drought adaptation. Interestingly, different from other organisms, some genes involved in drought adaption mechanisms showed significantly different expression patterns between the presence and absence of drought stress in E. pusillum. The expression of 23 candidate stress responsive genes was further confirmed by quantitative real-time PCR using dehydrated E. pusillum lichen thalli. This study provides a valuable resource for future research on lichen-forming fungi and shall facilitate future functional studies of the specific genes related to drought resistance. lichen, mycobiont, dehydration, drought adaption, drought resistant Citation:Wang YY, Zhang XY, Zhou QM, Zhang XL, Wei JC. Comparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms.

show abstract

“…In this stage, most of the seeds of different cultivars have high moisture content, which requires artificial drying, a process that may reduce seed quality, resulting in low germination and vigor. Nevertheless, this reduction depends on the expression of genes associated with the trait of tolerance to high drying temperatures, which varies according to the genotype (Huang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds

Andrade

Pinho²,

Pinho³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We quantified and characterized the expression of heatresistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two nontolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they were then subjected to tests of moisture content, germination, first count of germination, accelerated aging, and cold test. The seeds were stored in a freezer for later analysis of expression of heat-resistant proteins by means of real-time PCR, electrophoresis, and spectrophotometry. We observed that heat-resistant proteins are expressed in a differential manner in seeds from different lines and at different stages of development. The expression of heatresistant proteins was earlier in lines tolerant to high drying temperatures. Greater germination and vigor values was found for seeds collected at the last stage of development.

show abstract

Proteomics of desiccation tolerance during development and germination of maize embryos

Cited by 99 publications

References 73 publications

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

Comparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms

Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds

Contact Info

Product

Resources

About