Proteomics of corynebacteria: From biotechnology workhorses to pathogens

Abstract

Corynebacteria belong to the high G+C Gram-positive bacteria (Actinobacteria) and are closely related to Mycobacterium and Nocardia species. The best investigated member of this group of almost seventy species is Corynebacterium glutamicum, a soil bacterium isolated in 1957, which is used for the industrial production of more than two million tons of amino acids per year. This review focuses on the technical advances made in proteomics approaches during the last years and summarizes applications of these techniques with respect to C. glutamicum metabolic pathways and stress response. Additionally, selected proteome applications for other biotechnologically important or pathogenic corynebacteria are described.

Proteome-wide selected reaction monitoring assays for the human pathogen Streptococcus pyogenes

“…During an infection, pathogenic bacteria can rapidly alter their proteome composition to adapt to hostile environments and evade immune response1234. How the bacteria regulate their proteome composition in vivo to accomplish host environment adaption and immune response evasion is, however, still unclear.…”

Global relative and absolute quantitation in microbial proteomics

“…Considerable efforts in proteomic research are undertaken for pathogenic bacteria [21][22][23]. As the emergence and propagation of multiresistant strains, for example, of S. aureus, are a major threat in our health systems today [24], there is a strong need for novel antibiotic strategies.…”

The Genus Corynebacterium in the Genomic Era

“…This species is investigated in relation to its genetics and physiology and, consequently, a diversity of information about its molecular biology and biochemistry available, including a variety of proteomic techniques. In consequence of the membrane organization with high concentration of mycolic acids, C. glutamicum has been used as a model for development and new proteomic technologies [70].…”