Proteomics of bronchoalveolar lavage fluid

Wattiez, Ruddy; Falmagne, Paul

doi:10.1016/j.jchromb.2004.10.029

Cited by 128 publications

(116 citation statements)

References 72 publications

(96 reference statements)

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“…More recently, we have demonstrated 16 O/ 18 O labeling combined with the AMT tag strategy as an effective global quantitative approach for quantifying relative protein abundance differences in human plasma (31). By incubating tryptic peptides in 18 O water (55,94) in the presence of trypsin, the 18 O atoms are incorporated into the carboxyl terminus of tryptically cleaved peptides via a postdigestion trypsin-catalyzed oxygen exchange reaction.…”

Section: Strategiesmentioning

confidence: 99%

“…By incubating tryptic peptides in 18 O water (55,94) in the presence of trypsin, the 18 O atoms are incorporated into the carboxyl terminus of tryptically cleaved peptides via a postdigestion trypsin-catalyzed oxygen exchange reaction. The 16 O/ 18 O-labeled peptide pairs provide a 4-Da mass difference (Fig. 7A), which allows a high resolution mass spectrometer such as FTICR or TOF to effectively resolve the 16 O-and 18 O-labeled peptide pairs and accurately measure the relative abundances.…”

Section: Strategiesmentioning

confidence: 99%

“…As a result, analysis of various other biofluids/tissues has gained increasing attention. Due to their proximity to the source of disease or perturbation in the body, tissues (14) and various biofluids such as cerebrospinal fluid (15), bronchoalveolar lavage fluid (16), synovial fluid (17), nipple aspirate fluid (18), saliva (19), and urine (20) are believed to provide a more focused pool of potential biomarkers of interest. In addition, tumor interstitial fluids have also been reported as a novel source for proteomics biomarker and therapeutic target discovery (21), offering a promising alternative to direct tissue analysis.…”

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confidence: 99%

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Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications

Qian

Jacobs

Liu

et al. 2006

Molecular & Cellular Proteomics

327

284

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Recent advances in proteomics technologies provide tremendous opportunities for biomarker-related clinical applications; however, the distinctive characteristics of human biofluids such as the high dynamic range in protein abundances and extreme complexity of the proteomes present tremendous challenges. In this review we summarize recent advances in LC-MS-based proteomics profiling and its applications in clinical proteomics as well as discuss the major challenges associated with implementing these technologies for more effective candidate biomarker discovery. Developments in immunoaffinity depletion and various fractionation approaches in combination with substantial improvements in LC-MS platforms have enabled the plasma proteome to be profiled with considerably greater dynamic range of coverage, allowing many proteins at low ng/ml levels to be confidently identified. Despite these significant advances and efforts, major challenges associated with the dynamic range of measurements and extent of proteome coverage, confidence of peptide/protein identifications, quantitation accuracy, analysis throughput, and the robustness of present instrumentation must be addressed before a proteomics profiling platform suitable for efficient clinical applications can be routinely implemented.

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Section: Strategiesmentioning

confidence: 99%

Section: Strategiesmentioning

confidence: 99%

mentioning

confidence: 99%

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Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications

Qian

Jacobs

Liu

et al. 2006

Molecular & Cellular Proteomics

327

284

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show abstract

“…In case of respiratory tract infections local disease markers such as proteins present in bronchoalveolar lavage fluid (BALF) are likely to be most meaningful [13,35,53]. In order to identify potential markers, proteome analysis of BALF performed with two dimensional gel electrophoresis (2-D PAGE) followed by mass spectrometry has become the method of choice [37,52]. Following this approach, initial studies of HennigPauka et al [20,21] identified the antibacterial peptide PR-39 as a first marker for chronic respiratory tract infection in pigs.…”

Section: Introductionmentioning

confidence: 99%

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

D¹,

Buettner²,

Naim³

et al. 2009

Vet. Res.

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-Biomarkers facilitating both pathogen-independent diagnosis of respiratory health and breeding selection of pigs with increased resistance to respiratory tract infections would be of considerable interest to the pig industry. Following this concept we performed a comparative glycoproteome analysis of bronchoalveolar lavage fluid (BALF) from healthy pigs and pigs 4 days (acute) and 20 days (chronic) after an experimental infection with Actinobacillus pleuropneumoniae. In order to identify possible differences in BALF glycoprotein patterns we investigated pigs of three different breeding lines (German Landrace, Piétrain, Hampshire). In total, 12 glycosylated proteins (alpha-1-acid glycoprotein, fetuin A, properdin, haptoglobin precursor, haptoglobin, hemoglobin, hyaluronidase, inter-alpha-trypsin inhibitor family heavy chain-related protein, alpha-1-antichymotrypsin 3, pulmonary surfactant-associated protein D (SP-D), transferrin, and alpha-1B-glycoprotein) were identified as being differentially expressed depending on the health status of the animal. Fetuin A levels were consistently low in chronically infected pigs thereby being a potential marker for chronic infection. Hyaluronidase levels were consistently high in all pigs after experimental infection independent on isolation of the pathogen thereby being a potential marker for previous pathogen contact and latent infection. High levels of fetuin A as well as low levels of haptoglobin and pulmonary SP-D correlated with the absence of lung lesions in pigs of the Hampshire breeding line, implying a potential application as selection markers for breeding programmes.porcine respiratory disease / glycoprotein analysis / bronchoalveolar lavage fluid / biomarker

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“…Several articles have been published on the proteomic composition of human BALF, especially for adults (9)(10)(11)(12). Among detected proteins that are receiving further attention, especially for their function and involvement in acute and chronic inflammatory conditions, are the α-defensins and S100A member proteins (calgranulins) (13,14).…”

mentioning

confidence: 99%

Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates

et al. 2013

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Background: Candida mannan (Mn) detection in bronchoalveolar lavage fluid (BALF) was shown to be useful for earlier identification and preemptive therapy targeting in preterm infants at high risk of invasive Candida infection. We investigated whether early detection of Candida Mn in BALF is associated with the presence of some neutrophilic products, as markers of prenatal infection/inflammation. Methods: BALF specimens were collected during the first 48 h of life from mechanically ventilated preterm newborns. Samples were analyzed by high-performance liquid chromatography-electrospray ionization-mass spectrometry. The relative amounts of α-defensins 1-4 and S100A proteins were measured by extracted ion current peak area. Absolute and differential white cell counts in BALF were obtained. Mn antigen concentrations were determined by the Platelia Candida antigen kit. results: Twenty-five studied neonates were divided into two groups: Mn-positive group and Mn-negative group. Levels of α-defensins 1-4 and S100A12 were significantly higher in the Mn-positive group than in the Mn-negative group. Moreover, positive significant correlations between the absolute number of neutrophils and the levels of α-defensins 1-4 and S100A8 were observed. conclusion: The detection of Mn antigen in BALF of preterm infants is consistent with evidence of an innate immune response in their lungs as demonstrated by higher levels of α-defensins and S100A proteins.

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Proteomics of bronchoalveolar lavage fluid

Cited by 128 publications

References 72 publications

Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications

Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications

Glycoprotein analysis of porcine bronchoalveolar lavage fluid reveals potential biomarkers corresponding to resistance toActinobacillus pleuropneumoniaeinfection

Association of high levels of α-defensins and S100A proteins with Candida mannan detection in bronchoalveolar lavage fluid of preterm neonates

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