Proteomics in neurosciences

Drabik, Anna; Bierczyńska-Krzysik, Anna; Bodzoń‐Kułakowska, Anna; Suder, Piotr; Kotlińska, Jolanta; Silberring, Jerzy

doi:10.1002/mas.20131

Cited by 45 publications

(32 citation statements)

References 140 publications

(155 reference statements)

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“…Identification of disease-associated proteins in tissues that can serve as disease-specific biomarkers has been the goal of traditional proteomic studies 1 . Interstitial cystitis (IC) is a painful bladder syndrome of unclear etiology, associated with urinary frequency and urgency.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Investigation on Chronic Bladder Irritation in the Rat

Tyagi

Chen

Hayashi

et al. 2008

Urology

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Purpose-Interstitial cystitis (IC) is a painful bladder syndrome associated with urinary frequency and urgency. Elusive etiology of IC makes its diagnosis only possible by exclusion in many cases. In the present study, we used proteomics for identifying disease-associated proteins in rat model of chronic bladder irritation.Materials & Methods-Chronic irritation of rat bladder was caused by a brief (90 secs) intravesical instillation of 0.2ml of 0.4N HCl. Whole bladders were collected at different time points after treatment, snap frozen and nuclear and cytosolic protein extracts were obtained. Samples were resolved in standard 2D-gels stained with an improved Coomasie Stain or by Differential Gel Electrophoresis (DIGE). Differentially expressed spots were excised and identified by MALDI-TOF MS/MS. Histological and Western blot analysis were also performed.Results-Bladder morphology and histologic appearance of bladder sections following HCl treatment reflected hemorrhage, edema, epithelial denudation, detrusor mastocytosis and eosinophilia. Proteomic analysis of irritated rat bladder revealed marked overexpression of four nuclear proteins and marked underexpression of one nuclear protein compared to normal rat bladders. Among these proteins, inflammation-associated Calgranulin A (over) and smooth muscle protein-22/transgelin (under) showed opposed expression patterns following bladder irritation.Conclusions-Presence of mast cells and eosinophils and overexpression of calgranulin A confirm the inflammatory component of HCl-irritated bladder. Altered expression of nuclear proteins is of particular interest because of their possible role as a prognostic marker in inflammatory bladder disorders. However, more studies are needed before clinical application of these findings can be established.

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Section: Introductionmentioning

confidence: 99%

Proteomic Investigation on Chronic Bladder Irritation in the Rat

Tyagi

Chen

Hayashi

et al. 2008

Urology

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“…It is necessary to cross the barriers of limited resolution, mass range, detection level, and other reasons for protein underrepresentation in analyzed proteomes. Once achieved, the door that allows complete identification of specific protein markers will open and the comprehension of complex networks of protein/peptide interactions involved in cancer will begin to be elucidated [6] . While the application of computational and statistical methods to proteomic profiling is relatively new, it is rapidly gaining interest.…”

Section: Perspectivesmentioning

confidence: 99%

“…In the ''post-genomic era,'' proteomics might be the key to understand systems biology. During the past few years, proteomics has been utilized in many fields of science, medicine, pharmacy, industry and agriculture [6] . In most of the applications proteomics is used to determine expression profiles of proteins in cells and tissues in normal or disease states [7] that are responsible for abnormal cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

Moving forward in colorectal cancer research, what proteomics has to tell

Bitarte

Bandrés²,

Zarate³

et al. 2007

WJG

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Colorectal cancer is the third most common cancer and is highly fatal. During the last several years, research has been primarily based on the study of expression profiles using microarray technology. But now, investigators are putting into practice proteomic analyses of cancer tissues and cells to identify new diagnostic or therapeutic biomarkers for this cancer. Because the proteome reflects the state of a cell, tissue or organism more accurately, much is expected from proteomics to yield better tumor markers for disease diagnosis and therapy monitoring. This review summarizes the most relevant applications of proteomics the biomarker discovery for colorectal cancer.

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“…Although ALS is a rare disorder, it is an excellent model 560 T. Ekegren, J. Hanrieder and J. Bergquist human proteins are most probably increasing. 2 As genes encode proteins of basic biological functions, PTMs, such as phosphorylation, glycosylation and acetylation regulate the real-time dynamics of protein structure and function. Accordingly, more than 200 different PTMs have been reported.…”

Section: Introductionmentioning

confidence: 99%

Clinical perspectives of high‐resolution mass spectrometry–based proteomics in neuroscience—Exemplified in amyotrophic lateral sclerosis biomarker discovery research

2008

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Biomarker discovery is a central application in today's proteomic research. There is an urgent need for valid biomarkers to improve diagnostic tools and treatment in many disorders, such as the rapidly progressing neurodegenerative disorder amyotrophic lateral sclerosis (ALS) that has a fatal outcome in about 3 years and yet no curative treatment. Screening for clinically relevant biomarkers puts high demands on high-throughput, rapid and precise proteomic techniques. There is a large variety in the methods of choice involving mainly gel-based approaches as well as chromatographic techniques for multi-dimensional protein and peptide separations followed by mass spectrometry (MS) analysis. This special feature article will discuss some important aspects of MS-based clinical proteomics and biomarker discovery in the field of neurodegenerative diseases and ALS research respectively, with the aim to provide a prospective view on current and future research aspects in the field. Furthermore, examples for application of high-resolution MS-based proteomic strategies for ALS biomarker discovery will be demonstrated with two studies previously reported by our group. These studies include among others, utilization of capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR-MS) for advanced protein pattern classification in cerebrospinal fluid (CSF) samples of ALS patients as well as highly sensitive protein identification in minimal amounts of postmortem spinal cord tissue and laser micro-dissected motor neurons using FT-ICR-MS in conjunction with nanoflow LC coupled to matrix-assisted laser desorption ionization time-of-flight tandem mass spectrometry (LC-MALDI-TOF-TOF-MS).

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Proteomics in neurosciences

Cited by 45 publications

References 140 publications

Proteomic Investigation on Chronic Bladder Irritation in the Rat

Proteomic Investigation on Chronic Bladder Irritation in the Rat

Moving forward in colorectal cancer research, what proteomics has to tell

Clinical perspectives of high‐resolution mass spectrometry–based proteomics in neuroscience—Exemplified in amyotrophic lateral sclerosis biomarker discovery research

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