Proteomics for the diagnosis of thyroid lesions: preliminary report

Pagni, Fabio; Mainini, Veronica; Garancini, Mattia; Bono, F; Vanzati, Alice; Giardini, Valentina; Scardilli, Marcella; Goffredo, Paolo; Smith, Andrew; Galli, Manuel; Sio, Gabriele De; Magni, Fulvio

doi:10.1111/cyt.12166

Cited by 33 publications

(28 citation statements)

References 13 publications

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“…Mainini et al [69] performed a preliminary test on various types of lesions including cvPTC, fvPTC, hyperplastic nodules, and uncertain malignant potential tumors, attempting a classification of these conditions based on hierarchical cluster analysis and principal component analysis of protein expression spectra. Analog results were obtained by Pagni et al [70] in their feasibility study, applying MALDI imaging on a set of various lesions as hyperplastic nodules, Hurtle cell-type FTA, medullary thyroid carcinoma, and cvPTC, showing similar spectra of protein expression between entities with similar biological behavior.…”

Section: Proteomics On Thyroid Cytological Samplessupporting

confidence: 77%

“…The validation via ELISA and WB confirmed the role of these proteins in discriminating PTC from controls and ANXA1 showed a sensitivity of 87% and a specificity of 94% in distinguishing cancer from normal cases. Recent studies showed how MALDI-MSI can be used to investigate ex vivo FNA specimens [69,70]. Mainini et al [69] performed a preliminary test on various types of lesions including cvPTC, fvPTC, hyperplastic nodules, and uncertain malignant potential tumors, attempting a classification of these conditions based on hierarchical cluster analysis and principal component analysis of protein expression spectra.…”

Section: Proteomics On Thyroid Cytological Samplesmentioning

confidence: 99%

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Proteome analysis in thyroid pathology

Pagni

L’Imperio

Bono

et al. 2015

Expert Review of Proteomics

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The incidence of thyroid cancer has continuously increased due to its detection in the preclinical stage. Clinical research in thyroid pathology is focusing on the development of new diagnostic tools to improve the stratification of nodules that have biological, practical and economic consequences on the management of patients. Several clinical questions related to thyroid carcinoma remain open and the use of proteomic research in the hunt for new targets with potential diagnostic applications has an important role in the solutions. Many different proteomic approaches are used to investigate thyroid lesions, including mass spectrometry profiling and imaging technologies. These approaches have been applied to different human tissues (cytological specimens, frozen sections, formalin-fixed paraffin embedded tissue or Tissue Micro Arrays). Moreover, other specimens are used for biomarker discovery, such as cell lines and the secretome. Alternative approaches, such as metabolomics and lipidomics, are also used and integrated within proteomics.

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Section: Proteomics On Thyroid Cytological Samplessupporting

confidence: 77%

Section: Proteomics On Thyroid Cytological Samplesmentioning

confidence: 99%

Proteome analysis in thyroid pathology

Pagni

L’Imperio

Bono

et al. 2015

Expert Review of Proteomics

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“…Techniques such as matrix-assisted laser desorption/ionization (MALDI)-TOF-MS and MALDI-imaging mass spectrometry (MALDI-IMS) have been applied in several studies [90,91,92,93,94,95] to cytological thyroid specimens. Most of these studies used ex vivo FNA [90,91,93,94,95] and one study [94] used pre-surgical FNAC obtaining an overall sensitivity of 87% and specificity of 94% in discriminating benign from suspicious samples, with a good reproducibility among studies. Proteomics could serve to improve the preoperative diagnosis of indeterminate lesions, but some aspects such as the limitation in the availability of these technologies and the lack of uniformity among techniques, need to be addressed before its introduction in clinical practice.…”

Section: Proteomics: An Interesting Alternative Approach To Stratimentioning

confidence: 99%

Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis

Cantara

Marzocchi

Pilli

et al. 2017

IJMS

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Fine needle aspiration cytology (FNAC) represents the gold standard for determining the nature of thyroid nodules. It is a reliable method with good sensitivity and specificity. However, indeterminate lesions remain a diagnostic challenge and researchers have contributed molecular markers to search for in cytological material to refine FNAC diagnosis and avoid unnecessary surgeries. Nowadays, several “home-made” methods as well as commercial tests are available to investigate the molecular signature of an aspirate. Moreover, other markers (i.e., microRNA, and circulating tumor cells) have been proposed to discriminate benign from malignant thyroid lesions. Here, we review the literature and provide data from our laboratory on mutational analysis of FNAC material and circulating microRNA expression obtained in the last 6 years.

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“…Among the localized proteins, ribosomal protein P2 was found specific for cancerous tissues . Other preliminary studies by MSI were performed for thyroid fine needle aspiration smears .…”

Section: Applications Of Msi In Cancer Diagnosis Staging and Prognosismentioning

confidence: 99%

MALDI mass spectrometry imaging: A cutting‐edge tool for fundamental and clinical histopathology

Longuespée

Casadonte²,

Kriegsmann

et al. 2016

Proteomics Clinical Apps

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Histopathological diagnoses have been done in the last century based on hematoxylin and eosin staining. These methods were complemented by histochemistry, electron microscopy, immunohistochemistry (IHC), and molecular techniques. Mass spectrometry (MS) methods allow the thorough examination of various biocompounds in extracts and tissue sections. Today, mass spectrometry imaging (MSI), and especially matrix-assisted laser desorption ionization (MALDI) imaging links classical histology and molecular analyses. Direct mapping is a major advantage of the combination of molecular profiling and imaging. MSI can be considered as a cutting edge approach for molecular detection of proteins, peptides, carbohydrates, lipids, and small molecules in tissues. This review covers the detection of various biomolecules in histopathological sections by MSI. Proteomic methods will be introduced into clinical histopathology within the next few years.

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Proteomics for the diagnosis of thyroid lesions: preliminary report

Cited by 33 publications

References 13 publications

Proteome analysis in thyroid pathology

Proteome analysis in thyroid pathology

Molecular Signature of Indeterminate Thyroid Lesions: Current Methods to Improve Fine Needle Aspiration Cytology (FNAC) Diagnosis

MALDI mass spectrometry imaging: A cutting‐edge tool for fundamental and clinical histopathology

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