Proteomics-based identification of plasma biomarkers in oral squamous cell carcinoma

Tung, Chun‐Liang; Lin, Szu‐Ting; Chou, Hsiu‐Chuan; Chen, Yiwen; Lin, Hwan-Chung; Tung, Chung-Liang; Huang, Kao-Jean; Chen, Yi–Ju; Lee, Ying‐Ray; Chan, Hong‐Lin

doi:10.1016/j.jpba.2012.11.017

Cited by 37 publications

(18 citation statements)

References 25 publications

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“…We conducted gene ontology (GO) analysis for our previous transcriptome data of OSCC tumors to better understand the biological characteristics of the differentially expressed genes in OSCC. As noted by previous studies [12], [19], [38], cytokine and other immune-related signaling pathways were found to be highly enriched in the top ten GO terms (Figure 3A). Although GPCR signaling is important for the inflammatory response and invasiveness of cancers, it remains to be defined whether Gα 12 is required for the inflammation-associated tumorigenesis in OSCC.…”

Section: Resultssupporting

confidence: 82%

Gα12 Drives Invasion of Oral Squamous Cell Carcinoma through Up-Regulation of Proinflammatory Cytokines

et al. 2013

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Oral squamous cell carcinoma (OSCC) ranks among the top ten most prevalent cancers worldwide. Like most head and neck squamous cell carcinomas (HNSCCs), OSCC is highly inflammatory and aggressive. However, the signaling pathways triggering the activation of its inflammatory processes remain elusive. G protein-coupled receptor signaling regulates the inflammatory response and invasiveness of cancers, but it remains unclear whether Gα12 is a critical player in the inflammatory cytokine pathway during the tumorigenesis of OSCC. This study was undertaken to determine the role of Gα12 signaling in the regulation of proinflammatory cytokines in their mediation of OSCC invasion. We found that both the transcription and protein levels of Gα12 are up-regulated in OSCC tumors. The elevated Gα12 expressions in OSCC patients also correlated with extra-capsular spread, an indicator of tumor invasiveness in HNSCCs. This clinical finding was supported by the studies of overexpression and RNAi knockdown of Gα12 in OSCC cells, which demonstrated that Gα12 promoted tumor cell migration and invasion. To understand how Gα12 modulates OSCC invasiveness, we analyzed key biological processes in microarray data upon depletion of Gα12 and found that cytokine- and other immune-related pathways were severely impaired. Importantly, the mRNA levels of IL-6 and IL-8 proinflammatory cytokines in clinical samples were found to be significantly correlated with the increased Gα12 levels, suggesting a potential role of Gα12 in modulating the IL-6 and IL-8 expressions. Supporting this hypothesis, overexpression or RNAi knockdown of Gα12 in OSCC cell lines both showed that Gα12 positively regulated the mRNA and protein levels of IL-6 and IL-8. Finally, we demonstrated that the Gα12 promotion of tumor cell invasiveness was suppressed by the neutralization of IL-6 and IL-8 in OSCC cells. Together, these findings suggest that Gα12 drives OSCC invasion through the up-regulation of IL-6 and IL-8 cytokines.

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Section: Resultssupporting

confidence: 82%

Gα12 Drives Invasion of Oral Squamous Cell Carcinoma through Up-Regulation of Proinflammatory Cytokines

et al. 2013

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“…Another protein, the leucine-rich alpha-2-glycoprotein, was also found to be increased in samples of SUI patients. This protein is secreted and normally present in plasma; however, it was also described to be involved in nonspecific inflammatory and cancer processes [25][26][27][28]. It has recently been described in the context of ulcerative colitis activity, pediatric, invasive bladder cancer, biliary tract cancer, lung cancer, pancreatic cancer, heart failure, neutrophilic granulocyte differentiation, and autoimmune diseases [29,30].…”

Section: Proteins Identifiedmentioning

confidence: 99%

Stress Urinary Incontinence: A Proteomics Overview

Mitulović¹,

Mohr²,

Koch³

2020

Lower Urinary Tract Dysfunction - From Evidence to Clinical Practice

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Proteomics research offers one strategy to elucidate the etiology of stress urinary incontinence (SUI) by identification of a significant and sufficient number of proteins, which provides the ability to avoid a preselection of candidate proteins for a possible early detection of the SUI. SUI represents both a psychological as well as an economic burden, and prevalence rates are expected to increase in the future, due to increasing of life expectancy. The classical epidemiology of SUI is well understood, with many environmental and lifestyle risk factors identified, including age, obesity, parity, vaginal delivery, and family history. Despite this, much of the etiology of SUI remains unclear, and it is difficult to predict which women are at risk. This chapter shows some results based on proteomic analysis of the urine proteome, which might give the answer to the question on pathways activated in SUI. Besides proteins originating from the blood, urine contains proteins secreted from the inner wall of the bladder and the urethra, and these proteins might explain the processes involved in genesis of SUI.

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“…Subjective histopathological analysis would likely be improved by objectively relating pathological states to chemical information obtained by desorption electrospray ionization mass spectrometry (DESI-MS). 1,3,12,13 As established in several other organ systems, DESI-MS imaging of lipid profiles in tissue can be correlated with histopathological characterization. 14…”

Section: Desorption Electrospray Ionization Mass Spectrometry As a mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Feasibility of desorption electrospray ionization mass spectrometry for diagnosis of oral tongue squamous cell carcinoma

D'Hue

Moore

Summerlin

et al. 2017

Rapid Comm Mass Spectrometry

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Rationale: Desorption electrospray ionization mass spectrometry (DESI-MS) has demonstrated utility in differentiating tumor from adjacent normal tissue in both urologic and neurosurgical specimens. We sought to evaluate if this technique had similar accuracy in differentiating oral tongue squamous cell carcinoma (SCC) from adjacent normal epithelium due to current issues with late diagnosis of SCC in advanced stages.Methods: Fresh frozen samples of SCC and adjacent normal tissue were obtained by surgical resection. Resections were analyzed using DESI-MS sometimes by a blinded technologist.Normative spectra were obtained for separate regions containing SCC or adjacent normal epithelium. Principal Component Analysis and Linear Discriminant Analysis (PCA-LDA) of spectra were used to predict SCC versus normal tongue epithelium. Predictions were compared with pathology to assess accuracy in differentiating oral SCC from adjacent normal tissue.Results: Initial PCA score and loading plots showed clear separation of SCC and normal epithelial tissue using DESI-MS. PCA-LDA resulted in accuracy rates of 95% for SCC versus normal and 93% for SCC, adjacent normal and normal. Additional samples were blindly analyzed with PCA-LDA pixel-by-pixel predicted classifications as SCC or normal tongue epithelial tissue and compared against histopathology. The m/z 700-900 prediction model showed a 91% accuracy rate.Conclusions: DESI-MS accurately differentiated oral SCC from adjacent normal epithelium.Classification of all typical tissue types and pixel predictions with additional classifications should increase confidence in the validation model.

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Proteomics-based identification of plasma biomarkers in oral squamous cell carcinoma

Cited by 37 publications

References 25 publications

Gα12 Drives Invasion of Oral Squamous Cell Carcinoma through Up-Regulation of Proinflammatory Cytokines

Gα12 Drives Invasion of Oral Squamous Cell Carcinoma through Up-Regulation of Proinflammatory Cytokines

Stress Urinary Incontinence: A Proteomics Overview

Feasibility of desorption electrospray ionization mass spectrometry for diagnosis of oral tongue squamous cell carcinoma

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