Proteomics-based identification of autoantibody against heat shock protein 70 as a diagnostic marker in esophageal squamous cell carcinoma

Fujita, Yoshihisa; Nakanishi, Toyofumi; Miyamoto, Yoshiharu; Hiramatsu, Masako; Mabuchi, Hideaki; Miyamoto, Akiko; Shimizu, Akira; Takubo, Takayuki; Tanigawa, Nobuhiko

doi:10.1016/j.canlet.2008.01.013

Cited by 80 publications

(63 citation statements)

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“…Therefore, the identification of novel biomarkers for assisting the diagnosis and predicting the prognosis of patients with ESCC is urgently required. In recent years, substantial attention has been been paid to the identification of biomarker targets, such as p53 (22) and heat shock protein 70 (23). A previous study showed that CAPS overexpression was significantly associated with histological grade in endometrial cancer (24).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of calcyphosine is associated with poor prognosis in esophageal squamous cell carcinoma

Zhu

Yang

et al. 2017

Oncol Lett

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Abstract. Calcyphosine (CAPS), a calcium-binding protein, has been identified as a potential diagnostic and prognostic biomarker in several human carcinomas. However, little is known about CAPS in esophageal squamous cell carcinoma (ESCC). The present study aimed to investigate the expression levels of CAPS in ESCC tissues and evaluate its clinicopathological significance. Reverse transcription-quantitative polymerase chain reaction and immunohistochemical staining were conducted to detect the expression of CAPS in ESCC tissues and adjacent non-cancerous tissues. ESCC samples exhibited higher levels of CAPS mRNA than paired non-cancerous samples (P=0.0015), and the mRNA level of CAPS was positively associated with histological grade (P=0.0013) and tumor invasion depth (P=0.0206). In addition, Kaplan-Meier survival analysis revealed that patients with high CAPS expression experienced significantly shorter 5-year overall survival times than those with low CAPS expression (P=0.0112). Multivariate analysis demonstrated that CAPS protein expression was an independent prognostic biomarker for patients with ESCC. In conclusion, the findings of the present study demonstrated that CAPS may represent a novel diagnostic indicator and an independent prognostic biomarker in ESCC.

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Section: Discussionmentioning

confidence: 99%

Overexpression of calcyphosine is associated with poor prognosis in esophageal squamous cell carcinoma

Zhu

Yang

et al. 2017

Oncol Lett

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“…An alternative approach to identify novel tumor biomarkers is the assessment of immune response elicited by tumor antigen since the humoral immune response to cancer in humans has been evidenced by the identification of autoantibodies to a variety of intracellular and surface antigens in cancer patients with different types of tumors [Chen et al, 2007;Disis et al, 1997;Hong et al, 2004;Soussi, 2000]. In ESCC, a number of reports have documented the presence of autoantibodies in serum against various proteins, including p53, cytokeratins, myomegalin, TRIM21, peroxiredoxin VI proteins, Hsp70, and CDC25B [Bergqvist et al, 2001;Fujita et al, 2006;Fujita et al, 2008;Liu et al, 2008;Shimada et al, 2007;Shimada et al, 2005;Veale et al, 1988]. The proteomic-based approach to identify panels of tumor antigens and related autoantibodies was introduced by Brichory et al in 2001, which identified antiannexin I and II antibodies in sera from patients with lung cancer [Brichory et al, 2001].…”

Section: Two-dimensional Electrophoresis-based Proteomic Findings Of mentioning

confidence: 99%

“…The frequency of autoantibody against PRX VI was 50% (15/30) in ESCC, only 6.6% (2/30) in health controls and 3.3% (1/30) in colon cancer. Two years later, the same research group discovered augmented concentration of Hsp70 autoantibody in the serum of ESCC patients, which was significantly higher in ESCC patients than gastric and colon cancer, healthy controls [Fujita et al, 2008]. On the other hand, Liu et al used ESCC tissue protein extracts and autologous sera to search for autoantibodies in ESCC patients and identified autoantibody CDC25B [Liu et al, 2008].…”

Section: Two-dimensional Electrophoresis-based Proteomic Findings Of mentioning

confidence: 99%

Proteomic Study of Esophageal Squamous Cell Carcinoma

Qi¹,

Chiu²

2012

Proteomics - Human Diseases and Protein Functions

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“…Notably, anti-HSP70 autoantibodies have been detected in 94% of patients with esophageal cancer but less commonly in other cancers (11% and 20% for gastric and colon) [25]. Anti-HSP70 antibodies have been detected in subgroups of patients with juvenile idiopathic arthritis [26], multiple sclerosis [27], Graves's disease [28], Behçet's disease [29], and sudden sensorineural hearing loss [30] but the significance of this in these diseases has yet to be determined.…”

Section: Introductionmentioning

confidence: 99%

Chronic smoke exposure induces rheumatoid factor and anti‐heat shock protein 70 autoantibodies in susceptible mice and humans with lung disease

et al. 2012

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The impact of cigarette smoke (CS), a risk factor for rheumatoid arthritis (RA), on autoantibody production was studied in humans and mice with and without chronic lung disease (LD). Rheumatoid factor (RF), anti-cyclic citrullinated peptides (CCPs), and anti-HSP70 autoantibodies were measured in several mouse strains and in cohorts of smokers and nonsmokers with and without autoimmune disease. Chronic smoking-induced RFs in AKR/J mice, which are most susceptible to LD. RFs were identified in human smokers, preferentially in those with LD. Anti-HSP70 autoantibodies were identified in CS-exposed AKR/J mice but not in ambient air exposed AKR/J controls. Whereas inflammation could induce anti-HSP70 IgM, smoke exposure promoted the switch to anti-HSP70 IgG autoantibodies. Elevated anti-CCP autoantibodies were not detected in CS-exposed mice or smokers. AKR/J splenocytes stimulated in vitro by immune complexes (ICs) of HSP70/anti-HSP70 antibodies produced RFs. The CD91 scavenger pathway was required as anti-CD91 blocked the HSP70-IC-induced RF response. Blocking Toll-like receptors did not influence the HSP70-IC-induced RFs. These studies identify both anti-HSP70 and RFs as serological markers of smoke-related LD in humans and mice. Identification of these autoantibodies could suggest a common environmental insult, namely CS, in a number of different disease settings.Keywords: Autoantibodies r Heat shock protein 70 r Lung disease r Rheumatoid factor IntroductionSmoking is a very strong environmental risk factor that is linked to rheumatoid arthritis (RA) [1,2] and other autoimmune disCorrespondence: Dr. Marianna M. Newkirk e-mail: Marianna.newkirk@mcgill.ca eases [3][4][5]. Furthermore, there is little information on the association between the most common smoke-induced lung disease (LD), chronic obstructive pulmonary disease (COPD), and serological markers associated with autoimmunity.In North American Native (NAN) populations the prevalence of RA can be three times that of Caucasians [6]. Whereas smoking rates have decreased in the past decade in Caucasians, with 18% of Canadians >15 years of age smoking, 60-70% C 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu1052 Marianna M. Newkirk et al. Eur. J. Immunol. 2012. 42: 1051-1061 of NANs (residing mostly on reservations) smoke (Health Canada). Although smoking cessation can lower RA risk and disease burden, the risk continues 10 years after cessation [1] and similarly, prolonged smoking cessation is critical for the reduction of lung cancer [7]. Lung inflammation, oxidative stress, and protease burden remain elevated for several months after smoking cessation [8]. Early diagnosis of those at risk of smoking-related diseases ideally by the use of informative biomarkers is critical with sustained cessation an important goal. Rheumatoid factor (RF), used in the diagnosis of RA [9], is an autoantibody of any isotype that binds IgG Fc. Epidemiological studies have shown that RF-positive RA patients are most associated with an increased risk conferred f...

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Proteomics-based identification of autoantibody against heat shock protein 70 as a diagnostic marker in esophageal squamous cell carcinoma

Cited by 80 publications

References 50 publications

Overexpression of calcyphosine is associated with poor prognosis in esophageal squamous cell carcinoma

Overexpression of calcyphosine is associated with poor prognosis in esophageal squamous cell carcinoma

Proteomic Study of Esophageal Squamous Cell Carcinoma

Chronic smoke exposure induces rheumatoid factor and anti‐heat shock protein 70 autoantibodies in susceptible mice and humans with lung disease

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