Proteomics Approach to Identify Unique Xylem Sap Proteins in Pierce’s Disease-Tolerant Vitis Species

Basha, Sheikh M.; Mazhar, Hifza; Vasanthaiah, Hemanth K. N.

doi:10.1007/s12010-009-8620-1

Cited by 49 publications

(30 citation statements)

References 28 publications

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“…In higher plant species, OEEs are involved in the water-splitting reaction, which is part of the photosynthetic oxygen-evolving process in photosystem II (PSII) [39]. OEE has been associated with stress adaptation [40]. The presence of OEE1 during seed morphogenesis can be explained by the fact that the photosynthetic system is established and functional in embryos of A. platanoides , while seeds are still attached to the mother plant (whole seeds are green because of the chlorophyll).…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)

Leśniewska

Pawłowski

2014

IJMS

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The proteome of zygotic embryos of Acer platanoides L. was analyzed via high-resolution 2D-SDS-PAGE and MS/MS in order to: (1) identify significant physiological processes associated with embryo development; and (2) identify changes in the proteome of the embryo associated with the acquisition of seed dormancy. Seventeen spots were identified as associated with morphogenesis at 10 to 13 weeks after flowering (WAF). Thirty-three spots were associated with maturation of the embryo at 14 to 22 WAF. The greatest changes in protein abundance occurred at 22 WAF, when seeds become fully mature. Overall, the stage of morphogenesis was characterized by changes in the abundance of proteins (tubulins and actin) associated with the growth and development of the embryo. Enzymes related to energy supply were especially elevated, most likely due to the energy demand associated with rapid growth and cell division. The stage of maturation is crucial to the establishment of seed dormancy and is associated with a higher abundance of proteins involved in genetic information processing, energy and carbon metabolism and cellular and antioxidant processes. Results indicated that a glycine-rich RNA-binding protein and proteasome proteins may be directly involved in dormancy acquisition control, and future studies are warranted to verify this association.

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Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)

Leśniewska

Pawłowski

2014

IJMS

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“…These metabolic changes lead to the accumulation of different proteins and secondary metabolites in the xylem sap. Some of the proteins and secondary metabolites that accumulate in the xylem sap during xylem colonization include PR-1, PR-2, PR-3, PR-4, PR-5, peroxidases, proteases, xyloglucan-endotransglycosylase (XET), and xyloglucan-specific endoglucanase inhibitor protein (XEGIP), phenols, phytoalexins, and lignin-like compounds (Cooper et al, 1996; Hilaire et al, 2001; Rep et al, 2002,2003; Williams et al, 2002; Houterman et al, 2007; Basha et al, 2010; Gayoso et al, 2010). These compounds are known to contribute directly or indirectly to plant defense.…”

Section: Plant Defense Against Vascular Wilt Pathogensmentioning

confidence: 99%

The xylem as battleground for plant hosts and vascular wilt pathogens

Yadeta¹,

Thomma²

2013

Front. Plant Sci.

416

400

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Vascular wilts are among the most destructive plant diseases that occur in annual crops as well as in woody perennials. These diseases are generally caused by soil-borne bacteria, fungi, and oomycetes that infect through the roots and enter the water-conducting xylem vessels where they proliferate and obstruct the transportation of water and minerals. As a consequence, leaves wilt and die, which may lead to impairment of the whole plant and eventually to death of the plant. Cultural, chemical, and biological measures to control this group of plant pathogens are generally ineffective, and the most effective control strategy is the use of genetic resistance. Owing to the fact that vascular wilt pathogens live deep in the interior of their host plants, studies into the biology of vascular pathogens are complicated. However, to design novel strategies to combat vascular wilt diseases, understanding the (molecular) biology of vascular pathogens and the molecular mechanisms underlying plant defense against these pathogens is crucial. In this review, we discuss the current knowledge on interactions of vascular wilt pathogens with their host plants, with emphasis on host defense responses against this group of pathogens.

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“…Mass spectrometry based proteomics has successfully been used to study varietal differentiation [13,14], disease resistance [15,16], bud development [17], berry development [18][19][20], salt stress [14,21], water stress [14,22,23], herbicide treatment [24] and different photoperiods [25] in grapevine. However, there have as yet been very few proteomic studies with respect to temperature stress in grapevine.…”

Section: Significance Of the Studymentioning

confidence: 99%

Quantitative proteomic analysis of cabernet sauvignon grape cells exposed to thermal stresses reveals alterations in sugar and phenylpropanoid metabolism

et al. 2015

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Grapes (Vitis vinifera) are a valuable fruit crop and wine production is a major industry. Global warming and expanded range of cultivation will expose grapes to more temperature stresses in future. Our study investigated protein level responses to abiotic stresses, with particular reference to proteomic changes induced by the impact of four different temperature stress regimes, including both hot and cold temperatures, on cultured grape cells. Cabernet Sauvignon cell suspension cultures grown at 26°C were subjected to 14 h of exposure to 34 and 42°C for heat stress, and 18 and 10°C for cold stress. Cells from the five temperatures were harvested in biological triplicates and label-free quantitative shotgun proteomic analysis was performed. A total of 2042 non-redundant proteins were identified from the five temperature points. Fifty-five proteins were only detected in extreme heat stress conditions (42°C) and 53 proteins were only detected at extreme cold stress conditions (10°C). Gene Ontology (GO) annotations of differentially expressed proteins provided insights into the metabolic pathways that are involved in temperature stress in grape cells. Sugar metabolism displayed switching between alternative and classical pathways during temperature stresses. Additionally, nine proteins involved in the phenylpropanoid pathway were greatly increased in abundance at extreme cold stress, and were thus found to be cold-responsive proteins. All MS data have been deposited in the ProteomeXchange with identifier PXD000977 (http://proteomecentral.proteomexchange.org/dataset/PXD000977).

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Proteomics Approach to Identify Unique Xylem Sap Proteins in Pierce’s Disease-Tolerant Vitis Species

Cited by 49 publications

References 28 publications

Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)

Proteomic Analysis of Embryogenesis and the Acquisition of Seed Dormancy in Norway Maple (Acer platanoides L.)

The xylem as battleground for plant hosts and vascular wilt pathogens

Quantitative proteomic analysis of cabernet sauvignon grape cells exposed to thermal stresses reveals alterations in sugar and phenylpropanoid metabolism

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