Proteomics and Phosphoproteomics of Heat Stress-Responsive Mechanisms in Spinach

Zhao, Qi; Chen, Wenxin; Bian, Jiayi; Xie, Hao; Liu, Ying; Xu, Chenxi; Ma, Jun; Guo, Siyi; Chen, Jiaying; Cai, Xiaofeng; Wang, Xiaoli; Wang, Quanhua; She, Yi‐Min; Chen, Sixue; Zhou, Zhiqiang; Dai, Shaojun

doi:10.3389/fpls.2018.00800

Cited by 84 publications

(62 citation statements)

References 131 publications

(195 reference statements)

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“…The plant growth and development were seriously also affected by high temperature [65][66][67][68][69][70][71][72]. In our study, nineteen of Hsf gene family members, which were expressed in different tea tissues, were selected to further confirm their expression patterns in heat stress response.…”

Section: Qrt-pcr Analyses Of Tea Hsf Genes Under Heat Stress Conditionmentioning

confidence: 96%

New Insights into Evolution of Plant Heat Shock Factors (Hsfs) and Expression Analysis of Tea Genes in Response to Abiotic Stresses

Guo²,

Pang

et al. 2020

Plants

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Heat shock transcription factor (Hsf) is one of key regulators in plant abotic stress response. Although the Hsf gene family has been identified from several plant species, original and evolution relationship have been fragmented. In addition, tea, an important crop, genome sequences have been completed and function of the Hsf family genes in response to abiotic stresses was not illuminated. In this study, a total of 4208 Hsf proteins were identified within 163 plant species from green algae (Gonium pectorale) to angiosperm (monocots and dicots), which were distributed unevenly into each of plant species tested. The result indicated that Hsf originated during the early evolutionary history of chlorophytae algae and genome-wide genetic varies had occurred during the course of evolution in plant species. Phylogenetic classification of Hsf genes from the representative nine plant species into ten subfamilies, each of which contained members from different plant species, imply that gene duplication had occurred during the course of evolution. In addition, based on RNA-seq data, the member of the Hsfs showed different expression levels in the different organs and at the different developmental stages in tea. Expression patterns also showed clear differences among Camellia species, indicating that regulation of Hsf genes expression varied between organs in a species-specific manner. Furthermore, expression of most Hsfs in response to drought, cold and salt stresses, imply a possible positive regulatory role under abiotic stresses. Expression profiles of nineteen Hsf genes in response to heat stress were also analyzed by quantitative real-time RT-PCR. Several stress-responsive Hsf genes were highly regulated by heat stress treatment. In conclusion, these results lay a solid foundation for us to elucidate the evolutionary origin of plant Hsfs and Hsf functions in tea response to abiotic stresses in the future.

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Section: Qrt-pcr Analyses Of Tea Hsf Genes Under Heat Stress Conditionmentioning

confidence: 96%

New Insights into Evolution of Plant Heat Shock Factors (Hsfs) and Expression Analysis of Tea Genes in Response to Abiotic Stresses

Guo²,

Pang

et al. 2020

Plants

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“…The plant growth and development are seriously affected by abiotic stress [44][45][46], including high temperature and low temperature [47][48][49][50][51][52][53][54][55][56][57]. In the present study, based on available genome-wide RNA-seq data, we analyzed the expression profile of pepper STP genes under cold and high temperature stress treatments.…”

Section: Expression Changes Of Stp Genes In Pepper Seedlings Under Comentioning

confidence: 99%

Sugar transporter proteins in Capsicum: identification, characterization, evolution and expression patterns

Wei

Liu

Zheng³

et al. 2020

Biotechnology & Biotechnological Equipment

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Sugar transporter proteins (STPs) play an important role in plant growth and development and stress resistance. Pepper (Capsicum annuum L.) is a significant economic crop and is widely cultivated worldwide. However, the evolution and the roles of STP genes in the growth and development and in coping with abiotic stresses in pepper are poorly known. Here, we characterized STP gene family in pepper through integration of chromosomal location, gene structure, conserved motif, phylogeny, expression patterns in different tissues and abiotic stresses. Using bioinformatics-based methods, we identified 17 putative STP genes in pepper. Chromosome mapping indicated that they are unevenly distributed on 9 chromosomes and there are tandem duplication events. Gene structure analysis revealed that intron numbers among these CaSTP genes ranged from 2 to 5, except that CaSTP12 and CaSTP13 genes contained no introns. The phylogenetic tree of STP genes from pepper and other plant species revealed that STP genes were grouped into 4 subfamilies, suggesting that these gene subfamilies may have diverged from a single common ancestor prior to the mono-dicot split. Gene expression analysis based on RNA-seq data showed that three genes were expressed constitutively in all the tissues analyzed, implying that these genes might have specific function in pepper. Stress data analysis showed that the expression profiles of some members of the CaSTP gene family were altered under cold, heat, salt and hormone stress conditions. The present results will provide valuable information into the evolutionary relationships and functional divergence of the STP family in the future. ARTICLE HISTORY

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“…Analysis of AsA/DHA and GSH/GSSG: AsA and DHA were analysed according to Hodges et al (1996) and Zhao et al (2018). The ratio of AsA/DHA was expressed by the ratio of AsA content to DHA content.…”

Section: Assays Of Antioxidant Enzymesmentioning

confidence: 99%

Neodymium improves the activity of ascorbate-glutathione cycle and chloroplast function of wheat seedlings under chromium stress

Lu¹,

Wu²,

Yang³

et al. 2020

Photosynt.

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We investigated the roles of neodymium trichloride (NdCl3) in regulating ascorbate-glutathione (AsA-GSH) cycle and chloroplast function of chromium (Cr)-stressed wheat seedlings. The findings showed that Cr stress markedly increased malondialdehyde (MDA) content, electrolytic leakage (EL), nonphotochemical quenching, and the activities of enzymes in AsA-GSH cycle, compared with control. However, Cr stress markedly reduced the ratios of reduced ascorbate to dehydroascorbate and reduced glutathione to oxidized glutathione, net photosynthetic rate, the contents of chlorophylls and carotenoids, maximum photochemical efficiency of PSII, photochemical quenching, and quantum efficiency of PSII photochemistry, as well as plant height, root length, and plant biomass. NdCl3 plus Cr stress markedly reduced MDA content and EL and improved other indicators, compared with Cr stress alone. Meanwhile, NdCl3 alone also markedly improved above indicators except MDA and EL, compared with control. Current results implied that NdCl3 improved AsA-GSH cycle and chloroplast function of wheat seedlings under Cr stress.

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Proteomics and Phosphoproteomics of Heat Stress-Responsive Mechanisms in Spinach

Cited by 84 publications

References 131 publications

New Insights into Evolution of Plant Heat Shock Factors (Hsfs) and Expression Analysis of Tea Genes in Response to Abiotic Stresses

New Insights into Evolution of Plant Heat Shock Factors (Hsfs) and Expression Analysis of Tea Genes in Response to Abiotic Stresses

Sugar transporter proteins in Capsicum: identification, characterization, evolution and expression patterns

Neodymium improves the activity of ascorbate-glutathione cycle and chloroplast function of wheat seedlings under chromium stress

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