Proteomics and bioinformatics analysis of Fasciola hepatica somatic proteome in different growth phases

Abstract

Fasciola hepatica ( F. hepatica ) is a well-known zoonotic parasite that is crucial for economic and public health worldwide. Quantitative proteomics studies have been performed on proteins expressed by F. hepatica to investigate the differential expression of proteomes in different growth phases. And the screening of several marker proteins for use as early diagnostic antigens is essential. In this study, high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was conducted to analyze the differences in the expression of F. hepatica somatic proteins in different growth phases. Furthermore, gene ontology (GO) functional annotation, KEGG metabolic pathway, and clustering analyses were also performed. LC-MS/MS identified 629, 2286, 2254, and 2192 proteins in metacercariae, juvenile flukes 28dpi, immature flukes 59dpi, and adult phases, respectively. GO analysis revealed that differentially expressed proteins (DEPs) were mainly involved in transport, localization, metabolism, enzyme regulation, protein folding and binding, and nucleoside and nucleotide binding. The DEPs were enriched in cells, intracellular components, organelles, cytoplasm, vesicles, and membranes. KEGG pathway annotation results showed that the DEPs were involved in metabolism, genetic information processing, environmental information processing, cellular processes, organismal systems, and other processes. These findings provide a theoretical basis for vaccine development and establishing early diagnostic methods in the future. Electronic supplementary material The online version of this article (10.1007/s00436-020-06833-x) contains supplementary material, which is available to authorized users.

Can Cathepsin L Epitope Be an Effective Vaccine Candidate Against Fasciola gigantica via Bioinformatics?

“…hepatica Cat L protein using the sera of F. hepatica infected or vaccinated cattle in two independent experiments were identified, and these results were confirmed by other studies (35)(36)(37). The study also demonstrated that vaccinated animals with Cat L showed fluke burden reduction with eliciting antibodies (8).…”

De Novo Transcriptome Profiling of Naegleria fowleri Trophozoites and Cysts via RNA Sequencing

“…[ 20 ]; Clonorchis sinensis [ 21 , 22 , 23 ]; Schistosoma spp. [ 24 , 25 , 26 ]; and Fasciola hepatica [ 27 , 28 , 29 ]. To obtain transcriptomic insights into the differential expression of the stage-specific gene in N. fowleri , this study used RNA-Seq to analyze the transcriptome of N. fowleri cysts transferred to encystment buffer for 3 days compared with an N. fowleri trophozoite control group.…”

Perfil electroforético 2D de las proteínas del intestino de Fasciola hepatica

“…La fasciolosis es de gran importancia económica en todo el mundo considerada como una amenaza a nivel global para la salud pública, el bienestar animal, productividad agrícola y la seguridad alimentaria [42], causando enormes pérdidas económicas en la agricultura [4,10]. Según su cuadro clínico, puede clasificarse en dos fases: aguda y crónica, actualmente el diagnóstico se realiza durante la fase crónica, sin embargo, la detección temprana permitiría brindar un tratamiento eficaz y oportuno [33].…”