Proteomics analysis to compare the venom composition between <i>Naja naja</i> and <i>Naja kaouthia</i> from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom

Chanda, Abhishek; Patra, Aparup; Kalita, Bhargab; Mukherjee, Ashis K.

doi:10.1080/14789450.2018.1538799

Cited by 53 publications

(78 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of Ras-like protein demonstrates the presence of extracellular vesicles in the venom of Naja naja. The comparison of our proteomic data with that of N. naja snake both from western and eastern India, reveals that such proteins were not identified in Indian N. naja , Further in Pakistani Naja naja snake we not could identify cholinesterase, butyrylcholinesterase, hyalurinidase and snaclec proteins which were previously reported in Indian N. naja venom [ 45 , 46 ].…”

Section: Resultsmentioning

confidence: 59%

“…In Table 1 , the protein families discovered and reported for the first time in terms of our investigations are shown with check mark (✓). Interestingly previous studies reported PLA 2 as the second most abundant protein family found in N. naja venom, and that SVMP was present in relatively low abundance [ 8 , 42 , 43 , 44 , 45 , 46 ]. In contrast, our data showed SVMP as the second most abundant protein family in N. naja.…”

Section: Resultsmentioning

confidence: 99%

“…In contrast to Pakistani N. naja venom proteome, the eastern Indian N. naja venom comprises of 61% 3FTXs and western Indian N. naja contains 68% 3FTXs. Interestingly eastern Indian N. naja lacks short neurotoxins, which are present in both western Indian and Pakistani N. naja snake venom [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…A recent study reported the comparative enzymatic activity of PLA 2 s in ten different Naja species, with highest activity in N. siamensis and lowest in N. nivea [ 73 ]. The venom proteome study of Indian N. naja venom carried out by A. K. Mukherjee research group reported that Indian N. naja contains 20–27% PLA 2 s [ 45 , 46 ]. This is much higher than the amount of PLA2s present in Pakistani N. naja .…”

Section: Discussionmentioning

confidence: 99%

“…These research groups performed pre fractionation of the venom sample either by reverse phase chromatography, 1-dimensional gel electrophoresis (1D gel) or 2-dimensional gel electrophoresis (2 D gel) or a combination of these methods. Further mass spectrometric analysis of peptide fragments obtained from in gel trypsin digestion, was carried out by MALDI TOF/TOF, ion trap or ESI MS. Chanda et al also reported the venom proteomics of N. naja , from Western and Eastern parts of India [ 45 , 46 ]. In their study of the venom sample from East India, they pre fractionated the crude venom by 1D gel prior to LTQ orbitrap analysis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles

Manuwar

Dreyer

Böhmert

et al. 2020

Toxins

View full text Add to dashboard Cite

Latest advancement of omics technologies allows in-depth characterization of venom compositions. In the present work we present a proteomic study of two snake venoms of the genus Naja i.e., Naja naja (black cobra) and Naja oxiana (brown cobra) of Pakistani origin. The present study has shown that these snake venoms consist of a highly diversified proteome. Furthermore, the data also revealed variation among closely related species. High throughput mass spectrometric analysis of the venom proteome allowed to identify for the N. naja venom 34 protein families and for the N. oxiana 24 protein families. The comparative evaluation of the two venoms showed that N. naja consists of a more complex venom proteome than N. oxiana venom. Analysis also showed N-terminal acetylation (N-ace) of a few proteins in both venoms. To the best of our knowledge, this is the first study revealing this posttranslational modification in snake venom. N-ace can shed light on the mechanism of regulation of venom proteins inside the venom gland. Furthermore, our data showed the presence of other body proteins, e.g., ankyrin repeats, leucine repeats, zinc finger, cobra serum albumin, transferrin, insulin, deoxyribonuclease-2-alpha, and other regulatory proteins in these venoms. Interestingly, our data identified Ras-GTpase type of proteins, which indicate the presence of extracellular vesicles in the venom. The data can support the production of distinct and specific anti-venoms and also allow a better understanding of the envenomation and mechanism of distribution of toxins. Data are available via ProteomeXchange with identifier PXD018726.

show abstract

Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles

Manuwar

Dreyer

Böhmert

et al. 2020

Toxins

View full text Add to dashboard Cite

show abstract

The application of laboratory‐based analytical tools and techniques for the quality assessment and improvement of commercial antivenoms used in the treatment of snakebite envenomation

Patra

Herrera

Gutiérrez

et al. 2021

Drug Testing and Analysis

Self Cite

View full text Add to dashboard Cite

Snakebite envenomation is a public health problem of high impact, particularly for the developing world. Antivenom, which contains whole or protease‐digested immunoglobulin G, purified from the plasma of hyper‐immunized animals (mainly horses), is the mainstay for the treatment of snakebite envenomation. The success of antivenom therapy depends upon its ability to abrogate or reduce the local and systemic toxicity of envenomation. In addition, antivenom administration must be safe for the patients. Therefore, antivenom manufacturers must ensure that these products are effective and safe in the treatment of envenomations. Antivenom efficacy and safety are determined by the physicochemical characteristics of formulations, purity of the immunoglobulin fragments and antibodies, presence of protein aggregates, endotoxin burden, preservative load, and batch to batch variation, as well as on the ability to neutralize the most important toxins of the venoms against which the antivenom is designed. In this context, recent studies have shown that laboratory‐based simple analytical techniques, for example, size exclusion chromatography, sodium dodecyl sulphate polyacrylamide gel electrophoresis, mass spectrometry, immunological profiling including immuno‐turbidimetry and enzyme‐linked immunosorbent assays, Western blotting, immune‐chromatographic technique coupled to mass spectrometry analysis, reverse‐phase high performance liquid chromatography, spectrofluorometric analysis, in vitro neutralization of venom enzymatic activities, and other methodologies, can be applied for the assessment of antivenom quality, safety, stability, and efficacy. This article reviews the usefulness of different analytical techniques for the quality assessment of commercial antivenoms. It is suggested that these tests should be applied for screening the quality of commercial antivenoms before their preclinical and clinical assessment.

show abstract

A strategy for the enrichment and characterization of disulfide bond‐contained proteins from Chinese cobra (Naja atra) venom

Wei

Liu

Zheng

et al. 2021

J of Separation Science

View full text Add to dashboard Cite

A new strategy combined gold-coated magnetic nanocomposites assisted enrichment with mass spectrometry was developed for the characterization of disulfide bond-contained proteins from Chinese cobra (Naja atra) venom. In this work, core-shell nanocomposites were synthesized by the seed-mediated growth method and used for the enrichment of snake venom proteins containing disulfide bonds. A total of 3545 tryptic digested peptides derived from 96 venom proteins in Naja atra venom were identified. The venom proteins comprised 14 toxin families including three-finger toxins, phospholipase A 2 , snake venom metalloproteinase, cobra venom factor, and so forth. Extra 16 venom proteins were detected exclusively in the nanocomposites set, among which 11 venom proteins were from the three-finger toxins family. In the present study, the proposed simple and efficient protocol replaced the tedious and laborious technologies commonly used for pre-separating crude snake venom, suggesting widely implementation in low-abundance or trace disulfide bond-contained proteins or peptides characterization.

show abstract

Proteomics analysis to compare the venom composition between Naja naja and Naja kaouthia from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom

Cited by 53 publications

References 47 publications

Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles

Proteomic Investigations of Two Pakistani Naja Snake Venoms Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles

The application of laboratory‐based analytical tools and techniques for the quality assessment and improvement of commercial antivenoms used in the treatment of snakebite envenomation

A strategy for the enrichment and characterization of disulfide bond‐contained proteins from Chinese cobra (Naja atra) venom

Contact Info

Product

Resources

About