Proteomics Analysis Reveals Post-Translational Mechanisms for Cold-Induced Metabolic Changes in Arabidopsis

Li, Tian; Xu, Shou-Ling; Oses-Prieto, Juan A.; Putil, Sunita; Xu, Peng; Wang, Rui-Ju; Li, Kathy H.; Maltby, David; An, Lizhe; Burlingame, Alma L.; Deng, Zhiping; Wang, Zhiyong

doi:10.1093/mp/ssq078

Cited by 47 publications

(30 citation statements)

References 73 publications

(131 reference statements)

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“…Maltose is hydrolyzed from starch in the chloroplast and could be exported into the cytosol by its specific transporter MEX1 and catabolized by DPE2. Interestingly, recent studies suggested that both MEX1 and DPE2 influence plant cold tolerance by modulating the allocation of maltose (Li et al 2011;Lloyd et al 2004;Purdy et al 2013). Our results revealed that starch degradation is important for LT stimulation during CA period in tea plant.…”

Section: Ca Activates Starch Degradation In Response To Coldsupporting

confidence: 47%

“…The contributions of these genes to cold tolerance have been verified in different species. Several reports show that LT induces starch degradation, and the involved main enzymes coding genes in this pathway including glucan water dikinase (GWD), beta-amylase (BAM) as well as its downstream genes, such as maltose transporter (maltose excess1, MEX1) and disproportionating enzyme 2 (DPE2), are differentially regulated and facilitate plant cold responses Guy 2004, 2005;Li et al 2011;Purdy et al 2013;Yano et al 2005). For instance, PtrBAM1 has been identified as a CBF regulon, which functions under cold conditions (Peng et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

et al. 2015

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Sugar plays an essential role in plant cold acclimation (CA), but the interaction between CA and sugar remains unclear in tea plants. In this study, during the whole winter season, we investigated the variations of sugar contents and the expression of a large number of sugar-related genes in tea leaves. Results indicated that cold tolerance of tea plant was improved with the development of CA during early winter season. At this stage, starch was dramatically degraded, whereas the content of total sugars and several specific sugars including sucrose, glucose and fructose were constantly elevated. Beyond the CA stage, the content of starch was maintained at a low level during winter hardiness (WH) period and then was elevated during de-acclimation (DC) period. Conversely, the content of sugar reached a peak at WH stage followed by a decrease during DC stage. Moreover, gene expression results showed that, during CA period, sugar metabolism-related genes exhibited different expression pattern, in which beta-amylase gene (CsBAM), invertase gene (CsINV5) and raffinose synthase gene (CsRS2) engaged in starch, sucrose and raffinose metabolism respectively were solidly up-regulated; the expressions of sugar transporters were stimulated in general except the down-regulations of CsSWEET2, 3, 16, CsERD6.7 and CsINT2; interestingly, the sugar-signaling related CsHXK3 and CsHXK2 had opposite expression patterns at the early stage of CA. These provided comprehensive insight into the effects of CA on carbohydrates indicating that sugar accumulation contributes to tea plant cold tolerance during winter season, and a simply model of sugar regulation in response to cold stimuli is proposed.

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Section: Ca Activates Starch Degradation In Response To Coldsupporting

confidence: 47%

Section: Introductionmentioning

confidence: 99%

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

et al. 2015

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“…2D-DIGE has been successfully applied to investigate symbiosis- and pathogenesis-related protein in Medicago truncatula [16, 17] and to study the impact of abiotic stresses such as drought in oak [18], frost in Arabidopsis [19], ozone, and heavy metals in poplar [20–22]. …”

Section: Gel-based Proteomicsmentioning

confidence: 99%

Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance

Abdallah

Dumas‐Gaudot

Renaut³

et al. 2012

International Journal of Plant Genomics

172

175

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Two-dimensional gel electrophoresis (2-DE) is widely applied and remains the method of choice in proteomics; however, pervasive 2-DE-related concerns undermine its prospects as a dominant separation technique in proteome research. Consequently, the state-of-the-art shotgun techniques are slowly taking over and utilising the rapid expansion and advancement of mass spectrometry (MS) to provide a new toolbox of gel-free quantitative techniques. When coupled to MS, the shotgun proteomic pipeline can fuel new routes in sensitive and high-throughput profiling of proteins, leading to a high accuracy in quantification. Although label-based approaches, either chemical or metabolic, gained popularity in quantitative proteomics because of the multiplexing capacity, these approaches are not without drawbacks. The burgeoning label-free methods are tag independent and suitable for all kinds of samples. The challenges in quantitative proteomics are more prominent in plants due to difficulties in protein extraction, some protein abundance in green tissue, and the absence of well-annotated and completed genome sequences. The goal of this perspective assay is to present the balance between the strengths and weaknesses of the available gel-based and -free methods and their application to plants. The latest trends in peptide fractionation amenable to MS analysis are as well discussed.

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“…Alteration of energy metabolism under cold stress conditions can be affected by enzymes involved in glycolysis (Yan et al, 2006) and the tricarboxylic acid (TCA) cycle (Li et al, 2011). SCaMC mediates ATP-Mg/Pi exchange and plays important roles in energy metabolism (Yang et al, 2014).…”

Section: Involvement In Energy Metabolismmentioning

confidence: 99%

Proteomic Analysis of Cold Stress Responses in Banana Leaves

Ren¹,

Zhang²,

Wang³

et al. 2015

J. Amer. Soc. Hort. Sci.

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Cold stress is one of the most important environmental factors affecting crop growth and agricultural production. Induced changes of gene expression and metabolism are critical for plants responding and acclimating to cold stress. Banana (Musa sp.) is one of the most important food crops in the tropical and subtropical countries of the world. Banana, which originated from tropical regions, is sensitive to cold, which can result in serious losses in commercial banana production. To investigate the response of the banana to cold stress conditions, changes in protein expression were analyzed using a comparative proteomics approach. ‘Brazil’ banana (Musa acuminata AAA group) is a common banana cultivar in southern China. ‘Brazil’ banana plantlets were exposed to 5 °C for 24 hours and then total crude protein was extracted from treatment and control leaves by phenol extraction, separated with two-dimensional gel electrophoresis, and subsequently identified by mass spectrometry (MS). Out of the more than 400 protein spots reproducibly detected, only 41 protein spots exhibited a change in intensity by at least 2-fold, with 26 proteins increasing and 15 proteins decreasing expression. Of these, 28 differentially expressed proteins were identified by MS. The identified proteins, including well-known and novel cold-responsive proteins, are involved in several cellular processes, including antioxidation and antipathogen, photosynthesis, chaperones, protein synthesis, signal transduction, energy metabolism, and other cellular functions. Proteins related to antioxidation, pathogen resistance, molecular chaperones, and energy metabolism were up-regulated, and proteins related to ethylene synthesis, protein synthesis, and epigenetic modification were down-regulated in response to cold temperature treatment. The banana plantlets incubated at cold temperatures demonstrated major changes in increased reactive oxygen species (ROS) scavenging, defense against diseases, and energy supply. Increased antioxidation capability in banana was also discovered in plantain, which has greater cold tolerance than banana in response to cold stress conditions. Therefore, we hypothesized that an increased antioxidation ability could be a common characteristic of banana and plantain in response to cold stress conditions. These findings may provide a better understanding of the physiological processes of banana in response to cold stress conditions.

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Proteomics Analysis Reveals Post-Translational Mechanisms for Cold-Induced Metabolic Changes in Arabidopsis

Cited by 47 publications

References 73 publications

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance

Proteomic Analysis of Cold Stress Responses in Banana Leaves

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