Proteomics analysis of liver tissues from C57BL/6J mice receiving low-dose 137Cs radiation

Liang, Yi; Li, Linwei; Yin, Jie; Hu, Nan; Li, Guangyue; Ding, Dexin

doi:10.1007/s11356-015-5494-3

Cited by 11 publications

(8 citation statements)

References 28 publications

(24 reference statements)

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“…In our previous reports, GSTP1 and CAT proteins were up-regulated in all three experimental groups after 90 days of exposure [ 15 ]. Considerable evidence in recent years has suggested that CRT is involved in tumorigenesis.…”

Section: Materials Design and Methodsmentioning

confidence: 66%

“…GSTP1 is, with many functions, an acrolein-metabolizing enzyme [ 20 ]. In our previous reports, GSTP1 and CAT proteins were up-regulated in three experimental groups for 90 days [ 15 ]. In this study, CAT and GSTP1 in the irradiation groups also had a higher expression than those in the matched group.…”

Section: Discussionmentioning

confidence: 97%

“…Quantitative proteomics techniques have been well used to determine tumor markers [ 13 , 14 ]. Through proteomics analysis, our previous research identified differentially expressed proteins in liver tissues of mice that had received 137 Cs radiation for 90 d [ 15 ]. The purpose of this study was to determine biomarkers of radiation exposure using quantitative proteomics for use as early warning detection of radiation pollution.…”

Section: Introductionmentioning

confidence: 99%

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Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays

Liang

Yin

et al. 2017

PLoS ONE

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The biological effects of low-dose or low-dose-rate ionizing radiation on normal tissues has attracted attention. Based on previous research, we observed the morphology of liver tissues of C57BL/6J mice that received <50, 50–500, and 500–1000 μGy/h of 137Cs radiation for 180 d. We found that the pathological changes in liver tissues were more obvious as the irradiation dose rates increased. Additionally, differential protein expression in liver tissues was analyzed using a proteomics approach. Compared with the matched group in the 2D gel analysis of the irradiated groups, 69 proteins had ≥ 1.5-fold changes in expression. Twenty-three proteins were selected based on ≥2.5-fold change in expression, and 22 of them were meaningful for bioinformatics and protein fingerprinting analysis. These molecules were relevant to cytoskeleton processes, cell metabolism, biological defense, mitochondrial damage, detoxification and tumorigenesis. The results from real-time PCR and western blot (WB) analyses showed that calreticulin (CRT) was up-regulated in the irradiated groups, which indicates that CRT may be relevant to stress reactions when mouse livers are exposed to low-dose irradiation and that low-dose-rate ionizing radiation may pose a cancer risk. The CRT protein can be a potential candidate for low-dose or low-dose-rate ionizing radiation early-warning biomarkers. However, the underlying mechanism requires further investigation.

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Section: Materials Design and Methodsmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays

Liang

Yin

et al. 2017

PLoS ONE

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“…CATA acts by catalyzing the conversion of hydrogen peroxide into oxygen and water, and has been shown to protect irradiated tumor cells from apoptosis [45] . A previous study showed that CATA is increased in liver tissue of mice exposed to 137 Cs [46] .The peripherally increased levels of CATA in the tumors responding well to the treatment could thus be due to the oxidative stress imposed by irradiation. The association between CATA and D thus seems reasonable and supports the motivation to use perfusion-corrected diffusion measures to evaluate therapy response after radiotherapy.…”

Section: Discussionmentioning

confidence: 92%

Identification of Potential MR-Derived Biomarkers for Tumor Tissue Response to 177Lu-Octreotate Therapy in an Animal Model of Small Intestine Neuroendocrine Tumor

Montelius

Spetz

Jalnefjord

et al. 2018

Translational Oncology

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Magnetic resonance (MR) methods enable noninvasive, regional tumor therapy response assessment, but associations between MR parameters, underlying biology, and therapeutic effects must be investigated. The aim of this study was to investigate response assessment efficacy and biological associations of MR parameters in a neuroendocrine tumor (NET) model subjected to radionuclide treatment. Twenty-one mice with NETs received 177Lu-octreotate at day 0. MR experiments (day −1, 1, 3, 8, and 13) included T2-weighted, dynamic contrast-enhanced (DCE) and diffusion-weighted imaging (DWI) and relaxation measurements (T1/T2*). Tumor tissue was analyzed using proteomics. MR-derived parameters were evaluated for each examination day and for different radial distances from the tumor center. Response assessment efficacy and biological associations were evaluated using feature selection and protein expression correlations, respectively. Reduced tumor growth rate or shrinkage was observed until day 8, followed by reestablished growth in most tumors. The most important MR parameter for response prediction was DCE-MRI–derived pretreatment signal enhancement ratio (SER) at 40% to 60% radial distance, where it correlated significantly also with centrally sampled protein CCD89 (association: DNA damage and repair, proliferation, cell cycle arrest). The second most important was changed diffusion (D) between day −1 and day 3, at 60% to 80% radial distance, where it correlated significantly also with peripherally sampled protein CATA (association: oxidative stress, proliferation, cell cycle arrest, apoptotic cell death). Important information regarding tumor biology in response to radionuclide therapy is reflected in several MR parameters, SER and D in particular. The spatial and temporal information provided by MR methods increases the sensitivity for tumor therapy response.

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“…Through proteomics analysis, our previous research identified differentially expressed proteins in the liver tissues of mice that had received different doses of Cs-137 radiation for 90 and 180 days [ 22 , 23 ]. It is well known that α and γ radiations associated with UT also have the potential to harm workers.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings

Liang

et al. 2018

Bioscience Reports

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Uranium tailings (UT) are formed as a byproduct of uranium mining and are of potential risk to living organisms. In the present study, we sought to identify potential biomarkers associated with chronic exposure to low dose rate γ radiation originating from UT. We exposed C57BL/6J mice to 30, 100, or 250 μGy/h of gamma radiation originating from UT samples. Nine animals were included in each treatment group. We observed that the liver central vein was significantly enlarged in mice exposed to dose rates of 100 and 250 μGy/h, when compared with nonirradiated controls. Using proteomic techniques, we identified 18 proteins that were differentially expressed (by a factor of at least 2.5-fold) in exposed animals, when compared with controls. We chose glycine N-methyltransferase (GNMT), glutathione S-transferase A3 (GSTA3), and nucleophosmin (NPM) for further investigations. Our data showed that GNMT (at 100 and 250 μGy/h) and NPM (at 250 μGy/h) were up-regulated, and GSTA3 was down-regulated in all of the irradiated groups, indicating that their expression is modulated by chronic gamma radiation exposure. GNMT, GSTA3, and NPM may therefore prove useful as biomarkers of gamma radiation exposure associated with UT. The mechanisms underlying those changes need to be further studied.

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Proteomics analysis of liver tissues from C57BL/6J mice receiving low-dose 137Cs radiation

Cited by 11 publications

References 28 publications

Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays

Up-regulation of calreticulin in mouse liver tissues after long-term irradiation with low-dose-rate gamma rays

Identification of Potential MR-Derived Biomarkers for Tumor Tissue Response to 177Lu-Octreotate Therapy in an Animal Model of Small Intestine Neuroendocrine Tumor

Differential expression of NPM, GSTA3, and GNMT in mouse liver following long-term in vivo irradiation by means of uranium tailings

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