Proteomics analysis of hip articular cartilage identifies differentially expressed proteins associated with osteonecrosis of the femoral head

Song, Jidong; Wu, Junlong; Poulet, Blandine; Liang, Jing; Bai, Chuanyi; Dang, Xiaoqian; Wang, Kanming; Fan, Lihong; Liu, R.

doi:10.1016/j.joca.2021.04.005

Cited by 14 publications

(4 citation statements)

References 48 publications

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“…To validate our proteomic sequencing results, we employed Western blot to determine the expressions of the selected key proteins in ONFH and normal cancellous bone tissue samples [ 17 ]. Results showed the expressions of proteins such as C3, MMP9, MPO, LCN2, ELANE, and APOE significantly increased in the ONFH samples.…”

Section: Resultsmentioning

confidence: 99%

Exploring molecular mechanisms of intra-articular changes in osteonecrosis of femoral head using DIA proteomics and bioinformatics

Zhao,

Liu,

Zheng

et al. 2024

J Orthop Surg Res

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Purpose This study is aimed to delve into the crucial proteins associated with hormonal osteonecrosis of the femoral head (ONFH) and its intra-articular lesions through data-independent acquisition (DIA) proteomics and bioinformatics analysis. Methods We randomly selected samples from eligible ONFH patients and collected samples from the necrotic area of the femoral head and load-bearing cartilage. The control group comprised specimens from the same location in patients with femoral neck fractures. With DIA proteomics, we quantitatively and qualitatively tested both groups and analyzed the differentially expressed proteins (DEPs) between groups. Additionally, we enriched the analysis of DEP functions using gene ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways and verified the key proteins in ONFH through Western blot. Results Proteomics experiment uncovered 937 common DEPs (422 upregulated and 515 downregulated) between the two groups. These DEPs mainly participate in biological processes such as hidden attributes, catalytic activity, molecular function regulators, and structural molecule activity, and in pathways such as starch and sucrose metabolism, ECM–receptor interaction, PI3K-Akt signaling, complement and coagulation cascades, IL-17 signaling, phagosome, transcriptional misregulation in cancers, and focal adhesion. Through protein–protein interaction network target gene analysis and Western blot validation, we identified C3, MMP9, APOE, MPO, LCN2, ELANE, HPX, LTF, and THBS1 as key proteins in ONFH. Conclusions With DIA proteomics and bioinformatics analysis, this study reveals the molecular mechanisms of intra-articular lesions in ONFH. A correlation in the necrotic area and load-bearing cartilage of ONFH at ARCO stages IIIB-IV as well as potential key regulatory proteins was identified. These findings will help more deeply understand the pathogenesis of ONFH and may provide important clues for seeking more effective treatment strategies.

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Section: Resultsmentioning

confidence: 99%

Exploring molecular mechanisms of intra-articular changes in osteonecrosis of femoral head using DIA proteomics and bioinformatics

Zhao,

Liu,

Zheng

et al. 2024

J Orthop Surg Res

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“…5 B, p < 0.01). Therefore, decreasing the expression of important pro-inflammatory cytokines is critical to prevent the loss of articular cartilage to attenuate the osteoarthritis of hip joint in ONFH after treatments [ 11 , 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head

et al. 2023

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“…ONFH ( Chen et al, 2015a ). Previous studies have focused on the change of protein in bone tissue and cartilage ( Zeng et al, 2019 ; Song et al, 2021 ). Admittedly, there have been many proteomic studies on various pathological tissues of ONFH.…”

Section: Discussionmentioning

confidence: 99%

Serum Amyloid A Correlates With the Osteonecrosis of Femoral Head by Affecting Bone Metabolism

Peng

Wang

et al. 2021

Front. Pharmacol.

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Osteonecrosis of femoral head (ONFH) is a progressive hip joint disease without disease-modifying treatment. Lacking understanding of the pathophysiological process of ONFH has become the humper to develop therapeutic approach. Serum amyloid A (SAA) is an acute phase lipophilic protein during inflammation and we found that SAA is increased for the first time in the serum of ONFH patients through proteomic studies and quantitatively verified by ELISA. Treating rBMSCs with SAA inhibited the osteogenic differentiation via Wnt/β-catenin signaling pathway deactivation and enhanced the adipogenic differentiation via MAPK/PPARγ signaling pathway activation. Finally, bilateral critical-sized calvarial-defect rat model which received SAA treated rBMSCs demonstrated reduction of bone formation when compared to untreated rBMSCs implantation control. Hence, SAA is a vital protein in the physiological process of ONFH and can act as a potential therapeutic target to treat ONFH.

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Proteomics analysis of hip articular cartilage identifies differentially expressed proteins associated with osteonecrosis of the femoral head

Cited by 14 publications

References 48 publications

Exploring molecular mechanisms of intra-articular changes in osteonecrosis of femoral head using DIA proteomics and bioinformatics

Exploring molecular mechanisms of intra-articular changes in osteonecrosis of femoral head using DIA proteomics and bioinformatics

Effect of three clinical therapies on cytokines modulation in the hip articular cartilage and bone improvement in rat early osteonecrosis of the femoral head

Serum Amyloid A Correlates With the Osteonecrosis of Femoral Head by Affecting Bone Metabolism

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