Proteomics analysis of Cyclobalanopsis gilva provides new insights of low seed germination

Zaynab, Madiha; Pan, Dezhuo; Fatima, Mahpara; Sharif, Yasir; Chen, Shi-Pin; Chen, Wei

doi:10.1016/j.biochi.2020.10.008

Cited by 17 publications

(14 citation statements)

References 59 publications

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“…The comprehensive knowledge about reactions of RT-qPCR has been reported in our earlier published work ( Zaynab et al, 2021a ). In our study, the expression data were evaluated by using the 2 –ΔΔCT methods ( Zaynab et al, 2021c ), and the elongation factor 1-alpha was chosen as a housekeeping gene ( Zaynab et al, 2021b ). The primers used in this study are given in Supplementary Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

et al. 2022

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Germin and germin-like proteins (GLPs) perform a significant role in plants against biotic and abiotic stress. To understand the role of GLPs in potato, a comprehensive genome-wide analysis was performed in the potato genome. This study identified a total of 70 StGLPs genes in the potato genome, distributed among 11 chromosomes. Phylogenetic analysis exhibited that StGLPs were categorized into six groups with high bootstrap values. StGLPs gene structure and motifs analysis showed a relatively well-maintained intron–exon and motif formation within the cognate group. Additionally, several cis-elements in the promoter regions of GLPs were hormones, and stress-responsive and different families of miRNAs target StGLPs. Gene duplication under selection pressure also exhibited positive and purifying selections in StGLPs. In our results, the StGLP5 gene showed the highest expression in response to salt stress among all expressed StGLPs. Totally 19 StGLPs genes were expressed in response to heat stress. Moreover, three genes, StGLP30, StGLP17, and StGLP14, exhibited a relatively higher expression level in the potato after heat treatment. In total, 22 genes expressed in response to abscisic acid (ABA) treatment indicated that ABA performed an essential role in the plant defense or tolerance mechanism to environmental stress. RNA-Seq data validated by RT-qPCR also confirm that the StGLP5 gene showed maximum expression among selected genes under salt stress. Concisely, our results provide a platform for further functional exploration of the StGLPs against salt and heat stress conditions.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

et al. 2022

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“…The energy supply system plays a vital role in the whole process of seed germination. Transcriptome analysis during seed germination by [ 26 ] revealed that the EMP, TCA, and PPP are activated during germination [ 21 , 26 ]. Previous studies on the germination and emergence of aged sunflower and soybean seeds found that the germination rate and seedling establishment were closely related to fatty acid and sugar metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…This complex physiological process involves activities such as water absorption, storage degradation, seed respiration, transcription product (mRNA) synthesis, and mitochondrial repair and proliferation, all of which require an adequate energy supply [ 15 , 17 , 18 ]. The energy supply for seed germination and emergence comes from the catabolism of stored substances in the seed before the seedlings acquire photosynthetic capacity [ 19 , 20 , 21 ]. The mobilization of internal storage reserves during seed germination varies with the amount and type of storage substances.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism

Zhang

Wan

et al. 2022

Plants

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Starch, a substance stored in seeds, is the main source of energy for germination in sorghum seeds. However, as the seeds age, the catabolism of seed starch is affected, thereby seriously damaging germination ability. In this study, we aimed to understand how exogenous spermidine promoted germination in aged sorghum seed. Our phenotypic analysis indicated that exogenous spermidine not only significantly improved the germination rate, germination potential, germination index, and vigor index of aged seeds, but also increased the root and shoot length after germination. Further, physiological analysis showed that exogenous spermidine increased the content of soluble sugar by upregulating the activity of amylase and sucrose invertase. Exogenous spermidine also improved the activities of key enzymes in glycolysis, the tricarboxylic acid cycle, and the pentose phosphate pathway of aged sorghum seeds. Interestingly, exogenous spermidine protected the mitochondrial structure of aged seeds, which was consistent with the increase in the respiration rate and ATP content during seed germination. Moreover, qRT-PCR analysis revealed that exogenous spermidine induced the expression of key genes related to starch and sugar metabolism in aged sorghum seeds. In conclusion, our study demonstrated that exogenous spermidine promoted aged sorghum seed germination by regulating starch and sugar metabolism.

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“…In addition, the qRT-PCR amplification specificity was routinely checked with the melting curve. The data were observed through the 2 –ΔΔCt method ( Zaynab et al, 2018 ), while results were represented through relative gene expression level ( Zaynab et al, 2021d ). Through this analysis report, elongation factor 1 elongation factor 1 - α was considered housekeeping gene.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Expression Profiling of DUF221 Gene Family Provides New Insights Into Abiotic Stress Responses in Potato

et al. 2022

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The domain of the unknown function 221 proteins regulate several processes in plants, including development, growth, hormone transduction mechanism, and abiotic stress response. Therefore, a comprehensive analysis of the potato genome was conducted to identify the deafness-dystonia peptide (DDP) proteins’ role in potatoes. In the present study, we performed a genome-wide analysis of the potato domain of the unknown function 221 (DUF221) genes, including phylogenetic inferences, chromosomal locations, gene duplications, gene structures, and expression analysis. In our results, we identified 10 DDP genes in the potato genome. The phylogenetic analysis results indicated that StDDPs genes were distributed in all four clades, and clade IV was the largest clade. The gene duplication under selection pressure analysis indicated various positive and purifying selections in StDDP genes. The putative stu-miRNAs from different families targeting StDDPs were also predicted in the present study. Promoter regions of StDDP genes contain different cis-acting components involved in multiple stress responses, such as phytohormones and abiotic stress-responsive factors. The analysis of the tissue-specific expression profiling indicated the StDDPs gene expression in stem, root, and leaf tissues. We subsequently observed that StDDP4, StDDP5, and StDDP8 showed higher expressions in roots, stems, and leaves. StDDP5 exhibited high expression against heat stress response, and StDDP7 showed high transcript abundance against salt stress in potatoes. Under abscisic acid (ABA) and indole acetic acid (IAA) treatments, seven StDDP genes’ expressions indicated that ABA and IAA performed important roles in immunity response. The expression profiling and real-time qPCR of stems, roots, and leaves revealed StDDPs’ significant role in growth and development. These expression results of DDPs are primary functional analysis and present basic information for other economically important crops.

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Proteomics analysis of Cyclobalanopsis gilva provides new insights of low seed germination

Cited by 17 publications

References 59 publications

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism

Genome-Wide Identification and Expression Profiling of DUF221 Gene Family Provides New Insights Into Abiotic Stress Responses in Potato

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