Proteomics analysis mediated by quorum sensing luxS involved in oxidative stress in Cronobacter malonaticus

Ling, Na; Ou, Dexin; Shen, Yizhong; Zhang, Danfeng; Wang, Yaping; Tong, Lian; Ding, Yu; Wang, Juan; Yang, Xiaojuan; Zhang, Jumei; Wu, Qingping; Ye, Yingwang

doi:10.1016/j.lwt.2021.111576

Cited by 5 publications

(7 citation statements)

References 58 publications

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“…The inhibition rates of C. malonaticus WT and ΔluxS underlying hyperosmotic conditions C. malonaticus wild-type and luxS mutant were obtained from our laboratory. Details of the construction of ΔluxS strain are available in the previous works (Ling et al 2021a). Single colonies of C. malonaticus WT and the mutant strain were respectively picked to inoculate in liquid Luria-Bertani (LB) broth (Hopebio, Qingdao, China) and were grown overnight at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Details of the construction of Δ luxS strain are available in the previous works (Ling et al . 2021a). Single colonies of C. malonaticus WT and the mutant strain were respectively picked to inoculate in liquid Luria–Bertani (LB) broth (Hopebio, Qingdao, China) and were grown overnight at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The detailed procedures such as protein extraction, protein digestion, iTRAQ labelling, LC–MS/MS data collection, mass spectrometry data identification and quantification, and protein cluster and protein–protein interactions analysis were performed as described in our previous study (Ling et al . 2021a).…”

Section: Methodsmentioning

confidence: 99%

“…malonaticus WT and ΔluxS were treated with 5.5% NaCl, then the cultures were harvested for extraction of protein using a bacterial total protein kit (Sigma-Aldirch, Shanghai, China) according to the manual. The detailed procedures such as protein extraction, protein digestion, iTRAQ labelling, LC-MS/MS data collection, mass spectrometry data identification and quantification, and protein cluster and protein-protein interactions analysis were performed as described in our previous study (Ling et al 2021a).…”

Section: Proteomic Analysis Between Wt and δLuxs By Itraq In Osmotic ...mentioning

confidence: 99%

“…The nucleic acid purity and quantification were determined using a Nanodrop spectrophotometer (Thermo Scientific, Wilmington). PCR reactions, data analysis and the calculation of relative mRNA expression levels were performed as described in our previous study and the gyrB gene was used as the internal control (Ling et al 2021a).…”

Section: Relative Quantification Of Gene Expressionmentioning

confidence: 99%

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Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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Cronobacter malonaticus is a highly hyperosmotic‐resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum‐sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and ΔluxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe‐S cluster proteins and oxidoreductases, suggesting a potential cross‐tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS‐mediated osmotic tolerance to controlling C. malonaticus contamination.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Proteomic Analysis Between Wt and δLuxs By Itraq In Osmotic ...mentioning

confidence: 99%

Section: Relative Quantification Of Gene Expressionmentioning

confidence: 99%

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Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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LuxS-deficiency reduces persistence of Cronobacter to low-moisture but contributes to virulence after rehydration

Ling,

Li,

Wang

et al. 2023

Food Research International

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Insights into inactivation and response mechanisms of sublethal Listeria monocytogenes treated by cold plasma with joint transcriptomics and metabolomics

Pan

Sun

Cheng

et al. 2023

Journal of Applied Microbiology

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Aim The aim of the current study is to elucidate the inactivation and molecular response pattern of sublethal Listeria monocytogenes to cold plasma mediated two-pronged oxidative microenvironments from a high-throughput multi-omics perspective. Methods and results First joint transcriptomics and metabolomics analyses revealed that significantly expressed genes and metabolites were mainly involved in enhanced transmembrane transport and Fe2+/Cu+ efflux, amino acids limitation, cytoplasmic pH homeostasis, reconfiguration of central carbon metabolism flux and energy conservation strategy, which triggered the surge of intracellular endogenous oxidative stress and finally mediated bacterial ferroptosis and pathogenicity attenuation. Typical antioxidant systems such as the TrxR-Trx system and common antioxidant genes (e.g. sodA, katA, ahpC, trxA, spxA) were inhibited, and the more prominent antioxidant pathways include methionine metabolism, pentose phosphate pathway and glutathione metabolism as well as the DNA repair systems. Conclusions Therefore, our work confirmed from the transcriptional and metabolic as well as physiological levels that cold plasma-mediated intracellular oxidative stress induced big perturbations in pathways as a driving force for the inactivation and pathogenicity attenuation of L. monocytogenes.

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Proteomics analysis mediated by quorum sensing luxS involved in oxidative stress in Cronobacter malonaticus

Cited by 5 publications

References 58 publications

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

LuxS-deficiency reduces persistence of Cronobacter to low-moisture but contributes to virulence after rehydration

Insights into inactivation and response mechanisms of sublethal Listeria monocytogenes treated by cold plasma with joint transcriptomics and metabolomics

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