Proteomics analysis for the identification of three species of Thunnus

Abstract

The genus Thunnus comprises many species, some of higher quality and commercial value for their excellent organoleptic features, while others of lower quality and value. Consequently, these species are subjected to frequent fraudulent substitution. Increasing trade in fillet and minced fish makes the identification of external anatomical and morphological features of fish impossible. Proteomics was used for the identification of three Thunnus species. Muscle extracts were evaluated by both mono-and two-dimensional electrophoresis and mass spectrometric techniques. Preliminary results demonstrate that the tested species displays a high degree of polymorphism, making possible an accurate identification.Keywords Proteomics species identification . Thunnus genus . Two-dimensional electrophoresis Abbreviations 2DE two-dimensional electrophoresis IEF isoelectric focusing PCR polymerase chain reaction MALDI-TOF Matrix-assisted laser desorption/ionization-time of flight SDS-PAGE sodium dodecylsufate-polyacrylamide gel electrophoresis Vet Res Commun (2010) 34 (Suppl 1):S153-S155

The Discovery of Proteins Associated with Freshness of Coregonus Peled Muscle During Refrigerated Storage

“…Due to the role of endogenous protease and microorganism in muscles, the muscle protein was degraded, meanwhile the differential protein spots on the map showed varying degrees of change. The early stage of storage was generally dominated by the decomposition of endogenous proteases, and the decomposition of microbes produced at the later stage of storage was the main factor (Masniyom, Benjakul, & Visessanguan, 2005;Pepe et al, 2010;Verrez-Bagnis, Ladrat, Noelle, & Fleurence, 2002).…”

“…Two-dimensional gel electrophoresis (2-DE) in combination with mass spectrometry (MS) has been applied to characterize biochemistry of meat quality defects, and the proteomics data provided information related to the protein changes that exist in meat quality (Joseph, Nair, & Suman, 2015;Piras, Roncada, Rodrigues, Bonizzi, & Soggiu, 2016;Schilling et al, 2017). In fish research, proteomics has been successfully applied to fish authentication, allergen detection, microorganism contamination, and quality changes during storage and processing (Carrera et al, 2013;Fagerquist et al, 2009;Pepe et al, 2010), meanwhile it provides a new method in exploring potential protein changes that can be linked to meat freshness and quality defects. Guglielmetti JFDS-2019-0127 Submitted 1/26/2019, Accepted 4/9/2019 et al (2018) used 2-DE and LC-MS/MS to identify biomarkers that discriminate between fresh and frozen-thawed tissue samples of curled octopus (Eledone cirrhosa).…”

Proteomics in Food Science

“…The major advantage of the proposed method is the speed, due to the fast sample preparation step that avoids protein digestion. Recently, the comparison of the 2-DE sarcoplasmic protein patterns of three tuna species (Thunnus thynnus, Thunnus albacares, and Thunnus alalunga) revealed interspecies differences (Pepe et al, 2010). A protein with a M r of 70 kDa, present only in T. thynnus and identified by PMF as triose phosphate isomerase, was proposed as a potential specific marker for these species.…”

Interlaboratory Identification of Black Seabream (Spondyliosoma cantharus) as a Model Species on Basis of Polymerase Chain Reaction Targeting the Second Intron of the Parvalbumin Gene

“…) and modern proteomics‐based approaches (Pepe et al . ) are valuable methodologies for identification of fish authenticity. In recent years, molecular biological methods have constituted a parallel and very powerful tool (Bartlett and Davidson ; Rehbein et al .…”

“…With concern to the prospect of growing international trade and increasing number of potentially marketable species, it is worthwhile to conduct rapid and accurate analytical methods to distinguish fish without using morphological features (Etienne et al 1999;Bossier and Cooreman 2000). Protein electrophoresis (Graves et al 1988;Rehbein et al 1999a;Recio et al 2001;Smith et al 2001;Ataman et al 2006), immunochemical methods (Dominguez et al 1997) and modern proteomicsbased approaches (Pepe et al 2010) are valuable methodologies for identification of fish authenticity. In recent years, molecular biological methods have constituted a parallel and very powerful tool (Bartlett and Davidson 1991;Rehbein et al 1999b;Lin et al 2005;Hisar et al 2006;Choi and Hong 2007;Rehbein 2007;Espineira et al 2008aEspineira et al ,b, 2009Hubalkova et al 2008;Vinas and Tudela 2009;Tseng et al 2011;Aguilar et al 2012).…”