Proteomic Technology “Lens” for Epithelial-Mesenchymal Transition Process Identification in Oncology

Neagu, Monica; Constantin, Carolina; Bostan, Marinela; Căruntu, Constantin; Ignat, Simona; Dinescu, Sorina; Costache, Marieta

doi:10.1155/2019/3565970

Cited by 12 publications

(9 citation statements)

References 129 publications

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“…Using proteomics, new molecular pathway related to this process has been identified including MAPK, PI3K/AKT, WNT pathways and Akt/mTOR signaling pathway, thus providing critical insights into the development of novel therapeutic targets. 26,27 Recent studies have shown that EMT is associated with tumor cell migration and invasion, and cancer stem cell properties, including metastasis of glioma, human esophageal squamous cell carcinoma, and breast cancer. [28][29][30][31] And Recent evidence indicated that some lncRNAs could regulate the EMT process to promote tumor development, for example, LINC00460 silencing inhibited EMT in colon cancer via Upregulating miR-433-3p, 32 overexpression of GATA6 antisense RNA 1 (GATA6-AS1) Inhibited EMT through the Wnt/b-Catenin Signaling Pathway in GC, 33 CHRF promoted GC cell invasion and migration via regulating EMT.…”

Section: Discussionmentioning

confidence: 99%

<p>LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT</p>

Leng¹,

Liu²,

Wang³

et al. 2020

OTT

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Purpose: Gastric cancer (GC) is the fifth most common tumor in the world, and most patients with GC have a poor prognosis. This study aimed to explore the biological influence and mechanism of LINC01272 in GC. Materials and Methods: Using bioinformatic analyses, we investigated the expression of LINC01272 in TCGA database and predicted the biological functions and mechanism of LINC01272 in GC. Then, we detected the expression of LINC01272 in GC cell lines, GC tissues, and corresponding normal tissues using real-time polymerase chain reaction (RT-PCR). Finally, we explored the migration and invasion ability of LINC01272 by wound-healing and Transwell assays and examined the expression of epithelial-mesenchymal transition (EMT)related proteins through Western blotting. Results: We found that LINC01272 was upregulated in GC and was associated with GC staging and lymph node metastasis. The results of wound-healing and Transwell assays revealed that the LINC01272 was closely related to GC cell migration and invasion. LINC01272 knockdown inhibited the migration and invasion ability of GC cells by reducing the expression of EMT-related proteins. Overexpression of LINC01272 had the opposite effect. Conclusion: Together, our results showed that LINC01272 promoted GC metastasis ability by regulating the expression of EMT-related proteins and could serve as a potential diagnostic biomarker for GC.

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Section: Discussionmentioning

confidence: 99%

<p>LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT</p>

Leng¹,

Liu²,

Wang³

et al. 2020

OTT

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“…In the human body, MMPs are synthesized by numerous cellular types, such as fibroblasts, macrophages, endothelial cells, vascular smooth muscle, osteoblasts, etc. [ 12 , 13 ]. MMPs are grouped into several classes depending on the organization mode of their structural domains ( Table 1 ).…”

Section: Mmps As Molecular Promoters In Carcinogenesismentioning

confidence: 99%

“…The discovery of new substrates for MMPs offers novel perspectives on the role of MMPs in disease pathogenesis. MMPs are deeply involved in cell differentiation and proliferation, as well as in apoptosis, angiogenesis and the immune response [ 12 , 13 ].…”

Section: Mmps As Molecular Promoters In Carcinogenesismentioning

confidence: 99%

Current Perspectives on the Role of Matrix Metalloproteinases in the Pathogenesis of Basal Cell Carcinoma

et al. 2021

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Basal cell carcinoma (BCC) is the most common skin malignancy, which rarely metastasizes but has a great ability to infiltrate and invade the surrounding tissues. One of the molecular players involved in the metastatic process are matrix metalloproteinases (MMPs). MMPs are enzymes that can degrade various components of the extracellular matrix. In the skin, the expression of MMPs is increased in response to various stimuli, including ultraviolet (UV) radiation, one of the main factors involved in the development of BCC. By modulating various processes that are linked to tumor growth, such as invasion and angiogenesis, MMPs have been associated with UV-related carcinogenesis. The sources of MMPs are multiple, as they can be released by both neoplastic and tumor microenvironment cells. Inhibiting the action of MMPs could be a useful therapeutic option in BCC management. In this review that reunites the latest advances in this domain, we discuss the role of MMPs in the pathogenesis and evolution of BCC, as molecules involved in tumor aggressiveness and risk of recurrence, in order to offer a fresh and updated perspective on this field.

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“…Like in other solid tumors, the melanoma tumor microenvironment has an important role in the tumorigenesis dynamics (Neagu et al, 2019a). The tumor microenvironment consists of cancer-associated fibroblasts (CAFs), tumorassociated macrophages (TAMs), endothelial cells, immune cells, and a myriad of molecules with different origins.…”

Section: Tumor Microenvironment Mirna Expression In Cutaneous Melanomamentioning

confidence: 99%

miRNAs in the Diagnosis and Prognosis of Skin Cancer

Neagu

Constantin

Creţoiu

et al. 2020

Front. Cell Dev. Biol.

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Skin cancer is, at present, the most common type of malignancy in the Caucasian population. Its incidence has increased rapidly in the last decade for both melanoma and non-melanoma skin cancer. Differential expression profiles of microRNAs (miRNAs) have been reported for a variety of different cancers, including skin cancers. Since miRNAs' discovery as regulators of gene expression, their importance grew in the field of oncology. miRNAs can post-transcriptionally regulate gene expression, tumor initiation, development progression, and aggressiveness. Nowadays, these short regulatory RNAs are perceived as one of the epigenetic markers for the identification of new diagnostic and/or prognostic molecular markers. Moreover, as miRNAs can drive tumorigenesis, they might eventually represent new therapy targets. Some miRNAs are pleiotropic, such as miR-214, which was found deregulated in several other tumors besides skin cancers. Some others are specific for one or more skin cancer types, like miR-21 and miR-221 for cutaneous melanoma and cutaneous squamous carcinoma or miR-155 for melanoma and cutaneous lymphoma. The goal of this review was to summarize some of the main miRNA detection technologies that are used to evaluate miRNAs in tissues and body fluids. Furthermore, their quantification limits, conformity, and robustness are discussed. Aberrant miRNA expression is analyzed for cutaneous melanoma, cutaneous squamous cell carcinoma (CSCC), skin lymphomas, cutaneous lymphoma, and Merkel cell carcinoma (MCC). In this type of disease, miRNAs are described as potential biomarkers to diagnose early lesion and/or early metastatic disease. In the future, whether in tissue or circulating in body fluids, miRNAs will gain their place in skin cancer diagnosis, prognosis, and future therapeutic targets.

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Proteomic Technology “Lens” for Epithelial-Mesenchymal Transition Process Identification in Oncology

Cited by 12 publications

References 129 publications

<p>LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT</p>

<p>LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT</p>

Current Perspectives on the Role of Matrix Metalloproteinases in the Pathogenesis of Basal Cell Carcinoma

miRNAs in the Diagnosis and Prognosis of Skin Cancer

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