Proteomic surveillance of putative new autoantigens in thyroid orbitopathy

Cheng, Kai‐Chun; Hung, Chih‐Hsing; Cheng, Kai-Yuan; Chen, Kuo-Jen; Wu, Wen-Chuan; Suen, Jau‐Ling; Wu, Yu-Jen; Chang, Cheng-Hsien

doi:10.1136/bjophthalmol-2015-306634

Cited by 11 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NMU and LPAR5 may be the targets for thyroid cancer treatment, which are abnormally expressed in thyroid cancer tissues [36]. ADH1B [37] could be the target autoantigens in thyroid cancer and play potential clinical role. Kai-Chun et al [37] demonstrated that GNA14 involves in the regulation of the thyroid function and autoimmunity.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Network Analyses of Integrated Differentially Expressed Genes in Papillary Thyroid Carcinoma to Identify Characteristic Genes

Shang

Qian

Yuan

et al. 2019

Genes

View full text Add to dashboard Cite

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Identifying characteristic genes of PTC are of great importance to reveal its potential genetic mechanisms. In this paper, we proposed a framework, as well as a measure named Normalized Centrality Measure (NCM), to identify characteristic genes of PTC. The framework consisted of four steps. First, both up-regulated genes and down-regulated genes, collectively called differentially expressed genes (DEGs), were screened and integrated together from four datasets, that is, GSE3467, GSE3678, GSE33630, and GSE58545; second, an interaction network of DEGs was constructed, where each node represented a gene and each edge represented an interaction between linking nodes; third, both traditional measures and the NCM measure were used to analyze the topological properties of each node in the network. Compared with traditional measures, more genes related to PTC were identified by the NCM measure; fourth, by mining the high-density subgraphs of this network and performing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, several meaningful results were captured, most of which were demonstrated to be associated with PTC. The experimental results proved that this network framework and the NCM measure are useful for identifying more characteristic genes of PTC.

show abstract

Section: Resultsmentioning

confidence: 99%

“…ADH1B [37] could be the target autoantigens in thyroid cancer and play potential clinical role. Kai-Chun et al [37] demonstrated that GNA14 involves in the regulation of the thyroid function and autoimmunity. ALDH1A3 [37] may be the factor of mediating cancer stemness in thyroid cancer.…”

Section: Resultsmentioning

confidence: 99%

Network Analyses of Integrated Differentially Expressed Genes in Papillary Thyroid Carcinoma to Identify Characteristic Genes

Shang

Qian

Yuan

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…However, mild eye disease, with symptoms such as grittiness, pruritis and upper eyelid retraction occurs in 25% of patients with HT [5] and TRAb levels as measured by TSI/Thyretin are negative [26], indicating the possibility of a different eye disease.…”

Section: Discussionmentioning

confidence: 99%

Sight Threatening Thyroid Eye Disease Complicating Hashimoto’s Thyroiditis

Young¹,

Depczynski²,

Wall³

et al. 2017

Endocrinol Metab Syndr

View full text Add to dashboard Cite

A case of sight threatening thyroid eye disease (TED), arising in a 55 year old woman with primary hypothyroidism due to Hashimoto's thyroiditis (HT), is described. TSH receptor antibody (TSHRab) was positive as were orbital antibodies. Initial management consisted of methylprednisone intravenously followed by prednisone orally in a tapering dose, orbital irradiation and decompressive surgery. Visual acuity improved but 9 months later, there was evidence of relapse with features of compressive optic neuropathy, and exophthalmos had worsened. Prednisone dose was increased. At that time, TSHRab was negative. She underwent total thyroidectomy on the understanding that this was experimental therapy, surgery confirmed that thyroid pathology was solely TH without any evidence of Graves' disease. Prednisone was successively tapered without further relapse. Rehabilitative ocular muscle surgery is planned. Whether the improvement seen in TED can be attributed to effect of removal of thyroid antigens following thyroidectomy is purely speculative. Our case illustrates that relapsing sight threatening TED is part of spectrum of TED in HT and demonstrates that clinical features can be severe.

show abstract

“…It has been reported to be a useful biomarker for oral cancer (39). Previous study demonstrated AnxA2 participation in cell proliferation in orbital fat tissue of thyroid orbitopathy patients (45).…”

Section: Downregulation Of Anxa2 In Hepatocellular Carcinoma Cells Hamentioning

confidence: 99%

Downregulation of Calreticulin and Annexin A2 in Cholesteatoma by 2-DE Analysis

Hung

Chien

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Many factors are thought to be associated with the development of cholesteatoma, while the mechanisms of its formation remain unclear. This study aimed to identify the potential mechanisms of the proliferation and growth of cholesteatoma by analysis of the differential expressions of proteins in cholesteatoma and retroauricular skin tissue collected from patients. Methods: Comparative proteomics analyses using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), in addition to immunohistochemical analysis, were conducted to identify differentially-expressed proteins in cholesteatoma tissue as compared with retroauricular skin tissue. Western blotting was also employed to verify the expression patterns of the specific proteins identified by 2-DE and to measure the changes in potential modulators related to cholesteatoma proliferation and growth. Results: Calreticulin (CRT) and annexin A2 (AnxA2) were identified as being differentially-expressed in cholesteatoma by 2-DE and LC-MS/MS, the results of which were in agreement with the results of immunohistochemical analysis and western blotting. Downregulation of CRT and AnxA2 were observed in cholesteatoma. Conclusion: Our data suggested that CRT and AnxA2 were associated with cholesteatoma. We speculated that the reduced expression of CRT and the persistent inflammatory response play important roles in the epithelial proliferation of cholesteatoma.

show abstract

Proteomic surveillance of putative new autoantigens in thyroid orbitopathy

Abstract: We found that CA1 and ADH1B could attribute target autoantigens in this autoimmune disease. The high prevalence of these autoantibodies against CA1 and ADH1B in patients with TO and GD clarifies the potential clinical role for anti-CA1 and anti-ADH1B antibodies as biomarkers for GD and TO.

Cited by 11 publications

References 22 publications

Network Analyses of Integrated Differentially Expressed Genes in Papillary Thyroid Carcinoma to Identify Characteristic Genes

Network Analyses of Integrated Differentially Expressed Genes in Papillary Thyroid Carcinoma to Identify Characteristic Genes

Sight Threatening Thyroid Eye Disease Complicating Hashimoto’s Thyroiditis

Downregulation of Calreticulin and Annexin A2 in Cholesteatoma by 2-DE Analysis

Contact Info

Product

Resources

About