Proteomic Study Related to Vascular Connections in Watermelon Scions Grafted onto Bottle-Gourd Rootstock under Different Light Intensities

Muneer, Sowbiya; Ko, Chung Ho; Soundararajan, Prabhakaran; Manivnnan, Abinaya; Park, Yoo Gyeong; Jeong, Byoung Ryong

doi:10.1371/journal.pone.0120899

Cited by 22 publications

(31 citation statements)

References 53 publications

(61 reference statements)

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“…Protein synthesis plays an important role in plant cells with regard to many physiological processes in response to unfavorable conditions [ 46 – 47 ]. The expression of an abundant number of protein spots under Pi starvation which plays a crucial role in protein synthesis was observed to be up-regulated under Pi starvation.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.)

Muneer

Jeong

2015

PLoS ONE

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Phosphorus is a major nutrient acquired by plants via high-affinity inorganic phosphate (Pi) transporters. To determine the adaptation and homeostasis strategy to Pi starvation, we compared the proteome analysis of tomato leaves that were treated with and without Pi (as KH2PO4) for 10 days. Among 600 reproducible proteins on 2-DE gels 46 of them were differentially expressed. These proteins were involved in major metabolic pathways, including photosynthesis, transcriptional/translational regulations, carbohydrate/energy metabolism, protein synthesis, defense response, and other secondary metabolism. The results also showed that the reduction in photosynthetic pigments lowered P content under –Pi treatments. Furthermore, high-affinity Pi transporters (lePT1 and lePT2) expressed in higher amounts under –Pi treatments. Also, the accumulation of Pi transporters was observed highly in the epidermis and palisade parenchyma under +Pi treatments compared to –Pi treatments. Our data suggested that tomato plants developed reactive oxygen species (ROS) scavenging mechanisms to cope with low Pi content, including the up-regulation of proteins mostly involved in important metabolic pathways. Moreover, Pi-starved tomato plants increased their internal Pi utilization efficiency by increasing the Pi transporter genes and their rational localization. These results thus provide imperative information about how tomato plants respond to Pi starvation and its homeostasis.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.)

Muneer

Jeong

2015

PLoS ONE

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“…For 2D-PAGE analysis, protein extraction was carried out by following the procedure of Muneer et al [ 25 ]. In detail, leaf tissue (0.1 g) was homogenized in liquid nitrogen using a prechilled pestle and mortar.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 70 μ g of dissolved protein sample was separated by 2DE in the first dimension by isoelectric focusing on a 7 cm IPG strip ( pI 4–7) (GE Healthcare, UK) and the second dimension by SDS-PAGE on a Protean II unit (Bio-Rad Hercules, USA), according to methods given by Muneer et al [ 25 ]. The samples were rehydrated for 12 h (with 125 μ L rehydration buffer containing 70 μ g proteins) before focusing.…”

Section: Methodsmentioning

confidence: 99%

Silicon Mitigates Salinity Stress by Regulating the Physiology, Antioxidant Enzyme Activities, and Protein Expression inCapsicum annuum‘Bugwang’

Manivannan

Soundararajan

Muneer

et al. 2016

BioMed Research International

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Silicon- (Si-) induced salinity stress resistance was demonstrated at physiological and proteomic levels in Capsicum annuum for the first time. Seedlings of C. annuum were hydroponically treated with NaCl (50 mM) with or without Si (1.8 mM) for 15 days. The results illustrated that saline conditions significantly reduced plant growth and biomass and photosynthetic parameters and increased the electrolyte leakage potential, lipid peroxidation, and hydrogen peroxide level. However, supplementation of Si allowed the plants to recover from salinity stress by improving their physiology and photosynthesis. During salinity stress, Si prevented oxidative damage by increasing the activities of antioxidant enzymes. Furthermore, Si supplementation recovered the nutrient imbalance that had occurred during salinity stress. Additionally, proteomic analysis by two-dimensional gel electrophoresis (2DE) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) revealed that Si treatment upregulated the accumulation of proteins involved in several metabolic processes, particularly those associated with nucleotide binding and transferase activity. Moreover, Si modulated the expression of vital proteins involved in ubiquitin-mediated nucleosome pathway and carbohydrate metabolism. Overall, the results illustrate that Si application induced resistance against salinity stress in C. annuum by regulating the physiology, antioxidant metabolism, and protein expression.

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“…In previous studies, the optimal quality and intensity of supplementary lighting were determined [30][31][32][33][34]. Based on these previous studies, three durations of supplementary light (8,12,16 h/day) at 100 µmol•m −2 •s −1 PPFD using mixed LEDs (W 1 R 2 B 1 ) were tested to investigate the effects of different durations of supplementary lighting on the quality of grafted watermelon plug seedlings grown in a glasshouse after the graft healing period.…”

Section: Introductionmentioning

confidence: 99%

Effect of Supplementary Lighting Duration on Growth and Activity of Antioxidant Enzymes in Grafted Watermelon Seedlings

2020

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Insufficient exposure to light in the winter may result in a longer production periods and lower quality of seedlings in greenhouses for plug growers. Supplementary artificial lighting to plug seedlings may be one solution to this problem. The objective of this study was to assess the effects of the duration of the supplementary light on the growth and development of two watermelon cultivars, ‘Speed’ and ‘Sambok Honey’ grafted onto ‘RS-Dongjanggun’ bottle gourd rootstocks (Lagenaria siceraria Stanld). Seedlings were grown for 10 days in a glasshouse with an average daily natural light intensity of 340 μmol·m−2·s−1 photosynthetic photon flux density (PPFD) and daily supplementary lighting of 8, 12 or 16 h from mixed LEDs (W1R2B1, chip ratio of white:red:blue = 1:2:1) at a light intensity of 100 μmol·m−2·s−1 PPFD, a group without supplementary light was set as the control (CK). The culture environment in a glasshouse had 25/15 °C day/night temperatures, an 85 ± 5% relative humidity, and a natural photoperiod of 8 h. The results showed that all the growth and development parameters of seedlings grown with supplementary light were significantly greater than those without supplementary light (CK). The 12 and 16 h supplementary light resulted in greater growth and development parameters than the 8 h supplementary light did. The same trend was also found with the indexes that reflect the quality of the seedlings, such as the dry weight ratio of the shoot and root, total biomass, dry weight to height ratio of scions, and specific leaf weight. The 12 h and 16 h light supplements resulted in greater Dickson’s quality indexes compared to the 8 h supplementary light, and the 12 h supplementary light showed the greatest use efficiency of the supplementary light. 16 h of daily supplementary light significantly increased the H2O2 content and the antioxidant enzyme activities in seedlings compared to the other treatments. This indicated that 16 h of supplementary light led to certain stresses in watermelon seedlings. In conclusion, considering the energy consumption, 12 h of supplementary light was the most efficient in improving the quality of the two cultivars of grafted watermelon plug seedlings.

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Proteomic Study Related to Vascular Connections in Watermelon Scions Grafted onto Bottle-Gourd Rootstock under Different Light Intensities

Cited by 22 publications

References 53 publications

Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.)

Proteomic Analysis Provides New Insights in Phosphorus Homeostasis Subjected to Pi (Inorganic Phosphate) Starvation in Tomato Plants (Solanum lycopersicum L.)

Silicon Mitigates Salinity Stress by Regulating the Physiology, Antioxidant Enzyme Activities, and Protein Expression inCapsicum annuum‘Bugwang’

Effect of Supplementary Lighting Duration on Growth and Activity of Antioxidant Enzymes in Grafted Watermelon Seedlings

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