Proteomic study on sodium selenite-induced apoptosis of human cervical cancer HeLa cells

Fu, Liping; Liu, Qiong; Shen, Liming; Wang, Yong

doi:10.1016/j.jtemb.2011.06.001

Cited by 17 publications

(14 citation statements)

References 39 publications

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“…The effect of SSe on ROS in different cell lines is controversial. In harmony with our data, Chatzakos et al [ 41 ] and Fu et al [ 42 ] reported that low concentrations of SSe can regulate cellular redox levels, resist peroxidation, and protect against cancer, while higher concentrations exert an oxidative stress resulting in ROS-mediated apoptosis. Sarada et al [ 43 ] reported that addition of SSe to neuroblastoma cells in culture prior to hypoxia-induced ROS decreased the hypoxia-induced cell death.…”

Section: Discussionsupporting

confidence: 92%

The Combination of α‐Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?

Badr

Hafez

Agha

et al. 2016

Oxidative Medicine and Cellular Longevity

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α-Tocopheryl succinate (α-TOS), a mitochondria-targeting agent, induces apoptosis in malignant cells in vitro and in vivo. Selenite is a nutritional supplement that has been shown to stimulate apoptosis in cancer cells. This study was designed to investigate the cytotoxic effect of combined treatment of α-TOS and sodium selenite (SSe) in vitro and in vivo and to explore their effect on apoptosis and autophagy in breast cancer. The type of interaction between α-TOS and SSe was evaluated and levels of oxidative stress and apoptotic and autophagic markers were determined. SSe alone showed varying degrees of cytotoxicity on all the tested cell lines. Its combination with α-TOS was antagonistic in vitro in MCF7 and in vivo in mice bearing Ehrlich tumor compared to α-TOS-treated one. Combination of TOS with 2 μM of SSe increased the level of glutathione without changes in antiapoptotic markers Bcl-2 and Mcl-1 at 16 and 48 hrs. SSe decreased caspase 3 activity and protein level of caspases 7 and 9, while it increased autophagic markers beclin-1 and LC3B protein levels of MCF7 cells treated with α-TOS. In conclusion, SSe antagonizes α-TOS-induced apoptosis via inhibition of oxidative stress and promoting prosurvival machinery of autophagy.

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Section: Discussionsupporting

confidence: 92%

The Combination of α‐Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?

Badr

Hafez

Agha

et al. 2016

Oxidative Medicine and Cellular Longevity

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“…In 2003, for example, it was shown that the enzymatic activity of D-DT is detectable in the epidermis of the skin and is increased after UV irradiation [26]. Other studies employing proteomics demonstrated that D-DT is elevated in the rat liver [27] and in the urine in a model of carbon tetrachloride-induced hepatic fibrosis [28], and that its expression increased after selenite induced apoptosis in HeLa cancer cells [29]. Only in the last four years studies have appeared that focused on the biological role of D-DT [30–32].…”

Section: Introductionmentioning

confidence: 99%

D-dopachrome tautomerase (D-DT or MIF-2): Doubling the MIF cytokine family

et al. 2012

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D-dopachrome tautomerase (D-DT) is a newly described cytokine and a member of the macrophage migration inhibitory factor (MIF) protein superfamily. MIF is a broadly expressed pro-inflammatory cytokine that regulates both the innate and the adaptive immune response. MIF activates the MAP kinase cascade, modulates cell migration, and counter-acts the immunosuppressive effects of glucocorticoids. For many cell types, MIF also acts as an important survival or anti-apoptotic factor. Circulating MIF levels are elevated in the serum in different infectious and autoimmune diseases, and neutralization of the MIF protein via antibodies or small molecule antagonists improves the outcome in numerous animal models of human disease. Recently, a detailed investigation of the biological role of the closely homologous protein D-DT, which is encoded by a gene adjacent to MIF, revealed an overlapping functional spectrum with MIF. The D-DT protein also is present in most tissues and circulates in serum at similar concentrations as MIF. D-DT binds the MIF cell surface receptor complex, CD74/CD44, with high affinity and induces similar cell signaling and effector functions. Furthermore, an analysis of the signaling properties of the two proteins showed that they work cooperatively, and that neutralization of D-DT in vivo significantly decreases inflammation. In this review, we highlight the similarities and differences between MIF and D-DT, which we propose to designate “MIF-2” and discuss the implication of D-DT/MIF-2 expression in MIF-based therapies.

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“…The COF1 protein expression levels were upregulated by supplementation with Se (Table ; Fig. A) confirming the results of Fu and coworkers, who analyzed HeLa cells treated with 40 μmol/L selenite by a 2DE‐based analysis . In addition treatment of the prostate cancer cell line LNCaP with SeMet resulted in an upregulation of COF2, another ADF/cofilin family member , indicating that this protein family is regulated by Se.…”

Section: Discussionmentioning

confidence: 99%

Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

et al. 2017

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The essential trace element selenium (Se) is controversially discussed concerning its role in health and disease. Its various physiological functions are largely mediated by Se incorporation in the catalytic center of selenoproteins. In order to gain insights into the impact of Se deficiency and of supplementation with different Se compounds (selenite, selenate, selenomethionine) at defined concentrations (recommended, 150 μg/kg diet; excessive, 750 μg/kg diet) in murine colon tissues, a 20-week feeding experiment was performed followed by analysis of the protein expression pattern of colon tissue specimens by 2D-DIGE and MALDI-TOF MS. Using this approach, 24 protein spots were identified to be significantly regulated by the different Se compounds. These included the antioxidant enzyme peroxiredoxin-5 (PRDX5), proteins with binding capabilities, such as cofilin-1 (COF1), calmodulin, and annexin A2 (ANXA2), and proteins involved in catalytic processes, such as 6-phosphogluconate dehydrogenase (6PGD). Furthermore, the Se compounds demonstrated a differential impact on the expression of the identified proteins. Selected target structures were validated by qPCR and Western blot which mainly confirmed the proteomic profiling data. Thus, novel Se-regulated proteins in colon tissues have been identified, which expand our understanding of the physiologic role of Se in colon tissue.

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Proteomic study on sodium selenite-induced apoptosis of human cervical cancer HeLa cells

Cited by 17 publications

References 39 publications

The Combination of α‐Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?

The Combination of α‐Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?

D-dopachrome tautomerase (D-DT or MIF-2): Doubling the MIF cytokine family

Altered protein expression pattern in colon tissue of mice upon supplementation with distinct selenium compounds

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