Proteomic study of non-typable Haemophilus influenzae

Thorén, Kajsa; Gustafsson, Elisabet; Clevnert, Annica; Larsson, Thomas; Bergström, Jakob; Nilsson, Carol L.

doi:10.1016/s1570-0232(02)00560-3

Cited by 12 publications

(3 citation statements)

References 23 publications

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“…Furthermore, most of the protective antigens described to date were identified in this analysis, plus one novel protective antigen (Rodriguez-Ortega et al 2006). Other examples where proteomics has also been used to study bacterial pathogenesis and identify vaccine candidates include Streptococcus agalactiae (Hughes et al 2002;Hughes et al 2003) and H. influenzae (Langen et al 2000;Thoren et al 2002).…”

Section: Proteomicsmentioning

confidence: 90%

Design of New Vaccines in the Genomic and Post-genomic Era

Serino

Seib

Pizza

2012

Innovation in Vaccinology

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During the twentieth century, the introduction of vaccines changed the history of mankind, eliminating most of the childhood diseases that used to cause millions of deaths. However, where conventional vaccinology approaches failed, many new and emerging infectious diseases remain a threat to health worldwide. The advent of whole-genome sequencing and innovation in bioinformatic tools radically changed the way to design and develop new and improved vaccines, starting from the genomic information of a single bacterial or viral isolate, with a process named reverse vaccinology. As the genomic era progressed, reverse vaccinology has evolved in combination with different approaches, such as transcriptomics, metabolomics, structural genomics, proteomics, and immunomics, contributing to the design of new and universal vaccines. Furthermore, the genomic information of the host is increasingly being used to aid understanding of the human immune response to vaccines.

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Section: Proteomicsmentioning

confidence: 90%

Design of New Vaccines in the Genomic and Post-genomic Era

Serino

Seib

Pizza

2012

Innovation in Vaccinology

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“…OMP5, OMP1, OMP26, OMP6 and OMP2 were all among the most abundant proteins found in our lysate preparations. OMP6 is perhaps the most studied of these membrane proteins, yet previous proteomic analyses of NTHi lysates had failed to identify it as common using mass spectometry 28 . Undoubtedly, the sensitivity of mass spectometry, along with more robust bacterial protein databases, have increased our capability of comprehensively identifying proteins in biospecimens.…”

Section: Discussionmentioning

confidence: 99%

A proteomic characterization of NTHi lysates

Preciado¹,

Poley

Tsai³

et al. 2016

International Journal of Pediatric Otorhinolaryngology

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Background Non-typeable Haemophilus influenzae (NTHi) is a ubiquitous bacterial pathogen which accounts for a majority of human upper respiratory tract infections. Laboratory lysate preparations from this bacterium are commonly utilized to investigate the promulgation of inflammatory responses in respiratory and middle ear epithelium both in vivo and in vitro. We undertook an unbiased proteomics based analysis of NTHi lysate preps to: a) identify abundant bacterial proteins present in these lysates that could play a role in NTHi biological effects and b) determine the protein content variability in different lysate prep batches from the same NTHI strain. Study Design Proteomic analysis of laboratory NTHi lysate preparations from clinical strain 12. Methods NTHi lysates were denatured, gel-fractionated, digested by trypsin and the generated peptides were identified using a liquid chromatography tandem mass spectrometry (LC-MS/MS). Western blot analyses for the important proinflammatory enhancer, outer membrane protein 6 (OMP6), was performed to validate the MS findings. Luciferase assays for NF-kB activation were used to measure the pro-inflammatory biologic effects from each NTHi lysate preparation. Results The MS identified 793 unique NTHi proteins. Most common and abundant proteins found have been described to either contribute to biofilm formation, elude the innate immune system, or activate epithelial pro-inflammatory pathways such as Toll Like Receptor 2 (TLR-2) signaling and NF-kB transcription factor. Strong positive signal for OMP6 was found in each of the NTHi lysate preparations. Significant NF-kB promoter response activation as expected with NTHi stimulation over control was also noted for each NTHi lysate preparation. Conclusions Proteomics was a successful technique to broadly define the protein content of NTHi lysates. This is the first report of the proteome of NTHi lysates widely used in laboratories to study the biological effect of NTHi. Despite the variability of the protein composition from different preps, all the batches of NTHi lysates induced similar NFκB activation. LEVEL OF EVIDENCE NA

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“…The proteome map of H. influenzae is well‐advanced because that organism is easily cultivated and has been used to study the proteome under different growth conditions, including treatment with antibiotics that inhibit DNA, RNA, and protein synthesis (Evers et al, 2001; Gmuender et al, 2001). Another proteome study of that organism aimed at the identification of potential vaccine candidates (Thoren et al, 2002). The 2D‐gel proteome map of H. influenzae published in 2000 (Langen et al, 2000) displays approximately 500 identified proteins, representing about 30% of all predicted open reading frames.…”

Section: The Role Of Proteomics To Decipher the Bacterial Responsmentioning

confidence: 99%

Bacterial proteomics and its role in antibacterial drug discovery

Brötz‐Oesterhelt

Bandow

Labischinski

2004

Mass Spectrometry Reviews

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Gene-expression profiling technologies in general, and proteomic technologies in particular have proven extremely useful to study the physiological response of bacterial cells to various environmental stress conditions. Complex protein toolkits coordinated by sophisticated regulatory networks have evolved to accommodate bacterial survival under ever-present stress conditions such as varying temperatures, nutrient availability, or antibiotics produced by other microorganisms that compete for habitat. In the last decades, application of man-made antibacterial agents resulted in additional bacterial exposure to antibiotic stress. Whereas the targeted use of antibiotics has remarkably reduced human suffering from infectious diseases, the ever-increasing emergence of bacteria that are resistant to antibiotics has led to an urgent need for novel antibiotic strategies. The intent of this review is to present an overview of the major achievements of proteomic approaches to study adaptation networks that are crucial for bacterial survival with a special emphasis on the stress induced by antibiotic treatment. A further focus will be the review of the, so far few, published efforts to exploit the knowledge derived from bacterial proteomic studies directly for the antibacterial drug-discovery process.

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Proteomic study of non-typable Haemophilus influenzae

Cited by 12 publications

References 23 publications

Design of New Vaccines in the Genomic and Post-genomic Era

Design of New Vaccines in the Genomic and Post-genomic Era

A proteomic characterization of NTHi lysates

Bacterial proteomics and its role in antibacterial drug discovery

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