Proteomic Study for Responses to Cadmium Stress in Rice Seedlings

Ge, Cailin; Wang, Ze-gang; Wan, Dingzhen; Ding, Yan; Wang, Yulong; Shang, Qi; Luo, Sulan

doi:10.1016/s1672-6308(08)60054-2

Cited by 21 publications

(8 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using proteomics strategies, Ahsan et al (2007) identified 21 up-regulated proteins in rice seeds in response to 4-day Cd stress during germination process. Ge et al (2009) identified 13 differential proteins in rice roots and 12 differential proteins in rice leaves under 15-day Cd stress, Lee et al (2010) reported 36 differential proteins in rice roots under 24-h Cd stress, while Xue et al (2014) verified 47 differentially abundant proteins in rice grains at the filling stage under 3 weeks of Cd stress. Moreover, four Cd-induced proteins were reported to be related to tolerance to Cd stress in Shanyou 63 (Ge et al 2009).…”

Section: Introductionmentioning

confidence: 93%

“…Two rice (Oryza sativa L.) cultivars with different ability to avoid Cd stress, Shanyou 63 (Tolerant) and Nipponbare (Susceptible) (Ge et al 2009), were employed as materials. The germinated rice seedlings were grown in nutrient solution according to Li et al (2010), and all the plots were placed in a greenhouse at 28 ± 2°C during the day and 22 ± 2°C at night under natural light conditions.…”

Section: Plant Growth Condition and CD Treatmentmentioning

confidence: 99%

See 1 more Smart Citation

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

et al. 2015

View full text Add to dashboard Cite

Cadmium (Cd), a non-essential and toxic heavy metal element, is extremely harmful for rice growth, food safety and even public health. In this paper, two rice varieties, Shanyou 63 (Tolerant to Cd stress) and Nipponbare (Susceptible to Cd stress), were employed as materials to investigate the protein expression and protein-protein interaction in rice in response to different stages of Cd stress. The result showed that Cd accumulated in the root of both rice cultivars, but the Cd content in the root of Shanyou 63 was significantly lower than that in Nipponbare. Eight proteins were up-regulated in the two rice cultivars, but the expressing abundance of these eight proteins in Shanyou 63 was statistically higher than that in Nipponbare, indicating that the relative abundance of these eight proteins was positively related to the ability of rice cultivars to avoid Cd stress. According to Gene Ontology annotation, four of these eight proteins were involved in energy metabolism, while four participated in stress tolerance. Native-PAGE and LC-MS/MS methods revealed that four GSTs, one cysteine synthase and one protein disulfide isomerase formed a stable protein complex in Shanyou 63 root in response to 3-, 5-and 7-day Cd stresses, but this protein complex did not appear in Nipponbare root under Cd stress. Owing to the same domain, the four glutathione S-transferases (GSTs) and cysteine synthase had the potential to interact and compose a protein complex to detoxify Cd in Shanyou 63 rice plants. Our results provide new insight into the detoxification mechanism of rice plant in response to Cd stress and may prove to be useful for the future research.

show abstract

Section: Introductionmentioning

confidence: 93%

Section: Plant Growth Condition and CD Treatmentmentioning

confidence: 99%

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Besides, ferredoxin-NADP(H) oxidoreductase and peroxidase (POX) were up-regulated . Moreover, POX isoenzyme (a detoxification-related protein) was markedly induced in rice seedlings in response to 3-d and 7-d treatments of 0.1 mmol/L CdCl 2 (Ge et al, 2009). Thus, we can suspect that POX and photosynthesis-related proteins may play a role in providing metabolic energy and redox power for Cd detoxification.…”

Section: Metal Ionsmentioning

confidence: 99%

Proteomics characteristics of rice leaves in response to environmental factors

2010

View full text Add to dashboard Cite

Rice is an important food crop worldwide. Its productivity has been influenced by various abiotic and biotic factors including temperature, drought, salt, microbe, ozone, hormone and glyphosate. The responses of plants to stress are regulated by multiple signaling pathways, and the mechanisms of leaf growth and development in response to stress remain unclear to date. Recently, proteomics studies have provided new evidence for better understanding the mechanisms. The proteins in response to different stress conditions are mainly involved in photosynthesis, signal transduction, transcription, protein synthesis and destination, defense response, cytoskeleton, energy, cell wall and other metabolism. In addition, some stress type-specific proteins have been identified, such as small heat shock proteins under temperature stress, S-like RNase homolog and actin depolymerizing factor under drought stress, ascorbate peroxidase and lipid peroxidation under salt stress, probenazole-inducible protein and rice pathogenesis-related proteins under blast fungus. Many of the proteins including ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO), molecular chaperones, antioxidases and S-adenosylmethionine synthetase play very important roles in leaves. This paper reviews the proteomic characterization of rice leaves in response to various environmental factors.

show abstract

“…The identified proteins have been classified into 10 functional categories, including cell structure, cell growth/division, energy metabolism, disease/defense, intracellular traffic, metabolism, protein destination and storage, signal transduction, hypothetical functions, and unknown proteins [28]. Most rice leaf proteomes were obtained from green leaves [29,30,31,32,33,34,35] however, proteomes from purple anthocyanin-containing leaves have never been reported. The proteomic profiling of plants that accumulate anthocyanins, such as grapes [36], and more specifically the mesocarp of vine-ripened grapes have helped to elucidate the biochemical and physiological changes that occur during anthocyanin accumulation and have been of paramount importance in advancing the understanding of berry development and the ripening process [37].…”

Section: Introductionmentioning

confidence: 99%

A comparative proteomic study of white and black glutinous rice leaves

Phonsakhan

Kong-ngern

2015

Electronic Journal of Biotechnology

View full text Add to dashboard Cite

Background: Black glutinous rice contains remarkable levels of anthocyanins, which possess anti-oxidative properties and thus have health benefits. The accumulation of anthocyanins in grains of thirty black glutinous rice varieties was measured, and the results revealed that the accumulated anthocyanin content ranged from 0.262 to 2.539 mg/g. Black glutinous rice Br no. 19 was selected, and its leaf protein expression profile was compared with that of white glutinous rice RD 6 using 2D-PAGE, and the protein spots were then directly analyzed after proteolysis by LC-MS/MS. Results: The proteins from the leaves of the two rice varieties were separated using 2D-PAGE and silver stained, and the spots were analyzed using Image Master 2D Platinum version 5 software. The results showed that the protein profiles of these two rice cultivars were different, with at least six protein spots that were detected only in Br no. 19. In addition, seven protein spots accumulated at higher levels in Br no. 19 than in RD 6. Conclusion: The protein spot S1 (AP005098.4) is homologous to the Rc protein. Our results suggest that some of the proteins enriched in Br no. 19 may be involved in anthocyanin synthesis in the black glutinous rice.

show abstract

Proteomic Study for Responses to Cadmium Stress in Rice Seedlings

Cited by 21 publications

References 56 publications

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

Protein complex and proteomic profile in the roots of Oryza sativa L. in response to cadmium toxicity

Proteomics characteristics of rice leaves in response to environmental factors

A comparative proteomic study of white and black glutinous rice leaves

Contact Info

Product

Resources

About