Proteomic Studies of the Effects of Different Stress Conditions on Central Carbon Metabolism in Microorganisms

doi:10.4172/jpb.1000355

Cited by 8 publications

(8 citation statements)

References 93 publications

(96 reference statements)

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“…Generally, an overall decrease in TCA central metabolism is a common strategy for microorganisms to conserve energy under various environmental stresses (e.g., acid, hypoxia, ultraviolet light, and oxidative stress), and this strategy can also avoid the accompanying generation of intracellular ROS in downstream oxidative phosphorylation . Thus, compensatory upregulation of glycolysis and PPP is a survival advantage to fulfill energy requirements; in particular, a high glycolysis rate endows bacteria with rapid growth and speedy generation of ATP in stressful environments (as corroborated by the slight boost in ATP production and the fast growth of E GO cells relative to E.…”

Section: Resultsmentioning

confidence: 99%

Chronic Exposure to Low Concentration of Graphene Oxide Increases Bacterial Pathogenicity via the Envelope Stress Response

Zhang

2020

Environ. Sci. Technol.

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Graphene oxide (GO), which has diverse antimicrobial mechanisms, is a promising material to address antibiotic resistance. Considering the emergence of antibiotic tolerance/resistance due to prolonged exposure to sublethal antibiotics, it is imperative to assess the microbiological effects and related adaptive mechanisms under chronic exposure to sublethal levels of GO, which have rarely been explored. After repetitive exposure to 5 mg/L GO for 200 subcultures (400 days), evolved Escherichia coli (E. coli) cells (E GO) differed significantly from their ancestor cells according to transcriptomic and metabolomic analyses. Contact with GO surfaces transformed E. coli by activating the Cpx envelope stress response (ESR), resulting in more than twofold greater extracellular protease release and biofilm formation. The ESR also modulated the envelope structure and function via increases in membrane fluidity, permeation, and lipopolysaccharide content to fulfill growth requirements and combat envelope stress. As a consequence of metabolic adjustment, E GO cells showed advantages of surviving in an acidic and oxidative environment, which resembles the cytosol of host cells. With these adaptive features, E GO cells exhibited higher pathogenicity than ancestor E. coli cells as evidenced by increased bacterial invasion and intracellular survival and a more severe inflammatory response in macrophage cells. To conclude, we seek to raise awareness of the possible occurrence of microbial adaptation to antimicrobial nanomaterials, which may be implicated in cross-adaptation to harsh environments and eventually the prevalence of virulence.

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Section: Resultsmentioning

confidence: 99%

Chronic Exposure to Low Concentration of Graphene Oxide Increases Bacterial Pathogenicity via the Envelope Stress Response

Zhang

2020

Environ. Sci. Technol.

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“…The central carbon metabolism plays essential roles in a cell by providing energy and precursors for many biosynthetic pathways. Under stress conditions, the central carbon metabolism and other metabolic pathways become altered to provide the metabolites necessary to overcome the stress effects reviewed in [ 76 ]. For example, we observed increased amounts of the pentose phosphate pathway steps generating the reductant NADPH and nucleotide precursors for DNA repair [ 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

The Periplasmic Oxidoreductase DsbA Is Required for Virulence of the Phytopathogen Dickeya solani

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Bogucka

et al. 2022

IJMS

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In bacteria, the DsbA oxidoreductase is a crucial factor responsible for the introduction of disulfide bonds to extracytoplasmic proteins, which include important virulence factors. A lack of proper disulfide bonds frequently leads to instability and/or loss of protein function; therefore, improper disulfide bonding may lead to avirulent phenotypes. The importance of the DsbA function in phytopathogens has not been extensively studied yet. Dickeya solani is a bacterium from the Soft Rot Pectobacteriaceae family which is responsible for very high economic losses mainly in potato. In this work, we constructed a D. solani dsbA mutant and demonstrated that a lack of DsbA caused a loss of virulence. The mutant bacteria showed lower activities of secreted virulence determinants and were unable to develop disease symptoms in a potato plant. The SWATH-MS-based proteomic analysis revealed that the dsbA mutation led to multifaceted effects in the D. solani cells, including not only lower levels of secreted virulence factors, but also the induction of stress responses. Finally, the outer membrane barrier seemed to be disturbed by the mutation. Our results clearly demonstrate that the function played by the DsbA oxidoreductase is crucial for D. solani virulence, and a lack of DsbA significantly disturbs cellular physiology.

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“…25 Additionally, elevated expression of the glycolytic enzyme 2,3-bisphosphoglycerateindependent phosphoglycerate mutase observed for M. phaseolina was similar to that found for Pallisentis umbellatus, where both the enzymes and the glycolytic pathways were found to be induced under oxidative stressed condition during the initial sclerotia formation. 26 Phosphoglycerate kinase, a housekeeping enzyme of the glycolytic pathway reported to protect cells against oxidants, 27 was also in the list of proteins which showed an elevated expression. A proteome analysis of A. fumigatus, where 117 proteins were identified with an altered abundance in response to hypoxia, phosphoglycerate kinase was also shown to have increased activity.…”

Section: ■ Discussionmentioning

confidence: 99%

Proteome Analyses RevealMacrophomina phaseolina’s Survival Tools When Challenged byBurkholderia contaminansNZ

et al. 2020

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A phytopathogenic fungus, Macrophomina phaseolina, which infects a wide range of plants, is an important consideration in agronomy. A jute endophytic bacterium, Burkholderia contaminans NZ, was found to have a promising effect in controlling the fungus in in vitro culture conditions. Using the iTRAQ LC-MS/MS method for quantitative proteomics study, an analysis of the whole proteome of Macrophomina phaseolina with or without B. contaminans NZ challenge identified 2204 different proteins, of which 137 were found to have significant deviation in expression. Kyoto encyclopedia of genes and genomes pathway analysis identified most of the upregulated proteins to be functionally related to energy production (26.11%), as well as defense and stress response (23.45%), while there was significant downregulation in oxidative stress protection pathways (42.61%), growth and cell wall integrity (30.95%), and virulence (23.81%). Findings of this study suggest the development of a battle when the phytopathogen encounters the bacterium. B. contaminans NZ manages to arrest the growth of the fungus and decrease its pathogenicity, but the fungus apparently survives under "hibernating" conditions by upregulating its energy metabolism. This first ever proteomic study of M. phaseolina will go a long way in understanding and developing strategies for its effective control.

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Proteomic Studies of the Effects of Different Stress Conditions on Central Carbon Metabolism in Microorganisms

Cited by 8 publications

References 93 publications

Chronic Exposure to Low Concentration of Graphene Oxide Increases Bacterial Pathogenicity via the Envelope Stress Response

Chronic Exposure to Low Concentration of Graphene Oxide Increases Bacterial Pathogenicity via the Envelope Stress Response

The Periplasmic Oxidoreductase DsbA Is Required for Virulence of the Phytopathogen Dickeya solani

Proteome Analyses RevealMacrophomina phaseolina’s Survival Tools When Challenged byBurkholderia contaminansNZ

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