Proteomic screening of antioxidant effects exhibited by Radix Salvia miltiorrhiza aqueous extract in cultured rat aortic smooth muscle cells under homocysteine treatment

Effect of Salvia miltiorrhiza polysaccharides on boar spermatozoa during freezing–thawing

“…LDH, GOT and CAT are also important oxidative defense enzymes in the sperm antioxidant system, and a previous study showed that they were all decreased during sperm cryopreservation (Aitken and Baker, 2004;Sikka, 2004). The preset results show that SOD, LDH, GOT and CAT activities are greater in the groups supplemented with SMPs, compared to the control group (P < 0.05), which was in agreement with previous research (Matkowski et al, 2008;Hung et al, 2009) and infers that SMPs effectively protected sperm from the adverse effects of ROS. Hu et al (2014) found that added alginate in the extender could protect boar sperm from lipid peroxidation during spermatozoa freezing.…”

Inhibitory effects of Scutellaria baicalensis extract on hepatic stellate cells through inducing G2/M cell cycle arrest and activating ERK-dependent apoptosis via Bax and caspase pathway

“…A total of 1 × 10 5 cells were seeded in 24-well plates for 24 hours (h) and made quiescent by incubating in medium containing 0.2% FBS overnight. After treating with various concentrations (0, 10, 100, 250, 500, and 1000 g/mL) of SBEE for 72 h, isopropanol solution mixed with tetrazolium salt was added to the wells and incubated for additional 4 h at 37 • C (Hung et al, 2009). The optical density of the dissolved material was measured spectrophotometrically at 570 nm, and assays were performed in triplicate.…”

Systems Biology of Meridians, Acupoints, and Chinese Herbs in Disease

“…Moreover, Hung et al found that S. miltiorrhiza aqueous extract (SMAE) inhibited the proliferation of rat aortic smooth muscle cell line A10 under homocysteine (Hcy)-induced oxidative stress [87, 88]. Furthermore, the intracellular reactive oxygen species concentration significantly decreased in A10 cells after SMAE treatment.…”

“…Furthermore, the intracellular reactive oxygen species concentration significantly decreased in A10 cells after SMAE treatment. Using MALDI-TOF-MS, the researchers suggested that the SMAE-induced inhibition of growth in Hcy-stimulated A10 cells occurred via the PKC/MAPK-dependent pathway [87, 88]. …”