Proteomic response of Euglena gracilis to heavy metal exposure – Identification of key proteins involved in heavy metal tolerance and accumulation

Khatiwada, Bishal; Hasan, Mafruha T.; Sun, Angela; Kamath, Karthik Shantharam; Mirzaei, Mehdi; Sunna, Anwar; Nevalainen, Helena

doi:10.1016/j.algal.2019.101764

Cited by 69 publications

(59 citation statements)

References 80 publications

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“…The ability of the E. gracilis Zm-strain to withstand heavy metal exposure was determined by the MIC assay as previously described [28]. Briefly, the cultures were subjected to different concentrations of metal: Hg (5-90 ppm), Pb (500-10,000 ppm) and Cd (50-1000 ppm).…”

Section: Minimum Inhibitory Concentration (Mic) Assaymentioning

confidence: 99%

“…Cells were grown in a GNY medium [29] supplemented with either Cd (200 ppm), Pb (500 ppm) and Hg (50 ppm), selected from the MIC study. Samples were collected at different time points (day 2, 4, 6 and 7) of cultivation and heavy metal accumulation was determined as described previously [28].…”

Section: Determination Of Heavy Metal Bioaccumulationmentioning

confidence: 99%

“…This study was conducted to explore the role of chloroplasts in the accumulation of heavy metals (Cd, Hg and Pb) in E. gracilis. The MIC and the maximum ability to accumulate heavy metals were compared between the previously assessed wild-type Z-strain [28] that can develop mature chloroplasts and a variant strain (Zm) that was unable to develop chloroplasts. Sequential window acquisition of all theoretical fragment-ion spectra-mass spectrometry (SWATH-MS) was performed to analyze differences in the proteomes between the two strains.…”

Section: Introductionmentioning

confidence: 99%

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Probing the Role of the Chloroplasts in Heavy Metal Tolerance and Accumulation in Euglena gracilis

et al. 2020

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The E. gracilis Zm-strain lacking chloroplasts, characterized in this study, was compared with the earlier assessed wild type Z-strain to explore the role of chloroplasts in heavy metal accumulation and tolerance. Comparison of the minimum inhibitory concentration (MIC) values indicated that both strains tolerated similar concentrations of mercury (Hg) and lead (Pb), but cadmium (Cd) tolerance of the Z-strain was twice that of the Zm-strain. The ability of the Zm-strain to accumulate Hg was higher compared to the Z-strain, indicating the existence of a Hg transportation and accumulation mechanism not depending on the presence of chloroplasts. Transmission electron microscopy (TEM) showed maximum accumulation of Hg in the cytosol of the Zm-strain and highest accumulation of Cd in the chloroplasts of the Z-strain indicating a difference in the ability of the two strains to deposit heavy metals in the cell. The highly abundant heavy metal transporter MTP2 in the Z-strain may have a role in Cd transportation to the chloroplasts. A multidrug resistance-associated protein highly increased in abundance in the Zm-strain could be a potential Hg transporter to either cytosol or mitochondria. Overall, the chloroplasts appear to have major role in the tolerance and accumulation of Cd in E. gracilis.

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Section: Minimum Inhibitory Concentration (Mic) Assaymentioning

confidence: 99%

Section: Determination Of Heavy Metal Bioaccumulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Probing the Role of the Chloroplasts in Heavy Metal Tolerance and Accumulation in Euglena gracilis

et al. 2020

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“…The heavy metals can attach to the metal-binding sites of proteins and stimulate the synthesis of related enzymes to regulate the synthesis and transport of related ingredients. 32 The response changes of the proteins in mycelia were explored.…”

Section: Response Changes Of Related Active Ingredients Under Pb 2+ Smentioning

confidence: 99%

Tolerance mechanism of Trichoderma asperellum to Pb²⁺: response changes of related active ingredients under Pb²⁺ stress

Sun

Hao

et al. 2020

RSC Adv.

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“…For example, genomic analyses of green microalgae, such as Chlamydomonas reinhardtii, and red microalgae, such as Cyanidioschyzon merolae indicate that there are a wide variety of metal transporter pathways that are conserved between microalgae and higher plants alongside other eukaryotes such as yeast (Hanikenne et al 2005;Blaby-Haas and Merchant 2012). The identification of putative algal metal transporter genes has been complemented by other genome-scale analyses such as by proteomic and transcriptomic methods Beauvais-Flück et al 2019;Puente-Sánchez et al 2018;Khatiwada et al 2020), and subsequent functional characterisation of individual metal transporters (Ibuot et al 2017;Tsednee et al 2019;Chang et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Multi-genomic analysis of the Cation Diffusion Facilitator transporters from algae

Ibuot

Dean

Pittman³

2019

Preprint

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Metal transport processes are relatively poorly understood in algae in comparison to higher plants and other eukaryotes. A screen of genomes from 33 taxonomically diverse algal species was conducted to identify members of the Cation Diffusion Facilitator (CDF) family of metal ion transporter. All algal genomes contained at least one CDF gene with four species having >10 CDF genes (median of 5 genes per genome), further confirming that this is a ubiquitous gene family. Phylogenetic analysis suggested a CDF gene organisation of five groups, which includes Zn-CDF, Fe/Zn-CDF and Mn-CDF groups, consistent with previous phylogenetic analyses, and two functionally undefined groups. One of these undefined groups was algal specific although excluded chlorophyte and rhodophyte sequences. The majority of sequences (22 out of 26 sequences) from this group had a putative ion binding site motif within transmembrane domain 2 and 5 that was distinct from other CDF proteins, such that alanine or serine replaced the conserved histidine residue.The phylogenetic grouping was supported by sequence cluster analysis. Yeast heterologous expression of CDF proteins from Chlamydomonas reinhardtii indicated Zn 2+ and Co 2+ transport function by CrMTP1, and Mn 2+ transport function by CrMTP2, CrMTP3 and CrMTP4, which validated the phylogenetic prediction. However, the Mn-CDF protein CrMTP3 was also able to provide zinc and cobalt tolerance to the Zn-and Co-sensitive zrc1cot1 yeast strain. There is wide diversity of CDF transporters within the algae lineage, and some of these genes may be attractive targets for future applications of metal content engineering in plants or microorganisms. broad taxonomic lineage. Furthermore, a characterisation of additional members of the C. reinhardtii MTP gene family was carried out by using yeast heterologous expression to infer the metal substrates and determine metal tolerance functions. Materials and Methods Bioinformatic analysisThirty three algal species genomes were screened from one Charophyta: Klebsormidium nitens; 13 Chlorophyta: Bathycoccus prasinos RCC1105, Botryococcus braunii,

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Proteomic response of Euglena gracilis to heavy metal exposure – Identification of key proteins involved in heavy metal tolerance and accumulation

Cited by 69 publications

References 80 publications

Probing the Role of the Chloroplasts in Heavy Metal Tolerance and Accumulation in Euglena gracilis

Probing the Role of the Chloroplasts in Heavy Metal Tolerance and Accumulation in Euglena gracilis

Tolerance mechanism of Trichoderma asperellum to Pb²⁺: response changes of related active ingredients under Pb²⁺ stress

Multi-genomic analysis of the Cation Diffusion Facilitator transporters from algae

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Proteomic response of Euglena gracilis to heavy metal exposure – Identification of key proteins involved in heavy metal tolerance and accumulation

Cited by 69 publications

References 80 publications

Probing the Role of the Chloroplasts in Heavy Metal Tolerance and Accumulation in Euglena gracilis

Probing the Role of the Chloroplasts in Heavy Metal Tolerance and Accumulation in Euglena gracilis

Tolerance mechanism of Trichoderma asperellum to Pb2+: response changes of related active ingredients under Pb2+ stress

Multi-genomic analysis of the Cation Diffusion Facilitator transporters from algae

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Tolerance mechanism of Trichoderma asperellum to Pb²⁺: response changes of related active ingredients under Pb²⁺ stress