Proteomic response of Escherichia coli to the alkaloid extract of Papaver polychaetum

Abstract

The cellular response of Escherichia coli exposed to alkaloids extracted from a biennial endemic plant, Papaver polychaetum, was explored using proteome analysis. Following determination of the minimum inhibitory concentration of the berberine-containing plant extract as 1,250 μg/mL, E. coli cells were grown in the presence of 750 μg/mL extract. The response of the bacteria to the extract, with berberine found as the major alkaloid, was analyzed on two-dimensional gels. The differentially expressed proteins in the presence of 750 μg/mL extract were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. These proteins included those that play vital roles for maintenance such as protein synthesis (elongation factor-Ts), transport (oligopeptide-binding protein A, uncharacterized amino-acid ABC transporter ATP binding protein YECC), energy metabolism (alpha-subunit of ATP synthase, pyridine nucleotide transhydrogenase STHA) and regulation. These results provide clues for understanding the mechanism of the alkaloid extract-induced stress and cytotoxicity on E. coli. The altered proteins can serve as potential targets for development of innovative therapeutic agents.

Plant-Derived Products with Therapeutic Potential against Gastrointestinal Bacteria

“…Interestingly, this concept can be extended to the study of the relationship between whole metabolomes of natural products and their biological effects [ 134 ]. Different studies suggest that various enzymes, metabolic pathways, cell membrane or cell wall transport proteins and chaperones may also serve as potent targets for berberine, a natural product isolated from medicinal plants [ 66 , 138 , 139 , 140 , 141 ]. In a metabolomics study which combined principal component analysis, the antibacterial activity of traditional Chinese medicine Aquilegia oxysepala extract on Staphylococcus aureus and its main chemical components (genkwanin, apigenin, maguoflorine, and berberine), which are used for treating GI infections, were examined in comparison with nine antibiotics with known mechanisms of action [ 142 ].…”

Investigation of the in vivo interaction between β-lactamaseand its inhibitor protein

“…The β-lactamase band was excised from the Coomassie Brilliant Blue stained gel and mass spectrometric analysis of the protein was performed as previously described (Shevchenko et al, 2007;Ozbalci et al, 2010).…”