Proteomic researches for lignocellulose-degrading enzymes: A mini-review

Guo, Hongliang; Wang, Xiao‐Dong; Lee, Dong Hoon

doi:10.1016/j.biortech.2018.05.101

Cited by 38 publications

(16 citation statements)

References 134 publications

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“…Their presence could be evaluated in future work by performing secretome or transcriptome analyses. Both are effectively used in a search for the new enzymes responsible for degradation of recalcitrant compounds by ligninolytic fungi (Guo et al, ; Zhang et al, ).…”

Section: Discussionmentioning

confidence: 99%

The Role of Ligninolytic Enzymes Laccase and a Versatile Peroxidase of the White‐Rot Fungus Lentinus tigrinus in Biotransformation of Soil Humic Matter: Comparative In Vivo Study

2018

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Soil organic matter (SOM) turnover by ligninolytic fungi is a large-scale process that controls organic carbon geochemistry in terrestrial ecosystems. However, the role of certain oxidative enzymes (e.g., laccase) in humus degradation remains unclear, as well as the molecular structure and recalcitrance of SOM components. In order to address these questions, the degradation of forest soil humic acid (HA) in the presence of laccase and a versatile peroxidase (VP) has been studied in the liquid culture of Lentinus tigrinus. Contrary to the evolving views on humus structure, we have found that alkali-extractable and acid-insoluble constituents of SOM (HA) contain true macromolecular components, stable in the presence of 0.1% sodium dodecyl sulfate but degradable/resynthesizable by oxidative enzymes acting on covalent linkages. The HA degradation in the presence of laccase (high N medium) occurs at slower initial rate than in the presence of VP (low N medium). However, each of the enzymes caused about 60% color loss and almost complete degradation of HA into smaller molecules (gel-filtration data) within 2 weeks of cultivation. Depolymerization of HA in the culture liquid in the presence of laccase was accompanied by polymerization of degradation products on mycelium. Our results show that (1) humus macromolecules are not stable to oxidative enzymes once desorbed from the mineral phase, (2) laccase of Lentinus tigrinus is comparable by its degradation potential to VP, and (3) interfacial secondary synthesis reactions occur during humus decay in the presence of laccase. Our results highlight the important role of laccases in SOM sequestration in soils.

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Section: Discussionmentioning

confidence: 99%

The Role of Ligninolytic Enzymes Laccase and a Versatile Peroxidase of the White‐Rot Fungus Lentinus tigrinus in Biotransformation of Soil Humic Matter: Comparative In Vivo Study

2018

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“…Thus, facilitating hydrolysis and subsequent polysaccharide conversion is critical to improve biofuels production feasibility and competitivity against other fuel alternatives. Most of the proteomics studies on the degradation of lignocellulosic biomass to obtain value-added products has been primarily employed to determine hydrolytic enzymes, particularly, the extracellular enzymes secreted by microorganisms (Figure 2C), which together are known as the secretome [42]. The simple sugars obtained after the hydrolysis of polysaccharides by secretome enzymes are readily utilized as a carbon source for biofuel [43].…”

Section: Proteins Involved In Lignocellulose Utilizationmentioning

confidence: 99%

Bioconversion of Lignocellulosic Biomass into Value Added Products under Anaerobic Conditions: Insight into Proteomic Studies

Vélez‐Mercado

Talavera-Caro

Escobedo-Uribe

et al. 2021

IJMS

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Production of biofuels and other value-added products from lignocellulose breakdown requires the coordinated metabolic activity of varied microorganisms. The increasing global demand for biofuels encourages the development and optimization of production strategies. Optimization in turn requires a thorough understanding of the microbial mechanisms and metabolic pathways behind the formation of each product of interest. Hydrolysis of lignocellulosic biomass is a bottleneck in its industrial use and often affects yield efficiency. The accessibility of the biomass to the microorganisms is the key to the release of sugars that are then taken up as substrates and subsequently transformed into the desired products. While the effects of different metabolic intermediates in the overall production of biofuel and other relevant products have been studied, the role of proteins and their activity under anaerobic conditions has not been widely explored. Shifts in enzyme production may inform the state of the microorganisms involved; thus, acquiring insights into the protein production and enzyme activity could be an effective resource to optimize production strategies. The application of proteomic analysis is currently a promising strategy in this area. This review deals on the aspects of enzymes and proteomics of bioprocesses of biofuels production using lignocellulosic biomass as substrate.

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“…Proteomic approaches are successfully used to identify the carbohydrate-active enzymes (CAZymes) from the secretome of biomass deconstructing microbes [ 116 ]. Additionally, proteomic analyses provide the quantitative expression of enzymes in response to the available substrates.…”

Section: Introductionmentioning

confidence: 99%

Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review

Sethupathy

Morales

et al. 2021

Biotechnol Biofuels

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The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem functioning. Therefore, the use of microbes is an attractive way to transform biomass to produce clean energy and high-value compounds. The microbial degradation of lignocelluloses is a complex process which is dependent upon multiple secreted enzymes and their synergistic activities. The availability of the cutting edge proteomics and highly sensitive mass spectrometry tools make possible for researchers to probe the secretome of microbes and microbial consortia grown on different lignocelluloses for the identification of hydrolytic enzymes of industrial interest and their substrate-dependent expression. This review summarizes the role of secretomics in identifying enzymes involved in lignocelluloses deconstruction, the development of enzyme cocktails and the construction of synthetic microbial consortia for biomass valorization, providing our perspectives to address the current challenges.

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Proteomic researches for lignocellulose-degrading enzymes: A mini-review

Cited by 38 publications

References 134 publications

The Role of Ligninolytic Enzymes Laccase and a Versatile Peroxidase of the White‐Rot Fungus Lentinus tigrinus in Biotransformation of Soil Humic Matter: Comparative In Vivo Study

The Role of Ligninolytic Enzymes Laccase and a Versatile Peroxidase of the White‐Rot Fungus Lentinus tigrinus in Biotransformation of Soil Humic Matter: Comparative In Vivo Study

Bioconversion of Lignocellulosic Biomass into Value Added Products under Anaerobic Conditions: Insight into Proteomic Studies

Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review

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