Proteomic Research of Extracellular Vesicles in Clinical Biofluid

Fan, Shipan; Poetsch, Ansgar

doi:10.3390/proteomes11020018

Cited by 4 publications

(2 citation statements)

References 211 publications

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“…While effective, it is important to note that no antibody column can deplete all high-abundance proteins, making this method insufficient for detecting trace proteins in serum. To address this limitation, one approach for studying trace proteins in serum and plasma, avoiding interference from highly abundant proteins, involves collecting and analyzing extracellular vesicles via liquid chromatography-MS (LC-MS) [10][11][12]. In addition, the use of chemical nanoparticle cocktails for capturing trace serum and plasma proteins has recently gained attention [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Serum Purified Using Solanum tuberosum and Lycopersicon esculentum Lectins

Nakajima,

Konno,

Miyashita

et al. 2024

IJMS

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Serum and plasma exhibit a broad dynamic range of protein concentrations, posing challenges for proteome analysis. Various technologies have been developed to reduce this complexity, including high-abundance depletion methods utilizing antibody columns, extracellular vesicle enrichment techniques, and trace protein enrichment using nanobead cocktails. Here, we employed lectins to address this, thereby extending the scope of biomarker discovery in serum or plasma using a novel approach. We enriched serum proteins using 37 different lectins and subjected them to LC–MS/MS analysis with data-independent acquisition. Solanum tuberosum lectin (STL) and Lycopersicon esculentum lectin (LEL) enabled the detection of more serum proteins than the other lectins. STL and LEL bind to N-acetylglucosamine oligomers, emphasizing the significance of capturing these oligomer-binding proteins when analyzing serum trace proteins. Combining STL and LEL proved more effective than using them separately, allowing us to identify over 3000 proteins from serum through single-shot proteome analysis. We applied the STL/LEL trace-protein enrichment method to the sera of systemic lupus erythematosus model mice. This revealed differences in >1300 proteins between the systemic lupus erythematosus model and control mouse sera, underscoring the utility of this method for biomarker discovery.

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Section: Introductionmentioning

confidence: 99%

Proteome Analysis of Serum Purified Using Solanum tuberosum and Lycopersicon esculentum Lectins

Nakajima,

Konno,

Miyashita

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…In recent years, the study of EVs has been greatly facilitated by the development of new and improved methods. These methods enable their detection and purification from a variety of biological fluids, as well as their imaging, size measurement, and content analysis through lipidomics, proteomics, and genomics studies [ 6 , 7 ]. It is worth highlighting that major technological advances now enable specific analysis at the level of single vesicles.…”

Section: Introductionmentioning

confidence: 99%

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

El Safadi,

Mokhtari,

Krejbich

et al. 2024

Vaccines

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Exosomes are small subtypes of extracellular vesicles (EVs) naturally released by different types of cells into their environment. Their physiological roles appear to be multiple, yet many aspects of their biological activities remain to be understood. These vesicles can transport and deliver a variety of cargoes and may serve as unconventional secretory vesicles. Thus, they play a crucial role as important vectors for intercellular communication and the maintenance of homeostasis. Exosome production and content can vary under several stresses or modifications in the cell microenvironment, influencing cellular responses and stimulating immunity. During infectious processes, exosomes are described as double-edged swords, displaying both beneficial and detrimental effects. Owing to their tractability, the analysis of EVs from multiple biofluids has become a booming tool for monitoring various pathologies, from infectious to cancerous origins. In this review, we present an overview of exosome features and discuss their particular and ambiguous functions in infectious contexts. We then focus on their properties as diagnostic or therapeutic tools. In this regard, we explore the capacity of exosomes to vectorize immunogenic viral antigens and their function in mounting adaptive immune responses. As exosomes provide interesting platforms for antigen presentation, we further review the available data on exosome engineering, which enables peptides of interest to be exposed at their surface. In the light of all these data, exosomes are emerging as promising avenues for vaccine strategies.

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Eosinophil-derived extracellular vesicles: isolation and classification techniques and implications for disease pathophysiology

Rodrigo-Muñoz,

Gil-Martínez,

Naharro-González

et al. 2024

Journal of Leukocyte Biology

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Eosinophils are leukocytes characterized by their ability to release granule content that is highly rich in enzymes and proteins. Besides the anti-helminthic, bactericidal, and antiviral properties of eosinophils and their secretory granules, these also play a prominent role in the pathophysiology of diseases like asthma, eosinophilic esophagitis, and other hypereosinophilic conditions by causing tissue damage and airway hyperresponsiveness. Although this cell was first recognized mainly for its capacity to release granule content, nowadays other capabilities such as cytokine secretion have been linked to its physiology, and research has found that eosinophils are not only involved in innate immunity, but also as orchestrators of immune responses. Nearly 10 years ago, eosinophil-derived extracellular vesicles (EVs) were first described; since then, the EV field has grown exponentially, revealing their vital roles in intracellular communication. In this review, we synthesize current knowledge on eosinophil-derived EVs, beginning with a description of what they are and what makes them important regulators of disease, followed by an account of the methodologies used to isolate and characterize EVs. We also summarize current understanding of eosinophil-derived vesicles functionality, especially in asthma, the disease in which eosinophil-derived EVs have been most widely studied, describing how they modulate the role of eosinophils themselves (through autocrine signaling) and the way they affect airway structural cells and airway remodeling. Deeper understanding of this cell type could lead to novel research in eosinophil biology, its role in other diseases, and possible use of eosinophil-derived EVs as therapeutic targets.

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Proteomic Research of Extracellular Vesicles in Clinical Biofluid

Cited by 4 publications

References 211 publications

Proteome Analysis of Serum Purified Using Solanum tuberosum and Lycopersicon esculentum Lectins

Proteome Analysis of Serum Purified Using Solanum tuberosum and Lycopersicon esculentum Lectins

Exosome-Mediated Antigen Delivery: Unveiling Novel Strategies in Viral Infection Control and Vaccine Design

Eosinophil-derived extracellular vesicles: isolation and classification techniques and implications for disease pathophysiology

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