Proteomic profiling reveals that rabies virus infection results in differential expression of host proteins involved in ion homeostasis and synaptic physiology in the central nervous system

Dhingra, Vikas; Li, Xiaqing; Liu, Yuru; Fu, Zhen F.

doi:10.1080/13550280601178226

Cited by 63 publications

(50 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No evidence of apoptosis has been observed in postmortem brain tissues from fatal human rabies cases . Proteomic analysis indicated that only attenuated strains stimulate the expression of proapoptotic proteins; proteins that play a critical role in inducing apoptosis (Dhingra et al, 2007). It is believed that the expression level of viral glycoprotein is a critical factor for inducing apoptosis as attenuated strains produce a greater amount of glycoprotein in comparison with pathogenic strains (Faber et al, 2002 andPrehaud et al, 2003).…”

Section: Host Immune Responsementioning

confidence: 99%

Mitochondrial dysfunction in rabies virus infection of neurons

et al. 2013

View full text Add to dashboard Cite

Section: Host Immune Responsementioning

confidence: 99%

Mitochondrial dysfunction in rabies virus infection of neurons

et al. 2013

View full text Add to dashboard Cite

“…In contrast, only about 1.4% of genes became upregulated, including genes involved in regulation of cell metabolism, protein synthesis, and growth and differentiation. Using proteomic profiling on brain homogenates in mice infected with the street rabies virus, Dhingra et al 36 found that the expression of proteins involved in ion homeostasis was altered (H + ATPase and Na + /K + ATPase were upregulated and Ca 2+ ATPase were downregulated), and there was downregulation of proteins relevant to synaptic physiology, which are involved in docking and fusion of synaptic vesicles to the presynaptic membrane. These abnormalities could all a play a role in neuronal dysfunction.…”

Section: Mechanisms For Neuronal Dysfunction and Deathmentioning

confidence: 99%

Update on rabies

Jackson¹

2011

RRTM

View full text Add to dashboard Cite

Human rabies is almost invariably fatal, and globally it remains an important public health problem. Our knowledge of rabies pathogenesis has been learned mainly from studies performed in experimental animal models, and a number of unresolved issues remain. In contrast with the neural pathway of spread, there is still no credible evidence that hematogenous spread of rabies virus to the central nervous system plays a significant role in rabies pathogenesis. Although neuronal dysfunction has been thought to explain the neurological disease in rabies, recent evidence indicates that structural changes involving neuronal processes may explain the severe clinical disease and fatal outcome. Endemic dog rabies results in an ongoing risk to humans in many resource-limited and resource-poor countries, whereas rabies in wildlife is important in North America and Europe. In human cases in North America, transmission from bats is most common, but there is usually no history of a bat bite and there may be no history of contact with bats. Physicians may not recognize typical features of rabies in North America and Europe. Laboratory diagnostic evaluation for rabies includes rabies serology plus skin biopsy, cerebrospinal fluid, and saliva specimens for rabies virus antigen and/or RNA detection. Methods of postexposure rabies prophylaxis, including wound cleansing and administration of rabies vaccine and human rabies immune globulin, are highly effective after recognized exposure. Although there have been rare survivors of human rabies, no effective therapy is presently available. Therapeutic coma (midazolam and phenobarbital), ketamine, and antiviral therapies (known as the "Milwaukee protocol") were given to a rabies survivor, but this therapy was likely not directly responsible for the favorable outcome. New therapeutic approaches for human rabies need to be developed. A better understanding of basic mechanisms involved in rabies pathogenesis may be helpful in the development of potential new therapies for the future.

show abstract

“…Using iTRAQ quantitative proteomic method, Venugopal et al detected overexpression of functionally diverse proteins including karyopherin alpha 4 (KPNA4), calcium calmodulin-dependent kinase 2 alpha (CAMK2A), and glutamate ammonia ligase (GLUL) in postmortem rabies brains [69]. Likewise, protein profiling of mouse brains infected with rabies has shown differential expression of proteins involved in ion homeostasis and synaptic function [70].…”

Section: Proteomics Studies On Viral Infections Of the Nervous Systemmentioning

confidence: 98%

Application of “Omics” Technologies for Diagnosis and Pathogenesis of Neurological Infections

Noorbakhsh

Aminian

Power

2015

Curr Neurol Neurosci Rep

View full text Add to dashboard Cite

Infections of the human nervous system have substantial morbidity and mortality but also represent among the most challenging of all neurological diseases because of the difficulty in establishing a diagnosis and implementing effective therapies. Neurological infections lead to altered expression levels of a wide range of host- and pathogen-derived biomolecules both within and outside of the nervous system. Quantitative analyses of these biomolecular perturbations have been traditionally performed using "classical" molecular or analytical methods, which evaluate one or few genes or their products at a time. Recent technical developments together with the increasing availability of high-throughput/content methodologies have enabled a more comprehensive overview of these molecular alterations and thus provide new approaches to the diagnosis and/or treatment of this group of disorders. Herein, we will review recent evidence pointing to the capacity of the so-called omics techniques in studying the nervous system infections with an emphasis on genomics, transcriptomics, and proteomics technologies.

show abstract

Proteomic profiling reveals that rabies virus infection results in differential expression of host proteins involved in ion homeostasis and synaptic physiology in the central nervous system

Cited by 63 publications

References 55 publications

Mitochondrial dysfunction in rabies virus infection of neurons

Mitochondrial dysfunction in rabies virus infection of neurons

Update on rabies

Application of “Omics” Technologies for Diagnosis and Pathogenesis of Neurological Infections

Contact Info

Product

Resources

About