Proteomic profiling of the acid tolerance response (ATR) during the enhanced biomethanation process from Taihu Blue Algae with butyrate stress on anaerobic sludge

Yan, Qun; Li, Yongcan; Huang, Bin; Wang, Aijie; Zou, Hua; Miao, Hengfeng; Li, Rongqing

doi:10.1016/j.jhazmat.2012.07.062

Cited by 18 publications

(10 citation statements)

References 18 publications

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“…Metaproteomic analyses of ADs [] have already provided important insight into the different stages of anaerobic digestion (Fig. ).…”

Section: Environmental Communitiesmentioning

confidence: 99%

“…The majority of these proteins belonged to various methanogenic archaea and included the hallmark enzyme of methanogenesis, Methyl‐coenzyme M (CoM) reductase, which catalyzes the terminal step of methanogenesis , and acetyl‐CoA decarbonylase/synthase complex (ACDS), an enzyme that allows is essential for acetoclastic methanogenesis. CoM is a biomarker of methanogenesis and has been identified in all metaproteomics studies of anaerobic digesters to date and is always among the most abundant proteins []. Besides enzymes involved in methanogenesis, glycolytic enzymes from various bacteria, indicative proteins of acidogenesis have been identified.…”

Section: Environmental Communitiesmentioning

confidence: 99%

“…Basic metaproteomic protocols are established and advances in protein extraction , MS and increased availability of reference genomes have dramatically improved identification of proteins in AD. While initial studies struggled to identify around hundred proteins the identification of hundreds to thousand proteins is now possible on a routine basis .…”

Section: Environmental Communitiesmentioning

confidence: 99%

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Enhancing metaproteomics—The value of models and defined environmental microbial systems

et al. 2016

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Metaproteomics--the large-scale characterization of the entire protein complement of environmental microbiota at a given point in time--has provided new features to study complex microbial communities in order to unravel these "black boxes." New technical challenges arose that were not an issue for classical proteome analytics before that could be tackled by the application of different model systems. Here, we review different current and future model systems for metaproteome analysis. Following a short introduction to microbial communities and metaproteomics, we introduce model systems for clinical and biotechnological research questions including acid mine drainage, anaerobic digesters, and activated sludge. Model systems are useful to evaluate the challenges encountered within (but not limited to) metaproteomics, including species complexity and coverage, biomass availability, or reliable protein extraction. The implementation of model systems can be considered as a step forward to better understand microbial community responses and ecological functions of single member organisms. In the future, improvements are necessary to fully explore complex environmental systems by metaproteomics.

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“…Metaproteomic analyses of ADs [] have already provided important insight into the different stages of anaerobic digestion (Fig. ).…”

Section: Environmental Communitiesmentioning

confidence: 99%

Section: Environmental Communitiesmentioning

confidence: 99%

Section: Environmental Communitiesmentioning

confidence: 99%

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Enhancing metaproteomics—The value of models and defined environmental microbial systems

et al. 2016

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“…So far, only a few metaproteome studies of BGPs were carried out (Table 2 ) (Abram et al ., 2009; 2011 ; Hanreich et al ., 2012; 2013 ; Yan et al ., 2012 ; Heyer et al ., 2013 ; Kohrs et al ., 2014 ; Lü et al ., 2014 ). Of those, only the work of Hanreich and colleagues ( 2012 ), Heyer and colleagues ( 2013 ) and Kohrs and colleagues ( 2014 ) analysed full-scale BGPs.…”

Section: Advances In the Field Enabled By Metaproteomicsmentioning

confidence: 99%

Metaproteomics of complex microbial communities in biogas plants

Heyer

Kohrs

Reichl

et al. 2015

Microbial Biotechnology

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Production of biogas from agricultural biomass or organic wastes is an important source of renewable energy. Although thousands of biogas plants (BGPs) are operating in Germany, there is still a significant potential to improve yields, e.g. from fibrous substrates. In addition, process stability should be optimized. Besides evaluating technical measures, improving our understanding of microbial communities involved into the biogas process is considered as key issue to achieve both goals. Microscopic and genetic approaches to analyse community composition provide valuable experimental data, but fail to detect presence of enzymes and overall metabolic activity of microbial communities. Therefore, metaproteomics can significantly contribute to elucidate critical steps in the conversion of biomass to methane as it delivers combined functional and phylogenetic data. Although metaproteomics analyses are challenged by sample impurities, sample complexity and redundant protein identification, and are still limited by the availability of genome sequences, recent studies have shown promising results. In the following, the workflow and potential pitfalls for metaproteomics of samples from full-scale BGP are discussed. In addition, the value of metaproteomics to contribute to the further advancement of microbial ecology is evaluated. Finally, synergistic effects expected when metaproteomics is combined with advanced imaging techniques, metagenomics, metatranscriptomics and metabolomics are addressed.

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“…Acid stress on sludge also improves hydrogen energy recovery up to 1.4 kJ/g-TVS [6]. Under butyrate stress on sludge, Taihu blue algae exhibit 30% higher methane production rate than other algae (methane yield not reported) [7]. However, energy conversion efficiency remains slow in one step process (dark-fermentation or methanogenesis).…”

Section: Introductionmentioning

confidence: 98%

Cogeneration of hydrogen and methane from the pretreated biomass of algae bloom in Taihu Lake

Cheng

Liu

Lin

et al. 2014

International Journal of Hydrogen Energy

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Proteomic profiling of the acid tolerance response (ATR) during the enhanced biomethanation process from Taihu Blue Algae with butyrate stress on anaerobic sludge

Cited by 18 publications

References 18 publications

Enhancing metaproteomics—The value of models and defined environmental microbial systems

Enhancing metaproteomics—The value of models and defined environmental microbial systems

Metaproteomics of complex microbial communities in biogas plants

Cogeneration of hydrogen and methane from the pretreated biomass of algae bloom in Taihu Lake

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