Proteomic Profiling of Sweat Exosome Suggests its Involvement in Skin Immunity

Wu, Chi Chang; Liu, Zheng-Fei

doi:10.1016/j.jid.2017.05.040

Cited by 94 publications

(78 citation statements)

References 60 publications

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“…The sample was cooled down and centrifugated at 14 000 g for 15 minutes. After removing the supernatant, the filter‐aided sample preparation method was conducted . Briefly, dithiothreitol (Sigma, St. Louis, MO) was added to 30 μl of the sample at a final concentration of 100 mM.…”

Section: Methodsmentioning

confidence: 99%

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Jiao

Jin

Jiang

et al. 2018

J of Cellular Biochemistry

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Pancreatic cancer (PC) is one of the most lethal cancers known worldwide, and its prognosis is poor in most patients. Exosomes are nanosized extracellular vesicles, which are released from various cell types. They are involved in cellular communication. The diagnosis and treatment of PC were improved substantially with exosomes. In this study, we isolated PC-derived exosomes and investigated their proteomic profile. Then, we conducted bioinformatic analysis on proteomic data. Differential ultracentrifugation was performed to isolate exosomes from human serum samples and four PC cell lines. Transmission electron microscopy and Western blot analysis were used to characterize the isolated exosomes. Liquid chromatography coupled with tandem mass spectrometry was conducted to identify the proteome of serum exosomes. Proteomic analysis demonstrated that all the serum exosomes were derived from three cohorts of human subjects; these serum exosomes contained a total of 655 proteins, out of which 315 proteins overlapped with ExoCarta database. Gene oncology and kyoto encyclopedia of genes and genomes analyses provided the functional annotation of the proteome. Interestingly, 18 or 14 proteins were upregulated and 11 or 14 proteins were downregulated in serum exosomes derived from patients with PC as compared with in serum exosomes derived from healthy volunteers or from pancreatitis patients respectively. Annexin A11, a calcium-dependent phospholipid-binding protein, was expressed in a PC cell line (CFPAC-1)-derived exosomes and in tumor tissues of patients with PC, respectively. Our data provided a basic foundation for further studies on the protein composition of PC-derived exosomes and its involvement in PC biology.

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Section: Methodsmentioning

confidence: 99%

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Jiao

Jin

Jiang

et al. 2018

J of Cellular Biochemistry

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“…miRNAs are one of them and play an important role in intercellular cellular transport and signal transduction [73][74][75]. Exosomes can transfer metabolites and promote communication between different cells through the exchange of exosomal miRNAs, and then play an immune response, tumor microenvironment remodeling and tumor metastasis during tumor progression [38,[76][77][78]. Many reports indicate that exosomes affect the biology of recipient cells by transferring miRNAs from donor cells to recipient cells, but the mechanism of how exosomes sorting miR-NAs has not been thoroughly solved.…”

Section: Mirnas Sorting To Exosomesmentioning

confidence: 99%

Exosomal miRNAs in tumor microenvironment

Tan

Xia

et al. 2020

J Exp Clin Cancer Res

125

117

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Tumor microenvironment (TME) is the internal environment in which tumor cells survive, consisting of tumor cells, fibroblasts, endothelial cells, and immune cells, as well as non-cellular components, such as exosomes and cytokines. Exosomes are tiny extracellular vesicles (40-160nm) containing active substances, such as proteins, lipids and nucleic acids. Exosomes carry biologically active miRNAs to shuttle between tumor cells and TME, thereby affecting tumor development. Tumor-derived exosomal miRNAs induce matrix reprogramming in TME, creating a microenvironment that is conducive to tumor growth, metastasis, immune escape and chemotherapy resistance. In this review, we updated the role of exosomal miRNAs in the process of TME reshaping.

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“…Intriguingly, EVs and their biomolecule content have been found to be released at the surface of the skin and are therefore, detectable in sweat in addition to blood and other body fluids (Wu and Liu, 2018). The study of Wu and Liu (2018) was the first and thus far only study to examine sweat EVs from healthy humans after aerobic exercise (Wu and Liu, 2018). The authors performed proteomic profiling of sweat exosomes and found sweat proteins to be enriched in EVs.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise

et al. 2020

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Background: To date, microRNAs (miRs) carried in extracellular vesicles (EVs) in response to exercise have been studied in blood but not in non-invasively collectable body fluids. In the present study, we examined whether six exercise-responsive miRs, miRs-21,-26,-126,-146,-221, and-222, respond to acute endurance exercise stimuli of different intensities in sweat. Methods: We investigated the response of miRs isolated from sweat and serum EVs to three endurance exercise protocols: (1) maximal aerobic capacity (VO 2max), (2) anaerobic threshold (AnaT), and (3) aerobic threshold (AerT) tests. Sauna bathing was used as a control test to induce sweating through increased body temperature in the absence of exercise. All protocols were performed by the same subjects (n = 8, three males and five females). The occurrence of different miR carriers in sweat and serum was investigated via EV markers (CD9, CD63, and TSG101), an miR-carrier protein (AGO2), and an HDL-particle marker (APOA1) with Western blot. Correlations between miRs in sweat and serum (post-sample) were examined. Results: Of the studied miR carrier markers, sweat EV fractions expressed CD63 and, very weakly, APOA1, while the serum EV fraction expressed all the studied markers. In sweat EVs, miR-21 level increased after AerT and miR-26 after all the endurance exercise tests compared with the Sauna (p < 0.050). miR-146 after AnaT correlated to sweat and serum EV samples (r = 0.881, p = 0.004). Conclusion: Our preliminary study is the first to show that, in addition to serum, sweat EVs carry miRs. Interestingly, we observed that miRs-21 and-26 in sweat EVs respond to endurance exercise of different intensities. Our data further confirmed that miR responses to endurance exercise in sweat and serum were triggered by exercise and not by increased body temperature. Our results highlight that sweat possesses a unique miR carrier content that should be taken into account when planning analyses from sweat as a substitute for serum.

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Proteomic Profiling of Sweat Exosome Suggests its Involvement in Skin Immunity

Cited by 94 publications

References 60 publications

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Characterization and proteomic profiling of pancreatic cancer‐derived serum exosomes

Exosomal miRNAs in tumor microenvironment

MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise

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