Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response

Abstract

BackgroundChikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus.ResultsProteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis.ConclusionsThis is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.

Global protein profiling studies of chikungunya virus infection identify different proteins but common biological processes

“…Both studies utilized non‐CHIKF fever patients as controls, which implies that purely fever associated changes will not be detected in the respective analysis. Only one protein, lactotransferrin, was found in common to be differentially regulated by both studies .…”

“… Studies are identified by the first name of the author and where required, the year (Thio ; Abere ; Dhanwani (2011) ; Dhanwani (2014) ; ; Fraisier ; Wikan ; Puttamallesh ) and the protein ID or accession number listed in the study is given. Where required the proteome (liver or brain) or technique (DIGE or iTRAQ) is also indicated.…”

“…Two studies used human specimens, and Puttamallesh and colleagues identified some 63 differentially expressed proteins using iTRAQ, of which 35 proteins were more abundant whereas 28 were less abundant in CHIKF serum samples as compared with controls, whereas Wikan and colleagues investigated white blood cells and identified some 308 differentially expressed proteins of which 68 were downregulated and 240 upregulated in CHIKF samples as compared with controls. Both studies utilized non‐CHIKF fever patients as controls, which implies that purely fever associated changes will not be detected in the respective analysis.…”

“…Combined, the seven studies reported nine proteomic analyses and documented collectively more than 700 proteins differentially regulated by CHIKV infection . Combining all of the unambiguous protein information from all of the studies, only 26 proteins (Table ) were detected in more than one analysis.…”

Temporal SILAC‐based quantitative proteomics identifies host factors involved in chikungunya virus replication

“…Another study identified 3505 proteins of which only 514 were present in all samples and none of these were differentially expressed . An iTRAQ approach resulted in the identification of 569 proteins of which 63 were differentially expressed . A combination of 2D‐DIGE and iTRAQ resulted in the identification of 177 differentially expressed proteins out of a total of 2686 identified proteins .…”