Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry

DeBoer, Jason; Wojtkiewicz, Melinda; Haverland, Nicole A.; Li, Yan; Harwood, Emma; Leshen, Emily; George, Jaiben; Ciborowski, Paweł; Belshan, Michael

doi:10.1016/j.virol.2018.01.025

“…Virus replication/production was quantified by a reverse transcriptase (RT) assay as described previously (DeBoer et al ). Triplicate 10 μL of supernatant were assayed per timepoint.…”

Section: Methodsmentioning

confidence: 99%

CD4⁺T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects

Holbrook

¹

,

Peterson

²

,

Bianchi

³

et al. 2019

Self Cite

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Early studies in exercise immunology suggested acute bouts of exercise had an immunosuppressive effect in human subjects. However, recent data, show acute bouts of combined aerobic and resistance training increase both lymphocyte activation and proliferation. We quantified resistance exercise‐induced changes in the activation state of CD4+ T lymphocytes via surface protein expression and using a medically relevant model of infection (HIV‐1). Using a randomized cross‐over design, 10 untrained subjects completed a control and exercise session. The control session consisted of 30‐min seated rest while the exercise session entailed 3 sets × 10 repetitions of back squat, leg press, and leg extensions at 70% 1‐RM with 2‐min rest between each set. Venous blood samples were obtained pre/post each session. CD4+ T lymphocytes were isolated from whole blood by negative selection. Expression of activation markers (CD69 & CD25) in both nonstimulated and stimulated (costimulation through CD3+CD28) cells were assessed by flow cytometry. Resistance exercised‐induced effects on intracellular activation was further evaluated via in vitro infection with HIV‐1. Nonstimulated CD4+ T lymphocytes obtained postexercise exhibited elevated CD25 expression following 24 h in culture. Enhanced HIV‐1 replication was observed in cells obtained postexercise. Our results demonstrate that an acute bout of resistance exercise increases the activation state of CD4+ T lymphocytes and results in a greater susceptibility to HIV‐1 infection in vitro. These findings offer further evidence that exercise induces activation of T lymphocytes and provides a foundation for the use of medically relevant pathogens as indirect measures of intracellular activation.

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“…Vimentin has been found to interact with hepatitis C virus (HCV) core protein, which regulates its expression, impacting virus replication [157]. Vimentin is also important for efficient infection by HIV [158,159], through mechanisms that are not yet well understood. Interestingly, DeBoer et al [158] also found that abundance of vimentin is increased in the cytoplasmic and membrane fractions in infected cell cultures.…”

Section: Viral Infectionsmentioning

confidence: 99%

“…Vimentin is also important for efficient infection by HIV [158,159], through mechanisms that are not yet well understood. Interestingly, DeBoer et al [158] also found that abundance of vimentin is increased in the cytoplasmic and membrane fractions in infected cell cultures. Conversely, vimentin expression has been observed to be negatively affected by virus infection in some cases.…”

Section: Viral Infectionsmentioning

confidence: 99%

Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections

Ramos

¹

,

Stamatakis

²

,

Oeste

³

et al. 2020

IJMS

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Vimentin is an intermediate filament protein that plays key roles in integration of cytoskeletal functions, and therefore in basic cellular processes such as cell division and migration. Consequently, vimentin has complex implications in pathophysiology. Vimentin is required for a proper immune response, but it can also act as an autoantigen in autoimmune diseases or as a damage signal. Although vimentin is a predominantly cytoplasmic protein, it can also appear at extracellular locations, either in a secreted form or at the surface of numerous cell types, often in relation to cell activation, inflammation, injury or senescence. Cell surface targeting of vimentin appears to associate with the occurrence of certain posttranslational modifications, such as phosphorylation and/or oxidative damage. At the cell surface, vimentin can act as a receptor for bacterial and viral pathogens. Indeed, vimentin has been shown to play important roles in virus attachment and entry of severe acute respiratory syndrome-related coronavirus (SARS-CoV), dengue and encephalitis viruses, among others. Moreover, the presence of vimentin in specific virus-targeted cells and its induction by proinflammatory cytokines and tissue damage contribute to its implication in viral infection. Here, we recapitulate some of the pathophysiological implications of vimentin, including the involvement of cell surface vimentin in interaction with pathogens, with a special focus on its role as a cellular receptor or co-receptor for viruses. In addition, we provide a perspective on approaches to target vimentin, including antibodies or chemical agents that could modulate these interactions to potentially interfere with viral pathogenesis, which could be useful when multi-target antiviral strategies are needed.

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“…Vimentin has been found to interact with hepatitis C virus (HCV) core protein, which regulates its expression, impacting virus replication [136]. Vimentin is also important for efficient infection by HIV [137,138], through mechanisms that are not yet well understood. Interestingly, DeBoer et al (2018) also found that abundance of vimentin is increased in the cytoplasmic and membrane fractions in infected cell cultures.…”

Section: Viral Infectionsmentioning

confidence: 99%

Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections

Ramos

¹

,

Stamatakis

²

2020

Preprint

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Vimentin is an intermediate filament protein that plays key roles in integration of cytoskeletal functions, and therefore in basic cellular processes such as cell division and migration. Consequently, vimentin has complex implications in pathophysiology. Vimentin is required for a proper immune response, but it can also act as an autoantigen in autoimmune diseases or as a damage signal. Although vimentin is a predominantly cytoplasmic protein, it can also appear at extracellular locations, either in a secreted form or at the surface of numerous cell types, often in relation to cell activation, inflammation, injury or senescence. Cell surface targeting of vimentin appears to associate with the occurrence of certain posttranslational modifications, such as phosphorylation and/or oxidative damage. At the cell surface, vimentin can act as a receptor for bacterial and viral pathogens. Indeed, vimentin has been shown to play important roles in virus attachment and entry of severe acute respiratory syndrome-related coronavirus (SARS-CoV), dengue and encephalitis viruses, among others. Moreover, the presence of vimentin in specific virus-targeted cells and its induction by proinflammatory cytokines and tissue damage contribute to its implication in viral infection. Here, we recapitulate some of the pathophysiological implications of vimentin, including the involvement of cell surface vimentin in interaction with pathogens, with a special focus on its role as a cellular receptor or co-receptor for viruses. In addition, we provide a perspective on approaches to target vimentin, including antibodies or chemical agents that could modulate these interactions to potentially interfere with viral pathogenesis, which could be useful when multi-target antiviral strategies are needed .

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Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry

Cited by 7 publications

References 60 publications

CD4⁺T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects

CD4⁺T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects

Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections

Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections

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Proteomic profiling of HIV-infected T-cells by SWATH mass spectrometry

Cited by 7 publications

References 60 publications

CD4+T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects

CD4+T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects

Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections

Vimentin as a Multifaceted Player and Potential Therapeutic Target in Viral Infections

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Product

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CD4⁺T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects

CD4⁺T cell activation and associated susceptibility to HIV‐1 infection in vitro increased following acute resistance exercise in human subjects