Proteomic profiling of fast-to-slow muscle transitions during aging

Ohlendieck, Kay

doi:10.3389/fphys.2011.00105

Cited by 45 publications

(70 citation statements)

References 37 publications

(47 reference statements)

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“…Impairments in mobility, falls and fear of falling (30,31) are therefore of prognostic value (32). Impaired mobility in the elderly, frequent falls and poor balance are connected with the increase in sarcopenia (33,34). In our set we found a correlation between age and mobility problems (Tab.…”

Section: Discussionsupporting

confidence: 55%

Geriatric giants – contemporary occurrence in 12,210 in-patients

Weber¹,

Meluzínová²,

MatějovskáKubešová³

et al. 2015

BLL

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OBJECTIVES: With advancing age, the degree of dependency and occurrence of great geriatric syndromes (GS), also referred to as geriatric giants, grow substantially. DESIGN: The prospective cohort study was aimed at conducting an analysis and comparison of geriatric syndromes (geriatric giants) among different age groups at admission to the acute geriatric department. SETTING, PARTICIPANTS: Between 1995 and 2012, we had altogether 12,210 elderly patients at an average age of 80.5 ± 7.0 y (range 65-103 y) hospitalised at the Department of Geriatrics. We divided the patient set into three different age subgroups (65-74 y; 75-84 y and ≥ 85 y; e.g. 21.4 %; 47.9 % and 30.7 %) and compared the results among them. RESULTS: 3,787 persons (31.0 %) were without any GS. The growing tendency of the occurrence of all geriatric syndromes in combinations with increased age (p < 0.001) is obvious. Their occurrence in the above mentioned different age sets was examined in relation to individual geriatric syndromes and sex (female and male), namely falls 22.0 %, 27.8 %, 39.9 % and 20.5 %, 27.0 %, 36.1 %; immobility 26.4 %, 29.3 %, 42.5 % and 30.3 %, 30.1 % and 39.2 %; incontinence 38.4 %, 50.6 %, 69.5 % and 38.2 %, 47.4 %, 61.8 %; dementia and cognitive impairment 13.4 %, 23.4 %, 38.1 % and 15.8 %, 24.3 %, 33.2 % respectively. Age cut-off for geriatric syndromes occurrence based on ROC analysis is 83.5-84.5 y for females and 78.5-82.5 y for males. CONCLUSION: The occurrence of geriatric giants increasing with age and female gender is of crucial importance not only for individuals and families but also for demands on costs of health and social care in oncoming decades (Tab. 6, Fig. 3, Ref. 52). Text in PDF www.elis.sk.

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Section: Discussionsupporting

confidence: 55%

Geriatric giants – contemporary occurrence in 12,210 in-patients

Weber¹,

Meluzínová²,

MatějovskáKubešová³

et al. 2015

BLL

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“…However, aging is characterized by a switch from fast- to slow-twitch muscle fiber types (Ohlendieck, 2011), and leucine has been shown to have a greater effect on CSA of Type I fibers (Baptista et al, 2010). Therefore, differential effect of leucine supplementation in aged mice compared to young mice may be due to aged-induced alterations in fiber type.…”

Section: Discussionmentioning

confidence: 99%

Differential effects of leucine supplementation in young and aged mice at the onset of skeletal muscle regeneration

Perry

Brown

Lee

et al. 2016

Mechanisms of Ageing and Development

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Aging decreases the ability of skeletal muscle to respond to injury. Leucine has been demonstrated to target protein synthetic pathways in skeletal muscle thereby enhancing this response. However, the effect of aging on leucine-induced alterations in protein synthesis at the onset of skeletal muscle regeneration has not been fully elucidated. The purpose of this study was to determine if aging alters skeletal muscle regeneration and leucine-induced alterations in markers of protein synthesis. The tibialis anterior of young (3 months) and aged (24 months) female C57BL/6J mice were injected with either bupivacaine or PBS, and the mice were given ad libitum access to leucine-supplemented or normal drinking water. Protein and gene expression of markers of protein synthesis and degradation, respectively, were analyzed at three days post-injection. Following injury in young mice, leucine supplementation was observed to elevate only p-p70S6K. In aged mice, leucine was shown to elicit higher p-mTOR content with and without injury, and p-4EBP-1 content post-injury. Additionally in aged mice, leucine was shown to elicit higher content of relative p70S6K post-injury. Our study shows that leucine supplementation affects markers of protein synthesis at the onset of skeletal muscle regeneration differentially in young and aged mice.

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“…The pathophysiological mechanisms of sarcopenia are believed to be highly complex resulting in a multi-factorial etiology. Recent proteomic studies have established extensive changes in the skeletal muscle proteome during the natural aging process [210,211,212], whereby gel-based proteomics has been instrumental in the initial cataloguing of the aged muscle proteome [213,214,215]. Skeletal muscle aging is closely associated with persistent impairments of the peripheral nervous system, which trigger excitation-contraction uncoupling and pathophysiological cycles of denervation and faulty reinnervation.…”

Section: Comparative Skeletal Muscle Proteomics Using Two-dimensiomentioning

confidence: 99%

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

Murphy¹,

Dowling²,

Ohlendieck³

2016

Proteomes

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The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021). The application of two-dimensional gel electrophoresis has played a key role in the systematic identification and detailed characterization of the protein constituents of skeletal muscles. Protein changes during myogenesis, muscle maturation, fibre type specification, physiological muscle adaptations and natural muscle aging were studied in depth by the original O’Farrell method or slightly modified gel electrophoretic techniques. Over the last 40 years, the combined usage of isoelectric focusing in the first dimension and sodium dodecyl sulfate polyacrylamide slab gel electrophoresis in the second dimension has been successfully employed in several hundred published studies on gel-based skeletal muscle biochemistry. This review focuses on normal and physiologically challenged skeletal muscle tissues and outlines key findings from mass spectrometry-based muscle proteomics, which was instrumental in the identification of several thousand individual protein isoforms following gel electrophoretic separation. These muscle-associated protein species belong to the diverse group of regulatory and contractile proteins of the acto-myosin apparatus that forms the sarcomere, cytoskeletal proteins, metabolic enzymes and transporters, signaling proteins, ion-handling proteins, molecular chaperones and extracellular matrix proteins.

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Proteomic profiling of fast-to-slow muscle transitions during aging

Cited by 45 publications

References 37 publications

Geriatric giants – contemporary occurrence in 12,210 in-patients

Geriatric giants – contemporary occurrence in 12,210 in-patients

Differential effects of leucine supplementation in young and aged mice at the onset of skeletal muscle regeneration

Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis

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