Proteomic profiling of cerebrospinal fluid in parkinsonian disorders

Blood and cerebrospinal fluid markers in Parkinson's disease: current biomarker findings

“…A study of CSF proteins compared surface-enhanced laser desorption/ionization time-of-flight mass spectrometry in 56 subjects with PD, 24 control subjects, as well as samples from individuals with the clinically closely related disorders of multiple system atrophy, progressive supranuclear palsy, and corticobasal degeneration. CSF levels of ubiquitin, beta2-microglobulin, and two secretogranin 1 fragments differentiated PD from atypical parkinsonism, 83 although they did not differentiate PD from controls. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), other protein species have been identified that are differentially expressed between PD and controls, [84][85][86] and recent analysis of proteins captured by magnetic bead-based weak cation exchange followed by MALDI-TOF-MS in a blinded analysis identified a combination of five biomarkers discriminating PD and healthy controls with 85% sensitivity and 70% specificity.…”

Cerebrospinal fluid biomarkers in parkinsonian conditions: an update and future directions

“…Using surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI-TOF MS), Constantinescu et al 94 found a CSF proteomic profile consisting of four proteins (ubiquitin, β2-microglobulin and two secretographin 1 fragments), which differentiated PD and healthy controls from atypical parkinsonian patients with an AUC of 0.8. Recently, Ishigami et al 95 were able to differentiate PD from MSA, even at the early stages, using their proteomic pattern (ie, the combined set of many protein peaks), rather than a single peak.…”

The Potential of Proteomics in Understanding Neurodegeneration

“…Other neurodegenerative diseases such as atypical parkinsonian disorders (APD), including multiple system atrophy and progressive supranuclear palsy, have also been studied by SELDI-TOF-MS. Proteins such as ubiquitin, β2-microglobulin, and two secretogranin 1 fragments were identified that differentiated healthy individuals from individuals with APD (Constantinescu et al, 2010).…”