Proteomic profiling for ovarian development and azadirachtin exposure in Spodoptera litura during metamorphosis from pupae to adults

Sun, Ranran; Xu, Yuanhao; Jin, Liu; Yang, Lan; Cui, Gaofeng; Zhong, Guohua; Yi, Xin

doi:10.1016/j.ecoenv.2022.113548

Cited by 6 publications

(5 citation statements)

References 53 publications

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“…For insects, pupation and emergence are key scenarios of metamorphosis [ 33 , 34 ]. Knocking down GpylFAS1 or GpylDesat5 , which are essential for metamorphosis in G. pyloalis , leads to more abnormal pupae and adults, and lower emergence rates [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Song

Liu

et al. 2022

IJMS

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Fatty acid synthase (FAS) is a key enzyme in the lipid synthesis pathway, however, its roles in insects remain largely unknown. Here, we firstly identified two FAS genes from the transcriptome dataset of the general cutworm Spodoptera litura, which is a destructive insect pest of many crops. Both SlFAS1 and SlFAS2 were highly expressed in third instar larvae and in their fat bodies. Then, we successfully silenced SlFAS1 in third instar larvae and the content of α-linolenic acid and triglyceride was significantly decreased. Besides that, the effect of FAS on the metamorphic development in S. litura was evaluated. The results indicate that after silencing SlFAS1, the survival rates of S. litura larvae decreased significantly compared to the control groups. Silencing SlFAS1 in fifth instar larvae resulted in more malformed pupae and adults, and the emergence rates were significantly reduced. Furthermore, the ecdysone content in the haemolymph of fifth instar larvae was significantly decreased after silencing SlFAS1. In addition, knocking down SlFAS1 significantly alters the expression of other key genes in the lipogenesis pathway, implying that FAS has an impact on the lipogenesis pathway. The present study deepens the understanding of FAS in insects and provides novel potential targets for managing insect pests.

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Section: Discussionmentioning

confidence: 99%

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Song

Liu

et al. 2022

IJMS

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“…CF females showed inverse allometric growth (allometric coefficient < 0) for ovary growth. Prior research indicates that larvae reared on the CF diet have a significantly low protein content (Zhang et al 2023b), a vital component for insect ovarian development (Wei et al 2017, Sun et al 2022). This protein deficiency in CF females may constrain the proportional growth of ovary weight with body weight for females, leading to the inverse allometry observed in our study.…”

Section: Discussionmentioning

confidence: 99%

Does What You Eat Affect How You Mate? Disentangling the Interactions Between Diet-Induced Phenotypic Plasticity and Adult Reproductive Strategies in Black Soldier Flies

Zhang,

Ng,

Chin

et al. 2024

Preprint

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Phenotypic plasticity enables organisms to response to environmental variations by generating a range of phenotypes from a single genotype. In holometabolous insects, traits that influence larval plasticity may hold relevance for adult life history strategies. We present a comprehensive investigation into phenotypic plasticity in black soldier flies, a species known for its efficient waste-to-biomass conversion in the larval stage. Here, we document adult sex-specific plastic responses and reproductive strategies shaped by larval diets. We examined traits including adult body size, reproductive organ development, sperm length, mating behaviours, egg production and other life history parameters across different treatments. Our findings reveal notable sex-specific differences in phenotypic plasticity, with females showing increased plasticity in reproductive investment. Furthermore, males and females differed starkly in allometric growth and weight ratio of reproductive organs. Diets that facilitated longer male lifespans also prompted earlier male emergence suggesting an interplay between lifespan and degree of protandry. This maximizes the overlap of male and female lifespans, thereby enhancing mating success in diverse environmental conditions. Our results reveal plastic responses in mating behaviours, where diets producing smaller adults, smaller reproductive organs, and shorter sperm correlated with significantly enhanced mating effort and performance. This study highlights the complex interactions between nutrition, development, and reproductive strategies, and has significant implications for the insect bioconversion industries.

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“…To detect the stage-specific expression patterns of SlRps , we collected the ovarian fragments of previtellogenesis stage (PS), vitellogenesis stage (VS.) and choriogenesis stage (CS) from female adults (2rd day after eclosion) based on the presence and positions of nurse cells ( Sun et al, 2022 ). The total RNA was extracted by RNAiso Plus (TaKaRa, Japan), and the first-strand cDNA was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the manufacturer’s instructions (Takara Biomedical Technology, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…Thus, S. litura has been extensively studied as a representative pest in agricultural ecosystem. The ovary of S. litura belongs to polytrophic meroistic ovarian, and the oogenesis can be sequentially subdivided into three developmental periods: previtellogenesis, vitellogenesis and choriogenesis ( Sun et al, 2022 ), which may require many genes for the synthesis of those functional proteins. The released genome of S. litura could provide important resources for further molecular researches of this species (assembly ASM270686v3) ( Cheng et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Stage-Specific Expression Profile Analysis Reveal the Function of Ribosomal Proteins for Oogenesis of Spodoptera litura

Sun

Liu

et al. 2022

Front. Physiol.

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Ribosomal proteins (Rps) are indispensable in ribosome biogenesis and protein synthesis, which tightly correlate with cell growth and proliferation in different physiological processes across species. Up to now, genes coding for Rps have been identified and studied in many species, however, their information still remains elusive in many insect species, especially in Spodoptera litura. In this study, 81 Rp genes were identified from S. litura genome and were mapped to their positions on the chromosomes. In addition, their physical and chemical properties, gene structure, phylogenetic relationships, targeted microRNAs were also analyzed. Gene ontology analysis disclosed that Rp genes were closely associated with processes related to ribosome biosynthesis, proteins translation processing, molecular binding activities. The quantitative real-time PCR (qRT-PCR) revealed expression profiles of Rp genes varied in different stages of oogenesis, and found that most Rp genes accumulated in previtellogenesis stage. This study described the comprehensive genome-wide analysis of Rp gene family in agricultural pests, which provided foundation for further characterizing the roles of Rps in oogenesis of insects, and some Rp genes may further serve as targets for innovative pest control.

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Proteomic profiling for ovarian development and azadirachtin exposure in Spodoptera litura during metamorphosis from pupae to adults

Cited by 6 publications

References 53 publications

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

The Key Role of Fatty Acid Synthase in Lipid Metabolism and Metamorphic Development in a Destructive Insect Pest, Spodoptera litura (Lepidoptera: Noctuidae)

Does What You Eat Affect How You Mate? Disentangling the Interactions Between Diet-Induced Phenotypic Plasticity and Adult Reproductive Strategies in Black Soldier Flies

Genome-Wide Identification and Stage-Specific Expression Profile Analysis Reveal the Function of Ribosomal Proteins for Oogenesis of Spodoptera litura

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