Proteomic profile of the acquired enamel pellicle of professional wine tasters with erosive tooth wear

Silva, Natália Caldeira; Ventura, Talita Mendes Oliveira; Oliveira, Bethania Paludo de; Santos, Nicole Marchioro dos; Pelá, Vinícius Taioqui; Buzalaf, Marília Afonso Rabelo; Rodrigues, Jonas Almeida

doi:10.1111/eos.12779

Cited by 5 publications

(2 citation statements)

References 44 publications

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“…25 As mentioned above, several intracellular proteins delivered to saliva after exfoliation of oral mucosa cells have the potential to bind to hydroxyapatite, or participate in supramolecular aggregates that bind to the precursor proteins in the AEP. This has also been observed in other recent studies, [26][27][28][29][30][31] and could have a central role in protecting the tooth surface against acid dissolution. Hence, it is worthwhile noting that not only do secreted salivary proteins participate in AEP formation, but oral mucosa proteins, gingival crevicular fluid and even bacteria also act strongly on the enamel pellicle.…”

Section: This Process Is Based On the Epigenetic Mechanisms Associate...supporting

confidence: 84%

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

MARTINI,

RIOS,

DIONIZIO

et al. 2023

Braz. oral res.

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The objective of this study was to compare the protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without gastroesophageal reflux disease (GERD), and with or without erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD and ETW; 2) GERD without ETW; and 3) control (without GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by Protein Lynx Global Service software. A total of 213 proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and calciumbinding proteins. Twenty-three proteins were found in all the groups, including 14-3-3 protein zeta/delta and 1-phosphatidylinositol. Several intracellular proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor proteins in the AEP. Proteins might play a central role in protecting the dental surface against acid dissolution.

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Section: This Process Is Based On the Epigenetic Mechanisms Associate...supporting

confidence: 84%

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

MARTINI,

RIOS,

DIONIZIO

et al. 2023

Braz. oral res.

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“…The amount of proteins commonly found for both groups draws attention. Previous in vivo studies and an in situ study [8, 48, 49] identified 76, 57, and 68 proteins, respectively, in common when comparing the acquired enamel pellicle of groups of adults. In our study, only 37 proteins were identified as common for both groups, which is much less than published previously.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Oliveira

Buzalaf

Silva

et al. 2023

European J Oral Sciences

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Acquired enamel pellicle plays an important role in the pathogenesis of early childhood caries (ECC), working as a protective interface between the tooth and the oral cavity. The aim of this cross-sectional in vivo proteomic study was to compare the acquired enamel pellicle protein profile of 3-5-year-old children with ECC (n = 10) and caries-free children (n = 10). Acquired enamel pellicle samples were collected and processed for proteomic analysis (nLC-ESI-MS/MS). In total, 241 proteins were identified. Basic salivary proline-rich protein 1 and 2, Cystatin-B, and SA were found only in the caries free group. When comparing caries free and ECC groups, lower protein levels were found in the caries free group for hemoglobin subunit beta, delta, epsilon, gamma-2, globin domain-containing protein and gamma-1, neutrophil defensin 3, serum albumin, protein S100-A8, and S100-A9. The proteins histatin-1, statherin, salivary acidic proline-rich phosphoprotein ½, proline-rich protein 4, submaxillary gland androgen-regulated protein 3B, alpha-amylase 1 and 2B were found at higher levels in the caries free group. The exclusive and the proteins found at higher levels in the caries free group might have protective functions that play a role in the prevention of caries, besides providing important insights to be evaluated in future studies for the possible development of new therapeutic strategies for ECC.

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Relationship between erosive tooth wear and possible etiological factors among dental students

et al. 2022

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Proteomic profile of the acquired enamel pellicle of professional wine tasters with erosive tooth wear

Cited by 5 publications

References 44 publications

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

Acquired enamel pellicle protects gastroesophageal reflux disease patients against erosive tooth wear

Proteomic profile of the acquired enamel pellicle of children with early childhood caries and caries‐free children

Relationship between erosive tooth wear and possible etiological factors among dental students

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