Proteomic profile of serum of pregnant women carring a fetus with Down syndrome using nano uplc Q-tof ms/ms technology

Uriarte, Graciela Arelí López; Burciaga-Flores, Carlos Horacio; Cruz, Víctor Manuel Torres de la; Aguado, María Magdalena Medina; Puente, Viviana Maricela Gómez; Gutiérrez, Liliana Nayeli Romero; Villarreal, L.

doi:10.1080/14767058.2017.1319923

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…Proteomic results are constantly changing, showing how sets of proteins interact with environmental factors [ 40 ]. Furthermore, the concentrations of many proteins depend on their locations in biological compartments and the phase of the cell cycle, which can also be interrupted by many diseases [ 41 , 42 ]. In the results described above, we see differences depending on the selected research group and control group.…”

Section: Markers In the Tears Of Patients With Graves’ Orbitopathymentioning

confidence: 99%

Tears as a Source of Biomarkers in the Diagnosis of Graves’ Orbitopathy

et al. 2022

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Thyroid eye disease (TED) is a poorly understood autoimmune manifestation of thyroid diseases most commonly associated with Graves’ disease. Due to a lack of specific biomarkers and uncertain signs and symptoms, Graves’ orbitopathy (GO) is challenging to diagnose early and treat effectively. Nowadays, there is great interest in searching for precise molecular biomarkers for early detection, disease monitoring, and clinical management. Researchers are keen to identify novel methods to predict and diagnose diseases and to monitor patient therapeutic response. Tears, due to their direct contact with the eye and the fact that lacrimal glands can also be affected by the disease, could give new insights into the mechanisms taking place in thyroid-associated orbitopathy (TAO) and reveal potential promising biomarkers. Tear fluid offers the possibility of the non-invasive acquisition of a sample with a high protein content, thereby attracting continuously growing interest in the discovery of novel biomarkers. This article provides an up-to-date overview of the various putative tear-fluid biomarkers that have been identified. In this review, we present the potential use of tears as a diagnostic fluid and tool to investigate the mechanism of ocular diseases and discuss the future research directions in this area.

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Section: Markers In the Tears Of Patients With Graves’ Orbitopathymentioning

confidence: 99%

Tears as a Source of Biomarkers in the Diagnosis of Graves’ Orbitopathy

et al. 2022

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“…Proteomics could also enable the detection of novel and more affordable T21 biomarkers [ 68 , 69 ]. Accordingly, Charkiewicz et al suggested that imbalance in the level of circulating proteins in maternal blood can stimulate an immune response producing autoantibodies.…”

Section: Proteomics and Down Syndrome Screeningmentioning

confidence: 99%

“…At the same time, proteomic results showing how sets of proteins interact with environmental factors are constantly changing [ 85 ]. Furthermore, the concentrations of many proteins depend on their locations in biological compartments and the phase of the cell cycle, which can also be interrupted by many diseases [ 68 , 69 ]. However, the extensive software required for utilizing proteomic data and the need for highly proficient technicians substantially increase the cost.…”

Section: Proteomics and Down Syndrome Screeningmentioning

confidence: 99%

Novel Approaches to an Integrated Route for Trisomy 21 Evaluation

et al. 2021

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Trisomy 21 (T21) is one of the most commonly occurring genetic disorders, caused by the partial or complete triplication of chromosome 21. Despite the significant progress in the diagnostic tools applied for prenatal screening, commonly used methods are still imprecise and involve invasive diagnostic procedures that are related to a maternal risk of miscarriage. In this case, novel prenatal biomarkers are still being evaluated using highly specialized techniques, which could increase the diagnostic usefulness of biochemical prenatal screening for T21. From the other hand, the T21′s pathogenesis, caused by the improper division of genetic material, disrupting many metabolic pathways, could be further evaluated with the use of omics methods, which could result in bringing relevant insights for the evaluation of potential medical targets. Accordingly, a literature search was undertaken to collect novel information about prenatal screening for Down syndrome with the use of advanced technology, with a particular emphasis on the evaluation of novel screening biomarkers and the discovery of potential medical targets. These meta-analyses are focused on novel approaches designed with the use of omics techniques, representing the most rapidly developing and promising field in research today. Considering the limitations and progress of these methods, the use of omics techniques in evaluating T21 pathogenesis could bring beneficial results in prenatal screening, simultaneously uncovering novel potential medical targets.

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“…We thus surveyed the proteomics literature to ascertain whether Cpn10 (or Hsp10) was actually present in human pregnancy serum. In 6 studies [21][22][23][24][25] only one study identified one Hsp10 peptide [26], although 100-800 other proteins were identified in these studies. Hsp10 is not intrinsically difficult to detect by mass spectroscopy [27,28].…”

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confidence: 99%