Proteomic profile of pre‐implantational ovine embryos produced in vivo

Sanchez, Deisy Johana Diaz; Vasconcelos, Fábio Perdigão; Teles-Filho, Antônio C A; Viana, Arabela Guedes de Azevedo; Martins, Aline Maria Araújo; Sousa, Marcelo Valle de; Castro, Mariana S.; Ricart, Carlos A. O.; Fontes, Wagner; Bertolini, Marcelo; Bustamante‐Filho, Ivan Cunha; Moura, Arlindo A.

doi:10.1111/rda.13897

Cited by 14 publications

(20 citation statements)

References 112 publications

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“…Interestingly, oviduct-specific glycoprotein (OVGP1), also known as oviductin, was detected among the most abundant proteins at all stages. Oviductin was identified in the proteomic profile of ovine blastocyst developed in vivo ( Sanchez et al, 2021 ) and as a major embryo-interacting protein in the bovine oviduct fluid ( Banliat et al, 2020 ). Oviductin has been reported to interact with embryos in various mammalian species ( Aviles et al, 2010 ) and improves the quality of in vitro produced cattle embryos when added as a recombinant protein in culture medium ( Algarra et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes in the Proteome of Early Bovine Embryos Developed In Vivo

Banliat

Mahé

Lavigne

et al. 2022

Front. Cell Dev. Biol.

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Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and early cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed in vivo. Eleven females were used as embryo donors and pools of embryos at the 4–6 cell, 8–12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with label free quantitative mass spectrometry. A total of 2,757 proteins were identified, of which 1,950 were quantitatively analyzed. Principal component analysis of data showed a clear separation of embryo pools according to their developmental stage. The hierarchical clustering of differentially abundant proteins evidenced a first cluster of 626 proteins that increased in abundance during development and a second cluster of 400 proteins that decreased in abundance during development, with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis, whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. The pairwise comparison between stages allowed us to identify specific protein interaction networks and metabolic pathways at the time of EGA, morula compaction and blastocyst formation. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo. These data provide a number of protein candidates that will be useful for further mechanistic and functional studies.

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Section: Discussionmentioning

confidence: 99%

Dynamic Changes in the Proteome of Early Bovine Embryos Developed In Vivo

Banliat

Mahé

Lavigne

et al. 2022

Front. Cell Dev. Biol.

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“…Among the 11 DEmiRs that we identified in human frozen-thawed sperm, hsa-miR-106b-5p ( Wei et al, 2017 ; He et al, 2020 ), hsa-miR-590-3p ( Shi X. et al, 2020 ), hsa-miR-328-3p ( Bai et al, 2021 ), and hsa-miR-140-5p ( Nie et al, 2019 ; Jiao et al, 2020 ) may cause a decreased fertilization rate in IVF and ICSI cases when using cryopreserved sperm since these miRNAs are all related to cell cycle and mitosis. miR-106b-5p is a key miRNA morula and blastocyst developmental state, having potential interactions with embryo-expressed genes ( Sanchez et al, 2021 ). Upregulated miR-328-3p is a biomarker in teratozoospermia and may decrease fertilization and embryo formation rate ( Corral-Vazquez et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Zhang

et al. 2021

Front. Cell Dev. Biol.

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Although sperm preservation is a common means of personal fertility preservation, its effects on embryonic development potential need further investigation. The purpose of this study was to identify key microRNA (miRNA) in cryopreserved sperm and determine the changes of these miRNAs and their target genes during embryonic development using cryopreserved sperm. Moreover, the embryonic development potential of cryopreserved sperm was estimated in assisted reproductive technology (ART), where key miRNAs and target genes were validated in sperm and subsequent embryos. Clinical data of embryonic development from cryopreserved sperm indicated a significant decrease in fertilization rate in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cases, as well as a reduction in blastocyst formation rate in ICSI cases. Meanwhile there was a significant increase in blocked embryo ratio of Day1, Day2, and Day3.5 embryos when frozen-thawed mouse sperm was used, compared with fresh mouse sperm, suggesting a potential negative effect of sperm cryopreservation on embryonic development. From frozen-thawed and fresh sperm in humans and mice, respectively, 21 and 95 differentially expressed miRNAs (DEmiRs) were detected. miR-148b-3p were downregulated in both human and mouse frozen-thawed sperm and were also decreased in embryos after fertilization using cryopreserved sperm. Target genes of miR-148b-3p, Pten, was identified in mouse embryos using quantitative real-time PCR (qRT-PCR) and Western blot (WB). In addition, common characters of cryopreservation of mouse oocytes compared with sperm were also detected; downregulation of miR-148b-3p was also confirmed in cryopreserved oocytes. In summary, our study suggested that cryopreservation of sperm could change the expression of miRNAs, especially the miR-148b-3p across humans and mice, and may further affect fertilization and embryo development by increasing the expression of Pten. Moreover, downregulation of miR-148b-3p induced by cryopreservation was conserved in mouse gametes.

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“…Genes defined with gene ontology terms were screened for potential modulation by miRNAs, using the Homo sapiens dataset of mIRBase (http://www.mirbase.org); (Kozomara & Griffiths‐Jones, 2011), once the search mechanisms were not available for Ovis aries miRNA data. To obtain the interaction between miRNAs and genes, data were submitted to miRNet 2.0 server (https://www.mirnet.ca/) (Chang et al., 2020; Sanchez et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

“…Despite these promising results, the knowledge about the proteomic signatures of embryo development remains limited in the case of small ruminant species. Recently, our group described the first major proteomic profile of 6‐day‐old ovine embryos produced in vivo, reporting 667 proteins that were mainly related to functional clusters defined as energy metabolism (TCA cycle, pyruvate and glycolysis metabolism), zona pellucida (ZP), MAPK signalling pathway, tight junction, ZP sperm binding, translation, proteasome and cell cycle, among others (Sanchez et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

Global proteomic analysis of preimplantational ovine embryos produced in vitro

Passos

Guerreiro

Otávio

et al. 2022

Reprod Domestic Animals

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The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were extracted separately from three pools of 45 embryos and separately run in SDS‐PAGE. Proteins from each pool were individually subjected to in‐gel digestion followed by LC‐MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6‐day‐old ovine embryos, including albumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma‐actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen binding. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule‐based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxidative phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP‐PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep embryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.

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Proteomic profile of pre‐implantational ovine embryos produced in vivo

Cited by 14 publications

References 112 publications

Dynamic Changes in the Proteome of Early Bovine Embryos Developed In Vivo

Dynamic Changes in the Proteome of Early Bovine Embryos Developed In Vivo

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Global proteomic analysis of preimplantational ovine embryos produced in vitro

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