Proteomic profile of in situ acquired pellicle on tooth and restorative material surfaces

Hu, Hong-Ying; Burrow, Michael F.; Leung, Wai Keung

doi:10.1016/j.jdent.2022.104389

Cited by 2 publications

(9 citation statements)

References 59 publications

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“…A similar study found 70 different peaks on CM10 chips (39 in the range of 2-10 kDa, 17 from 10-20 kDa, and 14 from 20-100 kDa) and a total of 108 peaks on the Q10 chips with most peaks in the range of below 10 kDa [43]. These findings are in line with the results of the present investigation, while other studies reported from total numbers that ranged between 70 and >1000 proteins [14,[44][45][46].…”

Section: Discussionsupporting

confidence: 92%

“…Besides bacterial attachment functions, it was realized that the physicochemical surface properties of dental materials are also altered by the established pellicle. Detailed examinations have shown that certain surface characteristics such as wettability and surface-free energy of the substrate can strongly be changed due to the pellicle formation [3,14,20,36].…”

Section: Discussionmentioning

confidence: 99%

“…Besides carbohydrates and lipids, proteins are the main component of an acquired salivary pellicle. Overall, up to 1000 different proteins can be detected [14]. Out of these, phosphoproteins are the most common.…”

Section: Introductionmentioning

confidence: 99%

“…Out of these, phosphoproteins are the most common. Other proteins that are especially rich in glutamic acid, glycine, alanine, serine, and proline are also usually present in high numbers [5,[13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Up to now, significant differences in the ultrastructural appearance of in situ-formed pellicle layers on enamel and restorative materials such as ceramics, cement, resin-based composites, or titanium are still controversially discussed [14,18,19]. While some authors report variations in the accumulation of specific proteins such as secretory immunoglobulin A, immunoglobulin G, peroxidases, thiocyanate, and lysozyme, others did not observe any significant differences [15,[20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

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Salivary Pellicle Formed on Dental Composites Evaluated by Mass Spectrometry—An In Situ Study

Reise,

Kranz,

Heyder

et al. 2023

Molecules

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(1) Background: In the oral environment, sound enamel and dental restorative materials are immediately covered by a pellicle layer, which enables bacteria to attach. For the development of new materials with repellent surface functions, information on the formation and maturation of salivary pellicles is crucial. Therefore, the present in situ study aimed to investigate the proteomic profile of salivary pellicles formed on different dental composites. (2) Methods: Light-cured composite and bovine enamel samples (controls) were exposed to the oral cavity for 30, 90, and 120 min. All samples were subjected to optical and mechanical profilometry, as well as SEM surface evaluation. Acquired pellicles and unstimulated whole saliva samples were analyzed by SELDI–TOF–MS. The significance was determined by the generalized estimation equation and the post-hoc bonferroni adjustment. (3) Results: SEM revealed the formation of homogeneous pellicles on all test and control surfaces. Profilometry showed that composite surfaces tend to be of higher roughness compared to enamel. SELDI–TOF–MS detected up to 102 different proteins in the saliva samples and up to 46 proteins in the pellicle. Significant differences among 14 pellicle proteins were found between the composite materials and the controls. (4) Conclusions: Pellicle formation was material- and time-dependent. Proteins differed among the composites and to the control.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Salivary Pellicle Formed on Dental Composites Evaluated by Mass Spectrometry—An In Situ Study

Reise,

Kranz,

Heyder

et al. 2023

Molecules

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Oral microbiota and metabolites: key players in oral health and disorder, and microbiota-based therapies

Bostanghadiri,

Kouhzad,

Taki

et al. 2024

Front. Microbiol.

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The review aimed to investigate the diversity of oral microbiota and its influencing factors, as well as the association of oral microbiota with oral health and the possible effects of dysbiosis and oral disorder. The oral cavity harbors a substantial microbial burden, which is particularly notable compared to other organs within the human body. In usual situations, the microbiota exists in a state of equilibrium; however, when this balance is disturbed, a multitude of complications arise. Dental caries, a prevalent issue in the oral cavity, is primarily caused by the colonization and activity of bacteria, particularly streptococci. Furthermore, this environment also houses other pathogenic bacteria that are associated with the onset of gingival, periapical, and periodontal diseases, as well as oral cancer. Various strategies have been employed to prevent, control, and treat these disorders. Recently, techniques utilizing microbiota, like probiotics, microbiota transplantation, and the replacement of oral pathogens, have caught the eye. This extensive examination seeks to offer a general view of the oral microbiota and their metabolites concerning oral health and disease, and also the resilience of the microbiota, and the techniques used for the prevention, control, and treatment of disorders in this specific area.

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Proteomic profile of in situ acquired pellicle on tooth and restorative material surfaces

Cited by 2 publications

References 59 publications

Salivary Pellicle Formed on Dental Composites Evaluated by Mass Spectrometry—An In Situ Study

Salivary Pellicle Formed on Dental Composites Evaluated by Mass Spectrometry—An In Situ Study

Oral microbiota and metabolites: key players in oral health and disorder, and microbiota-based therapies

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