Proteomic profile of extracellular vesicles in maternal plasma of women with fetal death

Gallo, Dahiana; Fitzgerald, Wendy; Romero, Roberto; Gomez‐Lopez, Nardhy; Gudicha, Dereje W.; Than, Nándor Gábor; Bosco, Mariachiara; Chaiworapongsa, Tinnakorn; Jung, Eun-Jung; Meyyazhagan, Arun; Suksai, Manaphat; Gotsch, Francesca; Erez, Offer; Tarca, Adi L.; Margolis, Leonid

doi:10.1080/14767058.2023.2177529

Cited by 4 publications

(2 citation statements)

References 204 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have also adopted this criterion. [44][45][46] Therefore, the 90% confidence intervals (CIs) and p values <0.1 were chosen.…”

Section: Discussionmentioning

confidence: 99%

Childhood respiratory diseases related to indoor and outdoor extreme thermal environment (air temperature) in Shanghai, China

Hong,

Luo,

Zhu

et al. 2023

Indoor and Built Environment

View full text Add to dashboard Cite

Ambient air temperature could be the most significant factor impacting childhood respiratory health. This paper aims to analyze the relationship between air temperature and childhood respiratory diseases (RDs). Field measurements and meteorological parameters recorded from the weather website were conducted. Health outcomes regarding respiratory diseases amongst preschool children were reported. Multiple logistic regression analyses were conducted. Results from logistic regression analyses illustrate that extreme air temperature was significantly associated with a high prevalence of RDs amongst children. Indoor high temperature (>28°C) was positively correlated with the long duration of a common cold (≥2 weeks), and outdoor high temperature (>35°C) was positively related to the high prevalence of pneumonia. Indoor and outdoor low temperature (<18°C and <4°C, respectively) was a risk factor for rhinitis amongst children. The largest indoor air temperature difference (IATD) (the 4th quartile, >4°C) and outdoor air temperature difference (OATD) (the 4th quartile, >8°C) were positively related to the high prevalence of pneumonia and diagnosed asthma amongst children. Large differences between indoor and outdoor air temperatures and large indoor air temperature variations should be avoided to reduce the adverse effects on respiratory health, especially in extreme climates.

show abstract

“…Several studies have also adopted this criterion. [44][45][46] Therefore, the 90% confidence intervals (CIs) and p values <0.1 were chosen.…”

Section: Discussionmentioning

confidence: 99%

Childhood respiratory diseases related to indoor and outdoor extreme thermal environment (air temperature) in Shanghai, China

Hong,

Luo,

Zhu

et al. 2023

Indoor and Built Environment

View full text Add to dashboard Cite

show abstract

“…PE affects 2-8% of all pregnancies worldwide (7,29), leading to the deaths of more than 75,000 women and 500,000 fetuses annually (4), but as the symptoms can usually be detected only in the second half of gestation, early detection of the disease using appropriate biomarkers remains a challenge. Recently, several studies have proposed the role of non-coding RNAs (ncRNAs) in the etiology of PE (30)(31)(32)(33), and an emerging view is that these molecules could be transported in the circulation in membrane-encapsulated extracellular vesicles (EVs) and thus have diagnostic significance (34)(35)(36)(37)(38)(39)(40). Among the ncRNA groups, small RNAs of the RNA interference (RNAi) pathways are attractive targets for investigation because their functional roles have been more extensively elucidated as opposed to other classes, such as long ncRNAs or Y RNAs (31,41).…”

Section: Introductionmentioning

confidence: 99%

Exosomal small RNA profiling in first-trimester maternal blood explores early molecular pathways of preterm preeclampsia

Gál,

Fóthi,

Orosz

et al. 2024

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

IntroductionPreeclampsia (PE) is a severe obstetrical syndrome characterized by new-onset hypertension and proteinuria and it is often associated with fetal intrauterine growth restriction (IUGR). PE leads to long-term health complications, so early diagnosis would be crucial for timely prevention. There are multiple etiologies and subtypes of PE, and this heterogeneity has hindered accurate identification in the presymptomatic phase. Recent investigations have pointed to the potential role of small regulatory RNAs in PE, and these species, which travel in extracellular vesicles (EVs) in the circulation, have raised the possibility of non-invasive diagnostics. The aim of this study was to investigate the behavior of exosomal regulatory small RNAs in the most severe subtype of PE with IUGR.MethodsWe isolated exosomal EVs from first-trimester peripheral blood plasma samples of women who later developed preterm PE with IUGR (n=6) and gestational age-matched healthy controls (n=14). The small RNA content of EVs and their differential expression were determined by next-generation sequencing and further validated by quantitative real-time PCR. We also applied the rigorous exceRpt bioinformatics pipeline for small RNA identification, followed by target verification and Gene Ontology analysis.ResultsOverall, >2700 small RNAs were identified in all samples and, of interest, the majority belonged to the RNA interference (RNAi) pathways. Among the RNAi species, 16 differentially expressed microRNAs were up-regulated in PE, whereas up-regulated and down-regulated members were equally found among the six identified Piwi-associated RNAs. Gene ontology analysis of the predicted small RNA targets showed enrichment of genes in pathways related to immune processes involved in decidualization, placentation and embryonic development, indicating that dysregulation of the induced small RNAs is connected to the impairment of immune pathways in preeclampsia development. Finally, the subsequent validation experiments revealed that the hsa_piR_016658 piRNA is a promising biomarker candidate for preterm PE associated with IUGR.DiscussionOur rigorously designed study in a homogeneous group of patients unraveled small RNAs in circulating maternal exosomes that act on physiological pathways dysregulated in preterm PE with IUGR. Therefore, our small RNA hits are not only suitable biomarker candidates, but the revealed biological pathways may further inform us about the complex pathology of this severe PE subtype.

show abstract

Differences in Factors Associated With Preterm and Term Stillbirth: A Secondary Cohort Analysis of the DESiGN Trial

Winsloe,

Elhindi,

Vieira

et al. 2024

BJOG

View full text Add to dashboard Cite

ObjectiveTo identify whether maternal and pregnancy characteristics associated with stillbirth differ between preterm and term stillbirth.DesignSecondary cohort analysis of the DESiGN RCT.SettingThirteen UK maternity units.PopulationSingleton pregnant women and their babies.MethodsMultiple logistic regression was used to assess whether the 12 factors explored were associated with stillbirth. Interaction tests assessed for a difference in these associations between the preterm and term periods.Main Outcome MeasureStillbirth stratified by preterm (<37+0 weeks') and term (37+0–42+6 weeks') births.ResultsA total of 195 344 pregnancies were included. Six hundred and sixty‐seven were stillborn (3.4 per 1000 births), of which 431 (65%) were preterm. Significant interactions were observed for maternal age, ethnicity, IMD, BMI, parity, smoking, PAPP‐A, gestational hypertension, pre‐eclampsia and gestational diabetes but not for chronic hypertension and pre‐existing diabetes. Stronger associations with term stillbirth were observed in women with obesity compared to BMI 18.5–24.9 kg/m2 (BMI 30.0–34.9 kg/m2 term adjusted OR 2.1 [95% CI 1.4–3.0] vs. preterm aOR 1.1 [0.8–1.7]; BMI ≥ 35.0 kg/m2 term aOR 2.2 [1.4–3.4] vs. preterm aOR 1.5 [1.2–1.8]; p‐interaction < 0.01), nulliparity compared to parity 1 (term aOR 1.7 [1.1–2.7] vs. preterm aOR 1.2 [0.9–1.6]; p‐interaction < 0.01) and Asian ethnicity compared with White (p‐interaction < 0.01). A weaker or lack of association with term, compared to preterm, stillbirth was observed for older maternal age, smoking and pre‐eclampsia.ConclusionDifferences in association exist between mothers experiencing preterm and term stillbirth. These differences could contribute to design of timely surveillance and interventions to further mitigate the risk of stillbirth.

show abstract

Proteomic profile of extracellular vesicles in maternal plasma of women with fetal death

Cited by 4 publications

References 204 publications

Childhood respiratory diseases related to indoor and outdoor extreme thermal environment (air temperature) in Shanghai, China

Childhood respiratory diseases related to indoor and outdoor extreme thermal environment (air temperature) in Shanghai, China

Exosomal small RNA profiling in first-trimester maternal blood explores early molecular pathways of preterm preeclampsia

Differences in Factors Associated With Preterm and Term Stillbirth: A Secondary Cohort Analysis of the DESiGN Trial

Contact Info

Product

Resources

About