Proteomic profile of extracellular matrix from native and decellularized chorionic canine placenta

Matias, Gustavo de Sá Schiavo; Barreto, Rodrigo Egydio; Carreira, Ana Cláudia Oliveira; Nishiyama-Jr, Milton Yutaka; Fratini, Paula; Ferreira, Christina R.; Miglino, María Angélica

doi:10.1016/j.jprot.2022.104497

Cited by 8 publications

(4 citation statements)

References 56 publications

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“…The second abundant component in PL-dECM was the glycoproteins’ part, compromising fibrinogen, fibrillin-1 and periostin, which were equipped for promoting tissue regeneration as reported [ 73–76 ]. Consistent with the above findings, other proteome analyses of the decellularized placenta, decellularized tendon, decellularized matrix derived from small intestinal submucosa and decellularized dental pulp showed that they not only contained a substantial presence of ECM and adhesion-related proteins but also retained an abundance of tissue-specific proteins, which are crucial for tissue engineering [ 77–81 ]. Hence, it could be inferred that the PL-dECM contains numerous bioactive ingredients that exert positive cellular responses, warranting further discussion.…”

Section: Discussionsupporting

confidence: 65%

Periapical lesion-derived decellularized extracellular matrix as a potential solution for regenerative endodontics

Hu,

Jiang,

Deng

et al. 2024

Regenerative Biomaterials

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Pulp regeneration remains a crucial target in the preservation of natural dentition. Using decellularized extracellular matrix is an appropriate approach to mimic natural microenvironment and facilitate tissue regeneration. In this study, we attempted to obtain decellularized extracellular matrix from periapical lesions (PL-dECM) and evaluate its bioactive effects. The decellularization process yielded translucent and viscous PL-dECM, meeting the standard requirements for decellularization efficiency. Proteomic sequencing revealed that the PL-dECM retained essential extracellular matrix components and numerous bioactive factors. The PL-dECM conditioned medium could enhance the proliferation and migration ability of periapical lesion-derived stem cells (PLDSCs) in a dose-dependent manner. Culturing PLDSCs on PL-dECM slices improved odontogenic/angiogenic ability compared to the type I collagen group. In vivo, the PL-dECM demonstrated a sustained supportive effect on PLDSCs and promoted odontogenic/angiogenic differentiation. Both in vitro and in vivo studies demonstrated that PL-dECM served as an effective scaffold for pulp tissue engineering, providing valuable insights into PLDSCs differentiation. These findings pave avenues for the clinical application of dECM's in situ transplantation for regenerative endodontics.

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Section: Discussionsupporting

confidence: 65%

Periapical lesion-derived decellularized extracellular matrix as a potential solution for regenerative endodontics

Hu,

Jiang,

Deng

et al. 2024

Regenerative Biomaterials

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“…Em relação a recelularização dos biomateriais, tanto de células mesenquimais e de células mesenquimais em diferenciação para linhagem osteogênica, pode-se dizer que ocorreram de forma satisfatória. Fato este relacionado à preservação dos constituintes da matriz extracelular, mas também do arranjo os biomateriais (BARACHO TRINDADE HILL et al, 2020;BARRETO et al, 2022;LIU et al, 2022), fornecendo assim não apenas a aderência das células na superfície, mas também sua invasão pelos poros que antes eram habitados pelas células presentes nos cotilédones.…”

Section: Discussionunclassified

“…Em relação à placenta animais, pouco ainda se sabe, porém o uso da placenta bovina para produção de biomateriais é possível devido ao processo de descelularização (por perfusão) pouco agressivo desenvolvido por Barreto et al, 2018 que proporcionou a manutenção de componentes fibrosos, os quais são responsáveis pela estruturação da matriz extracelular (colágeno I e III) e flexibilidade (elastina); proteoglicanos, os quais atuam na regulação e adesão da matriz extracelular, além da capacidade de influenciar o metabolismo celular, devido a sua capacidade de retenção de fatores de crescimento; glicoproteínas multiadesivas (laminina e fibronectina), que estão presentes na membrana basal, ancorando as células ao colágeno tipo IV; e os glicosaminoglicanos, que devido a sua característica hidrofílica, atua como uma reserva de citocinas, hormônios e fatores de crescimento (BARRETO et al, 2023;DE SÁ SCHIAVO MATIAS et al, 2022). Entretanto, quando se pensa em descelularização em maior escala, a descelularização pode ser facilitada (ou abreviada) por imersão e agitação dos agentes descelularizantes químicos e/ou biológicos, do que quando realizada por perfusão (CRAPO; GILBERT; BADYLAK, 2011; KEANE; SWINEHART; .…”

Section: A Placenta Na Bioengenharia De Tecidosunclassified

>b<Produção de enxertos ósseos a partir da placenta bovina descelularizada>/b<

Cordeiro

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DedicatóriaÀ minha mãe, Maria Angela Smirnow Cordeiro e ao meu pai, Adilson Cordeiro, por me apoiarem e sempre me darem a estrutura necessária para que eu pudesse realizar meus sonhos. Eu amo vocês! À minha cachorra, Nanica, que me proporcionou várias lembranças, as quais levarei para toda a vida e, que, por diversos momentos da minha infância, foi a única amizade verdadeira que tive.Por fim, dedico este trabalho a todos os animais que serviram de estudos para a minha formação. AGRADECIMENTOSAos meus pais, Maria Angela Smirnow Cordeiro e Adilson Cordeiro, e à minha irmã, Paola Smirnow Cordeiro, por sempre me apoiarem durante esta jornada na pósgraduação. Aos meus melhores amigos, Rafael Rodrigues, Maurício Oliveira e Caio Victor, os quais conheci diretamente (Rafael e Maurício) e indiretamente (Caio) devido à pósgraduação. Agradeço imensamente pelo companheirismo, conselhos, conversas, risadas, intermináveis conversas de madrugada, em momentos bons e ruins, que, com certeza, fizeram a diferença na minha vida.

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“…It is composed of various types of laminins, collagens and nidogens which interact with large cell membrane receptors such as integrins, dystroglycans as well as smaller transmembrane receptors such as Fibronectin Leucine Rich transmembrane Proteins (FLRTs). Proteomics studies of the Basal Lamina has been limited and curtailed due to severe limitations which arise due to dynamic range of quantification of these extracellular components due to their large size (Calve et al, 2020;de Sá Schiavo Matias et al, 2022;Li et al, 2022;Pokhilko et al, 2021). The basement membrane is further subdivided into the basal lamina(BL) and the reticular lamina (RL).…”

Section: Basement Membranementioning

confidence: 99%

The role of extracellular matrix in zebrafish cardiac development and regeneration

Ramkumar¹

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Heart development is a dynamic process modulated by various extracellular and intracellular cues. Cardiac progenitors in vertebrates such as the zebrafish, migrate over to the midline after differentiation from the epiblast (Bakkers, 2011; Rosenthal & Harvey, 2010; Stainier et al., 1996; Trinh & Stainier, 2004). These progenitors form a cardiac disc at the midline which elongates into the linear heart tube. The differentiation and migration of cardiac precursors is modulated by signaling interactions between cardiac precursor cells and their extracellular environment known as the Extracellular Matrix (ECM). Studies have shown that Cell-ECM interactions play a crucial role in sculpting the heart during early morphogenic events (Davis CL, 1924; Männer & Yelbuz, 2019; Rosenthal & Harvey, 2010). One key factor to these processes is the presence of a specialized ECM known as the Basement Membrane (BM). Extracellular basement membrane proteins such as Fibronectin have been shown to modulate these very early migration processes of the cardiomyocyte progenitors (Trinh & Stainier, 2004). As the heart develops further, the linear heart tube is composed of myocardial cells with an inner endothelial cell lining separated by a layer of thick jelly like substance called the cardiac jelly (Barry A, 1948; Davis CL, 1924; Little et al., 1989). The cardiac jelly also called the cardiac basement membrane, has been shown to regulate distinct developmental events during cardiogenesis. This early CJ contains components of the basal lamina such as laminins, fibronectin, hyaluronan as well as non-fibrillar collagens such as Collagen IV (Little et al., 1989). In this study, I aimed to identify ECM molecules of the Basement Membrane in the heart and identify their role in the modulation of cardiac development and regeneration using the zebrafish as my model organism. I identified genes belonging to the Zebrafish Matrisome expressed during cardiac developmental and regeneration and performed CRISPR/Cas9 sgRNA mediated mutagenesis. I also developed overexpression tools for these genes. Agrinp168 mutants exhibited no obvious gross morphology defects during cardiac development and were adult viable. Adult mutants exhibited reduced cardiomyocyte proliferation, but no significant difference in cardiomyocyte dedifferentiation post cardiac cryoinjury. Decorin overexpression through mRNA injections led to increased myocardial wall thickness and DN dcn overexpression through mRNA injections led to loss of cardiac looping during early development. Mutants for Small Leucine Rich Proteoglycan (SLRP) prelp generated using CRISPR/Cas9 mutagenesis exhibited cardiovascular defects. Close observation of prelp mutant hearts revealed a reduced heart rate and impaired fractional shortening of the ventricle. prelp mutants exhibited an enlarged atrium at 48 hpf and 72 hpf as well as a reduced ventricle size at 72 hpf. Chamber size in the mutant hearts were enlarged irrespective of contractility of the heart. Mutants showed an increased number of Atrial cardiomyocytes, but no change in cell size. On the molecular level, extracellular Laminin localization was disrupted in prelp mutants along with an increase in thickness and volume of the cardiac HA in the CJ suggesting a potential compensatory role, or retention of immaturity of the cardiac jelly in the prelp mutants. Transcriptomics analysis on the prelp mutant hearts revealed downregulation of ECM organization and ECM-Receptor interaction processes in the mutants. Gene Ontology analysis on prelp mutants hearts transcriptome revealed increased MAPK signaling. Interestingly, genes related to degradation of cardiac HA and maturation of cardiac jelly were downregulated, and genes related to epithelial identity of cardiomyocytes were upregulated. Analysis of the mutant hearts at single cell resolution revealed increased number of mutants exhibiting rounded up cardiomyocytes and loss of apical Podocalyxin. Truncated forms of prelp were generated to identify domain specific roles for Prelp, and reintroduction of N-terminal truncated Prelp into the mutants rescued the basal lamina localization and cardiac jelly volume phenotypes. Myocardium specific re-establishment of prelp expression revealed a marked rescue of the mutant cardiovascular phenotype suggesting that tissue specific expression of prelp is not required so long as Prelp is secreted into the CJ. With these data, I’ve elucidated the role of ECM SLRPs in modulation of cardiac chamber morphogenesis process and regeneration of the heart.

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Proteomic profile of extracellular matrix from native and decellularized chorionic canine placenta

Cited by 8 publications

References 56 publications

Periapical lesion-derived decellularized extracellular matrix as a potential solution for regenerative endodontics

Periapical lesion-derived decellularized extracellular matrix as a potential solution for regenerative endodontics

>b<Produção de enxertos ósseos a partir da placenta bovina descelularizada>/b<

The role of extracellular matrix in zebrafish cardiac development and regeneration

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