Proteomic profile of cultured human endothelial cells after exposition to simulated microgravity

Каширина, Д. Н.; Kononikhin, Alexey; Ratushnyy, A. Yu.; Nikolaev, Evgeny N.; Ларина, И. М.; Buravkova, Ludmila

doi:10.1016/j.actaastro.2020.10.014

Cited by 2 publications

(2 citation statements)

References 32 publications

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“…This type of study was extended to human cells grown in microgravity [ 93 ] and protein extracts treated with CPLL before analysis. The results confirmed the previous conclusions with an increase in filamin-A, alpha-actinin and myosin light polypeptide 6.…”

Section: Other Medical Involvementsmentioning

confidence: 99%

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Boschetti¹,

Righetti

2023

IJMS

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The discovery of low- and very low-abundance proteins in medical applications is considered a key success factor in various important domains. To reach this category of proteins, it is essential to adopt procedures consisting of the selective enrichment of species that are present at extremely low concentrations. In the past few years pathways towards this objective have been proposed. In this review, a general landscape of the enrichment technology situation is made first with the presentation and the use of combinatorial peptide libraries. Then, a description of this peculiar technology for the identification of early-stage biomarkers for well-known pathologies with concrete examples is given. In another field of medical applications, the determination of host cell protein traces potentially present in recombinant therapeutic proteins, such as antibodies, is discussed along with their potentially deleterious effects on the health of patients on the one hand, and on the stability of these biodrugs on the other hand. Various additional applications of medical interest are disclosed for biological fluids investigations where the target proteins are present at very low concentrations (e.g., protein allergens).

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Section: Other Medical Involvementsmentioning

confidence: 99%

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Boschetti¹,

Righetti

2023

IJMS

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“…Indeed, it appeared that the effects of the absence of gravity are damages to the cytoskeleton by complex means of regulation involving Rho proteins. Additional studies involving CPLLs have been reported on the culture of human endothelial cells under microgravity [98]. The expression of proteins associated with cytoskeleton and fibers, such as filamin‐A, alpha‐actinin, and myosin light polypeptide 6, increased.…”

Section: Acceptance Of Cpll Technology Over Time and Major Driversmentioning

confidence: 99%

Combinatorial peptides: A library that continuously probes low‐abundance proteins

Boschetti¹,

Zilberstein

Righetti

2021

Electrophoresis

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After a decade of experimental applications, it is the objective of this review to make a point on combinatorial peptide ligand libraries dedicated to low‐abundance proteins from animals to plants and to microorganism proteomics. It is, thus, at the light of the recent technical developments and applications that we will examine the state of the art, its usage within the scientific community, and its openness to unexplored fields. The improvements of the methodology and its implementation in connection with analytical determinations of combinatorial peptide ligand library (CPLL)‐treated samples are extensively reviewed and commented upon. Relevant examples covering few critical aspects describe the performance of the technology. Finally, a reflection on the technological future is attempted in particular by involving new concepts adapted to the limited availability of certain biological samples.

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Proteomic profile of cultured human endothelial cells after exposition to simulated microgravity

Cited by 2 publications

References 32 publications

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Low-Abundance Protein Enrichment for Medical Applications: The Involvement of Combinatorial Peptide Library Technique

Combinatorial peptides: A library that continuously probes low‐abundance proteins

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