Proteomic Profile During Embryonic Development of Dengue Vector Aedes albopictus Mosquito

Abstract

Despite the potential impact of moisture on embryonation and egg eclosion of Aedes mosquitoes, little is known about its effect on protein synthesis during critical embryonic development as well as in the proteomic profiles. Thus, we quantify the protein concentration and proteomic profile during embryonic development of Ae. albopictus from far early of egg laying to egg eclosion in contact with sufficient moisture. It was observed that the concentration of protein started to decrease from the early hours (6th h) with progressing of embryonic development. There were more or less 13 bands observed in Coomassie blue staining of different embryonic stages within the range of 58 kDa and ~7 kDa by using 12% separating gel in 1D SDS-PAGE. Among them highly expressed bands on the position of 11-13 of lower molecular weight at around 7 kDa were found in all treatments. They may have controlling effects on egg hatching. Identification of these specific proteins can give an insight direction of effective vector control way.

Distinctions in proteomics among developmental stages of dengue vector (Aedes albopictus) mosquitoes

“…In our earlier communication, we focused on the association of moisture on the embryonic proteomic profile of Ae. albopictus (Saifur et al 2014) followed by visualized clarified embryo and spontaneous egg hatching with increasing moisture exposure (Saifur et al 2010). In connection with the previous, this work has indicated the projection of early proteomic cursors in the latter stages of life cycle up to the emergence as an adult that perform all activities in the vectorial life of the mentioned vector mosquito species.…”

“…albopictus mosquitoes for our study to avoid unwanted or extra proteins that fed adult mosquitoes get from different hosts with their blood meals (Rohani et al 2005). We found 7 more bands than the immature stage (Saifur et al 2014) within the range of MW ~60 to ~200 kDa. The bands with lower MW seen in the immature stage were reduced or fainted in the higher developmental stage.…”