Proteomic Patterns of Cultured Breast Cancer Cells and Epithelial Mammary Cells

Pucci‐Minafra, Ida; Fontana, Simona; Cancemi, Patrizia; Alaimo, Giuseppe; Minafra, Salvatore

doi:10.1111/j.1749-6632.2002.tb04103.x

Cited by 46 publications

(39 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The observed increase of the glycolytic enzyme expression is concordant with the increment of c-Myc expression, which is known to activate (directly or indirectly) a number of genes related to the glycolytic pathway [31,32]. The elevated levels of anaerobic metabolism, even in the presence of oxygen, known as the "Warburg effect," is a frequent clinical syndrome in oncologic patients, an observation recognized by several authors, including our group [17,25,26], as a frequent feature of neoplastic cells both in vivo and in vitro. It is also note worthy that the enzyme retinal dehydrogenase (ALIA1), which is a breast cancer stem cell marker, remains unchanged after fibroblast stimulation.…”

Section: Effect Of Coculture On Protein Expression Profiles Of 8701-bcsupporting

confidence: 49%

“…The protein identity was assessed by N-terminal sequencing and by MALDI-TOF. The identified proteins are grouped into 11 functional categories, according to our previously described criteria [17,25,26]: cytoskeleton and associated proteins, metabolic enzymes, molecular chaperones/heat shock proteins, membrane-associated proteins with multiple activities, calciumbinding proteins, proteins with binding function, biosynthesis and proliferation regulators, protein degradation, detoxification and redox proteins, proteins with extracellular activity, and signal transduction. The supplementary Table 1 reports the identified protein spots, displayed according to their functional category.…”

Section: Resultsmentioning

confidence: 99%

“…This allows the study of qualitative and quantitative variations between cells and tissues under given conditions [17,18]. The aim of this study was to investigate the effects of soluble mediators released by fibroblasts in vitro on breast cancer cells, using a proteomic-based approach.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiple Changes Induced by Fibroblasts on Breast Cancer Cells

Albanese¹,

Cara²,

Marabeti³

et al. 2009

Connective Tissue Research

Self Cite

View full text Add to dashboard Cite

It is now widely recognized that the cross-talk between cancer and stromal cells may play a crucial role in cancer progression. However, little is known about the complex underlying molecular mechanisms that occur within the tumor microenvironment. Fibroblasts are the major stromal cells with multiple roles, especially toward both the extracellular matrix and the neighboring cell population, including neoplastic cells. Consequently, proteomic analyses would provide a wider resource for a better understanding of the potential modulating effects exerted by fibroblasts on cancer cells. In this article we describe the effects of fibroblast stimulation on the breast cancer cell line (8701-BC) proteomics, using a trans-well coculture system. Our results clearly indicate that fibroblasts induce considerable proteomic modulations on 8701-BC, mainly in the cytoskeleton proteins and glycolytic enzymes. Additionally, fibroblast-conditioned medium increased neoplastic cell proliferation and invasion with a concurrent upregulation of the c-Myc oncogene. Collectively these results suggest that fibroblast stimulation may enhance the malignant potential of breast cancer cells in vitro.

show abstract

Section: Effect Of Coculture On Protein Expression Profiles Of 8701-bcsupporting

confidence: 49%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Multiple Changes Induced by Fibroblasts on Breast Cancer Cells

Albanese¹,

Cara²,

Marabeti³

et al. 2009

Connective Tissue Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…This event, originally interpreted as a consequence of a hypoxic environment within the tumor mass, appears plausible, but not exclusive to justify the increased protein expression of several key enzymes of the glycolysis, also under normoxic conditions "in vitro" [19], and in mammospheres [20]. Indeed, increased levels of gene expression of several glycolytic enzymes has been also detected in several cancer classes [21].…”

Section: The Glycolytic Enzymesmentioning

confidence: 99%

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Pucci‐Minafra¹,

Cara²,

Musso³

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

Abstract:The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The fragments were selected from patients with invasive ductal carcinoma of the breast, the most common and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers, useful for diagnostic and prognostic categorization of patients, in assessing postsurgical therapeutic regimes. The proteomic screening, by 2D-IPG and mass spectrometry, allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients, and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is generally supported by two concurrent pathways: the inhibition of apoptosis and the stimulation of cellular proliferation. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. To our knowledge this report on large-scales proteomics of breast cancer is currently a unique approach in the literature that offers the opportunity to evaluate the presence and recurrence of proteins to be used as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast.

show abstract

“…Gel calibration was carried out using an internal standard and the support of the ExPaSy molecular biology server, as described elsewhere [21]. Quantitative variations in the protein expression levels were calculated as the volume of the spots (i.e.…”

Section: Image Acquisition and Data Analysismentioning

confidence: 99%

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

Musso

Cara

Albanese

et al. 2013

Journal of Proteomics

Self Cite

View full text Add to dashboard Cite

Thyroid carcinomas account for a minority of all malignant tumours but, after those of the gonads, they represent the most common forms of endocrine cancers. They include several types, among which the papillary thyroid cancer (PTC) and the anaplastic thyroid cancer (ATC) are the best known. The two hystotypes display significant biological and clinical differences: PTC is a well differentiated form of tumour with a high incidence and a good prognosis, while the ATC is less frequent but represents one of the most aggressive endocrine tumours with morphological features of an undifferentiated type. To date, as far as we know, no conclusive studies, useful to design arrays of molecular markers, have been published illustrating the phenotypic and proteomic differences between these two tumours. The aim of this work was to perform a comparative analysis of two thyroid cancer cell lines, derived respectively from papillary (BCPAP) and anaplastic (8505C) thyroid carcinomas. The comparative analysis included cell behaviour assays and proteomic analysis by 2D-PAGE and mass spectrometry. IntroductionThe human thyroid gland is composed of a basic structural unit, the follicle, consisting of a monolayer of well polarized cells, the thyrocytes, responsible for the T3/T4 hormone secretion, and of other peripheral cells, the parafollicular C cells, responsible for the secretion of calcitonin. The presence within the follicle of stem cells, or remnants of embryonic cells, has been hypothesized as the target cells for tumour initiation. A thin extracellular matrix, which includes occasional fibroblasts and inflammatory cells, is peripheral to the follicle structure. Thyroid carcinomas account for 1-2% of all malignant tumours and, after those of the gonads, they represent the most common tumours of the endocrine system. The thyroid

show abstract

Proteomic Patterns of Cultured Breast Cancer Cells and Epithelial Mammary Cells

Cited by 46 publications

References 19 publications

Multiple Changes Induced by Fibroblasts on Breast Cancer Cells

Multiple Changes Induced by Fibroblasts on Breast Cancer Cells

Retrospective Proteomic Screening of 100 Breast Cancer Tissues

Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines

Contact Info

Product

Resources

About