Proteomic overview and perspectives of the radiation-induced bystander effects

Chevalier, François; Hamdi, Dounia Houria; Saintigny, Yannick; Lefaix, Jean-Louis

doi:10.1016/j.mrrev.2014.11.008

Cited by 23 publications

(39 citation statements)

References 73 publications

(113 reference statements)

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“…In addition to the DNA targeting, the IR can affect plasma membrane and subcellular organelles, and induce the activation of cell stress response-related genes and intracellular signaling pathways, triggering cell death [ 29 ]. Additionally, the irradiated cells may affect their non-irradiated neighbors through the bystander effect, or the transmission of the apoptotic signals to the surrounding unirradiated cells through a direct cellular contact or intercellular communication, which leads the unirradiated cells to exhibit similar biological effects to those of the irradiated cells [ 32 ]. Combined, these effects lead to the DNA damage, chromosomal instability, mutation and apoptosis in cancer cells, ultimately killing them (Fig.…”

Section: Introductionmentioning

confidence: 99%

Role of metabolism in cancer cell radioresistance and radiosensitization methods

Tang

Fang

et al. 2018

J Exp Clin Cancer Res

312

214

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BackgroundRadioresistance is a major factor leading to the failure of radiotherapy and poor prognosis in tumor patients. Following the application of radiotherapy, the activity of various metabolic pathways considerably changes, which may result in the development of resistance to radiation.Main bodyHere, we discussed the relationships between radioresistance and mitochondrial and glucose metabolic pathways, aiming to elucidate the interplay between the tumor cell metabolism and radiotherapy resistance. In this review, we additionally summarized the potential therapeutic targets in the metabolic pathways.Short conclusionThe aim of this review was to provide a theoretical basis and relevant references, which may lead to the improvement of the sensitivity of radiotherapy and prolong the survival of cancer patients.

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Section: Introductionmentioning

confidence: 99%

Role of metabolism in cancer cell radioresistance and radiosensitization methods

Tang

Fang

et al. 2018

J Exp Clin Cancer Res

312

214

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“…While hadrontherapy allows a better precision of the radiation towards the tumor, intercellular communication triggered by the irradiated damaged cells could occur, counter-balancing such physical accuracy of accelerated ions by a biological imprecision which may represent an important cause for radiation side-effects. Despite numerous studies on bystander effects, the mechanisms underlying this cellular response and their physiological role are not well understood and more studies are required to elucidate the real consequences of a bystander effect within and outside the irradiated area (Chevalier et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

Lepleux

Marie-Brasset

Temelie

et al. 2019

J. Cell Commun. Signal.

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While the dose-response relationship of radiation-induced bystander effect (RIBE) is controversial at low and high linear energy transfer (LET), mechanisms and effectors of cell-to-cell communication stay unclear and highly dependent of cell type. In the present study, we investigated the capacity of chondrocytes in responding to bystander factors released by chondrosarcoma cells irradiated at different doses (0.05 to 8 Gy) with X-rays and C-ions. Following a medium transfer protocol, cell survival, proliferation and DNA damages were quantified in bystander chondrocytes. The bystander factors secreted by chondrosarcoma cells were characterized. A significant and major RIBE response was observed in chondrocyte cells (T/C-28a2) receiving conditioned medium from chondrosarcoma cells (SW1353) irradiated with 0.1 Gy of X-rays and 0.05 Gy of C-ions, resulting in cell survivals of 36% and 62%, respectively. Micronuclei induction in bystander cells was observed from the same low doses. The cell survival results obtained by clonogenic assays were confirmed using impedancemetry. The bystander activity was vanished after a heat treatment or a dilution of the conditioned media. The cytokines which are well known as bystander factors, TNF-α and IL-6, were increased as a function of doses and LET according to an ELISA multiplex analysis. Together, the results demonstrate that irradiated chondrosarcoma cells can communicate stress factors to non-irradiated chondrocytes, inducing a wide and specific bystander response related to both doses and LET. Electronic supplementary material The online version of this article (10.1007/s12079-019-00515-9) contains supplementary material, which is available to authorized users.

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“…However, non-targeted effects of IRs have been previously suggested to play a role in radiation-resistance of CHS to low-LET radiations [ 50 ]. Further studies with 3DCM using innovative tools will help understand the radiation-resistance of CHS in clinics [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

In vitro engineering of human 3D chondrosarcoma: a preclinical model relevant for investigations of radiation quality impact

et al. 2015

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BackgroundThe benefit of better ballistic and higher efficiency of carbon ions for cancer treatment (hadron-therapy) is asserted since decades, especially for unresectable or resistant tumors like sarcomas. However, hadron-therapy with carbon ions stays underused and raises some concerns about potential side effects for patients. Chondrosarcoma is a cartilaginous tumor, chemo- and radiation-resistant, that lacks reference models for basic and pre-clinical studies in radiation-biology. Most studies about cellular effects of ionizing radiation, including hadrons, were performed under growth conditions dramatically different from human homeostasis. Tridimensional in vitro models are a fair alternative to animal models to approach tissue and tumors microenvironment.MethodsBy using a collagen matrix, standardized culture conditions, physiological oxygen tension and a well defined chondrosarcoma cell line, we developed a pertinent in vitro 3D model for hadron-biology studies. Low- and high-Linear Energy Transfer (LET) ionizing radiations from GANIL facilities of ~1 keV/μm and 103 ± 4 keV/μm were used respectively, at 2 Gy single dose. The impact of radiation quality on chondrosarcoma cells cultivated in 3D was analyzed on cell death, cell proliferation and DNA repair.ResultsA fair distribution of chondrosarcoma cells was observed in the whole 3D scaffold. Moreover, LET distribution in depth, for ions, was calculated and found acceptable for radiation-biology studies using this kind of scaffold. No difference in cell toxicity was observed between low- and high-LET radiations but a higher rate of proliferation was displayed following high-LET irradiation. Furthermore, 3D models presented a higher and longer induction of H2AX phosphorylation after 2 Gy of high-LET compared to low-LET radiations.ConclusionsThe presented results show the feasibility and usefulness of our 3D chondrosarcoma model in the study of the impact of radiation quality on cell fate. The observed changes in our tissue-like model after ionizing radiation exposure may explain some discrepancies between radiation-biology studies and clinical data.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-015-1590-5) contains supplementary material, which is available to authorized users.

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Proteomic overview and perspectives of the radiation-induced bystander effects

Cited by 23 publications

References 73 publications

Role of metabolism in cancer cell radioresistance and radiosensitization methods

Role of metabolism in cancer cell radioresistance and radiosensitization methods

Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

In vitro engineering of human 3D chondrosarcoma: a preclinical model relevant for investigations of radiation quality impact

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