Proteomic Investigation on Anopheles gambiae in Burkina Faso Related to Insecticide Pressures from Different Climatic Regions

Abstract

In Sub-Saharan Africa, An. gambiae sensu lato (s.l.) Giles 190, largely contributes to malaria transmission. Therefore, the authors carry out a proteomic analysis to compare its metabolic state, depending on different pesticide pressures by selecting areas with/without cotton crops. The proteomes data are available via ProteomeXchange with identifier PXD016300. From a total of 1.182 identified proteins, 648 are retained for further statistical analysis and are attributed to biological functions, the most important of which being energy metabolism (120 proteins) followed by translation-biogenesis (74), cytoskeleton (71), stress response (62), biosynthetic process (60), signalling (44), cellular respiration (38), cell redox homeostasis (25), DNA processing (17), pheromone binding (10), protein folding (9), RNA processing (9), other proteins (26) and unknown functions (83). In the Sudano-Sahelian region, 421 (91.3%) proteins are found in samples from areas both with and without cotton crops. By contrast, in the Sahelian region, only 271 (55.0%) are common to both crop areas, and 233 proteins are up-regulated from the cotton area. The focus is placed on proteins with putative roles in insecticide resistance, according to literature. This study provides the first whole-body proteomic characterisation of An. gambiae s.l. in Burkina Faso, as a framework to strengthen vector control strategies.

Fumarate mitigates disruption induced by fenpropathrin in the silkworm Bombyx mori (Lepidoptera): A metabolomics study

“…These results were consistent with a proteomics investigation of Anopheles gambiae in which the majority of proteins associated with pesticide pressure were related to energy metabolism (Azaque et al. , 2021). Therefore, it is reasonable to believe that the TCA cycle is the primary pathway with the greatest influence on fenpropathrin resistance.…”

Limited stress and tissue-specific transcriptional and translational activity of transposable elements in mosquitoes

“…For An. gambiae , datasets evaluated were: Brain tissue - PXD000630 (66), whole body - PXD016300 (67); for Ae. aegypti we used: head tissues - PXD022665 (68), semen - PXD010293 (69), salivary gland - PXD002468 (70); finally, the dataset chosen to An.…”