Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots

Zhou, Ying; Li, Huiying; Chen, Haoran; Yang, Xiaoqin; Yu, Tingting; Wang, Yushuang; Wang, Yujue; Jiang, Keting; Wang, Yan; Chen, Zhanyu; Cui, Xiyan

doi:10.3390/plants11091173

Cited by 13 publications

(5 citation statements)

References 81 publications

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“…Interestingly, in the roots, only the activity of SOD increased under mannitol treatment. In contrast to our study, Zhou et al (2022) observed in Glycine max (L.) Merr. roots treated with PEG the increasing activities of CAT and PX.…”

Section: Discussioncontrasting

confidence: 99%

The involvement of metallothioneins and stress markers in response to osmotic stress in Avena sativa L.

Konieczna

Mierek‐Adamska

Warchoł

et al. 2023

J Agronomy Crop Science

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Osmotic stress frequently caused by drought is the most threatening environmental stress that remarkably reduces crop yield. Oat (Avena sativa L.) is sensitive to water deficiency during growth. Plant metallothioneins (pMTs) show tremendous promise in enhancing general stress tolerance in plants. This study aimed to verify whether pMTs and elements of the antioxidant defence system protect oat against osmotic stress.Coding and genomic regions of A. sativa L. MTs belonging to three different types were cloned. To evaluate the role of MTs in osmotic stress, the expression of genes encoding AsMT1-3 was checked by qRT-PCR in the roots and shoots of oat plants growing in a hydroponic culture in the presence of polyethylene glycol (PEG 6000) and mannitol. The expression of AsMT1-3 changed in response to osmotic stress; however, the changes depended on the type of MT and the treatment. The amount of AsMT3 transcript was about five-fold and nine-fold higher in shoots and roots, respectively, in the presence of mannitol. To further analyse the response of oat to osmotic stress, the level of phenolic compounds, soluble sugars, abscisic acid, and the activity of antioxidant enzymes were tested using spectrophotometric and chromatographic methods. During osmotic stress, the content of phenols, soluble sugars, abscisic acid, and the activity of catalase and peroxidase in shoots increased. In roots treated with PEG 6000, the amount of phenolic compounds was higher than that in roots treated with mannitol. The activity of superoxide dismutase was about 5-fold higher in roots than in shoots. MTs are involved in plant response to osmotic stress. In the future, insight provided in this study will lead to application in agriculture either by using MTs as molecular markers for stress-resistant crop cultivars or by a generation of genetically modified crops overexpressing MTs.

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Section: Discussioncontrasting

confidence: 99%

The involvement of metallothioneins and stress markers in response to osmotic stress in Avena sativa L.

Konieczna

Mierek‐Adamska

Warchoł

et al. 2023

J Agronomy Crop Science

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“…Some sucrose synthase genes in our study were significantly downregulated in weakly drought-tolerant K55 leaves, whereas they were not significantly changed in K58 leaves. This is consistent with the findings obtained by Zhou et al in soybean roots [ 73 ]. Another study showed that drought stress increased the transcript level of GmSusy45 in soybean leaves [ 74 ].…”

Section: Discussionsupporting

confidence: 94%

Comparative transcriptome and coexpression network analysis reveals key pathways and hub candidate genes associated with sunflower (Helianthus annuus L.) drought tolerance

Shi,

Hou,

et al. 2024

BMC Plant Biol

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Background Drought severely limits sunflower production especially at the seedling stage. To investigate the response mechanism of sunflowers to drought stress, we utilized two genotypes of sunflower materials with different drought resistances as test materials. The physiological responses were investigated under well-watered (0 h) and drought-stressed conditions (24 h, 48 h, and 72 h). Results ANOVA revealed the greatest differences in physiological indices between 72 h of drought stress and 0 h of drought stress. Transcriptome analysis was performed after 72 h of drought stress. At 0 h, there were 7482 and 5627 differentially expressed genes (DEGs) in the leaves of K55 and K58, respectively, and 2150 and 2527 DEGs in the roots of K55 and K58, respectively. A total of 870 transcription factors (TFs) were identified among theDEGs, among which the high-abundance TF families included AP2/ERF, MYB, bHLH,and WRKY. Five modules were screened using weighted gene coexpressionnetwork analysis (WGCNA), three and two of which were positively and negatively, respectively, related to physiological traits. KEGG analysis revealedthat under drought stress, “photosynthesis”, “carotenoid biosynthesis”, “starch and sucrose metabolism”, “ribosome”, “carotenoid biosynthesis”, “starch and sucrose metabolism”, “protein phosphorylation” and “phytohormone signaling” are six important metabolic pathways involved in the response of sunflower to drought stress. Cytoscape software was used to visualize the three key modules, and the hub genes were screened. Finally, a total of 99 important candidate genes that may be associated with the drought response in sunflower plants were obtained, and the homology of these genes was compared with that in Arabidopsis thaliana. Conclusions Taken together, our findings could lead to a better understanding of drought tolerance in sunflowers and facilitate the selection of drought-tolerant sunflower varieties.

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“…Under drought stress, the antioxidant defense system in T. mongolicus was activated. It effectively cleared reactive oxygen species (ROS) in the plant and alleviated membrane lipid peroxidation damage, representing a drought response strategy [124]. Our study reported that the POD genes Chr_11G252, Chr_05G105, and Chr_01G2755 exhibited high homology (E-value < 1.0E-50) with AtPOD3, AtPOD39, and AtPOD64, respectively.…”

Section: Discussionmentioning

confidence: 92%

Chromosome-level genome assembly provides insights into the genome evolution and functional importance of the phenylpropanoid–flavonoid pathway in Thymus mongolicus

Dang,

Xu,

Zhang

et al. 2024

BMC Genomics

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Background Thymus mongolicus (family Lamiaceae) is a Thyme subshrub with strong aroma and remarkable environmental adaptability. Limited genomic information limits the use of this plant. Results Chromosome-level 605.2 Mb genome of T. mongolicus was generated, with 96.28% anchored to 12 pseudochromosomes. The repetitive sequences were dominant, accounting for 70.98%, and 32,593 protein-coding genes were predicted. Synteny analysis revealed that Lamiaceae species generally underwent two rounds of whole genome duplication; moreover, species-specific genome duplication was identified. A recent LTR retrotransposon burst and tandem duplication might play important roles in the formation of the Thymus genome. Using comparative genomic analysis, phylogenetic tree of seven Lamiaceae species was constructed, which revealed that Thyme plants evolved recently in the family. Under the phylogenetic framework, we performed functional enrichment analysis of the genes on nodes that contained the most gene duplication events (> 50% support) and of relevant significant expanded gene families. These genes were highly associated with environmental adaptation and biosynthesis of secondary metabolites. Combined transcriptome and metabolome analyses revealed that Peroxidases, Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferases, and 4-coumarate-CoA ligases genes were the essential regulators of the phenylpropanoid–flavonoid pathway. Their catalytic products (e.g., apigenin, naringenin chalcone, and several apigenin-related compounds) might be responsible for the environmental tolerance and aromatic properties of T. mongolicus. Conclusion This study enhanced the understanding of the genomic evolution of T. mongolicus, enabling further exploration of its unique traits and applications, and contributed to the understanding of Lamiaceae genomics and evolutionary biology.

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Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots

Cited by 13 publications

References 81 publications

The involvement of metallothioneins and stress markers in response to osmotic stress in Avena sativa L.

The involvement of metallothioneins and stress markers in response to osmotic stress in Avena sativa L.

Comparative transcriptome and coexpression network analysis reveals key pathways and hub candidate genes associated with sunflower (Helianthus annuus L.) drought tolerance

Chromosome-level genome assembly provides insights into the genome evolution and functional importance of the phenylpropanoid–flavonoid pathway in Thymus mongolicus

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