Proteomic investigation of human cystic echinococcosis in the liver

Longuespée, Rémi; Casadonte, Rita; Kriegsmann, Mark; Wandernoth, Petra; Lisenko, Katharina; Mazzucchelli, Gabriel; Becker, Michael; Kriegsmann, Jörg

doi:10.1016/j.molbiopara.2016.12.002

Cited by 17 publications

(12 citation statements)

References 33 publications

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“…Of these, the first three are considered specific amyloidosis biomarkers. We used LC–MS/MS‐based proteomics, adequate for the identification of amyloidosis‐associated proteins and the subsequent classification of amyloidosis subtypes . In the present context, deposits could be found in large quantities within different organs.…”

Section: Discussionmentioning

confidence: 99%

“…Sections of intestinal villi with massive amyloidosis were chosen for LMD‐based microproteomic analysis to characterize the type of amyloidosis. Characterization was performed using a recently developed method for proteomic analysis of small laser‐microdissected formalin‐fixed, paraffin‐embedded (FFPE) samples . The procedure for LMD‐based microproteomics has been described previously and uses in‐solution tryptic digestion of proteins directly from tissue pieces.…”

Section: Methodsmentioning

confidence: 99%

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Accelerated pre‐senile systemic amyloidosis in PACAP knockout mice – a protective role of PACAP in age‐related degenerative processes

Reglődi

Jungling

Longuespée

et al. 2018

The Journal of Pathology

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Dysregulation of neuropeptides may play an important role in aging‐induced impairments. Among them, pituitary adenylate cyclase‐activating polypeptide (PACAP) is a potent cytoprotective peptide that provides an endogenous control against a variety of tissue‐damaging stimuli. We hypothesized that the progressive decline of PACAP throughout life and the well‐known general cytoprotective effects of PACAP lead to age‐related pathophysiological changes in PACAP deficiency, supported by the increased vulnerability to various stressors of animals partially or totally lacking PACAP. Using young and aging CD1 PACAP knockout (KO) and wild type (WT) mice, we demonstrated pre‐senile amyloidosis in young PACAP KO animals and showed that senile amyloidosis appeared accelerated, more generalized, more severe, and affected more individuals. Histopathology showed age‐related systemic amyloidosis with mainly kidney, spleen, liver, skin, thyroid, intestinal, tracheal, and esophageal involvement. Mass spectrometry‐based proteomic analysis, reconfirmed with immunohistochemistry, revealed that apolipoprotein‐AIV was the main amyloid protein in the deposits together with several accompanying proteins. Although the local amyloidogenic protein expression was disturbed in KO animals, no difference was found in laboratory lipid parameters, suggesting a complex pathway leading to increased age‐related degeneration with amyloid deposits in the absence of PACAP. In spite of no marked inflammatory histological changes or blood test parameters, we detected a disturbed cytokine profile that possibly creates a pro‐inflammatory milieu favoring amyloid deposition. In summary, here we describe accelerated systemic senile amyloidosis in PACAP gene‐deficient mice, which might indicate an early aging phenomenon in this mouse strain. Thus, PACAP KO mice could serve as a model of accelerated aging with human relevance. © 2018 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Accelerated pre‐senile systemic amyloidosis in PACAP knockout mice – a protective role of PACAP in age‐related degenerative processes

Reglődi

Jungling

Longuespée

et al. 2018

The Journal of Pathology

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“…Por otra parte, se ha observado que al comparar el perfil proteómico del líquido hidatídico, existen diferencias en la expresión de la proteína y adaptación del metacestodo en su entorno huésped, según la localización de la lesión quística; así como también isoformas en las fracciones citosólica, nuclear, mitocondrial y microsomal (Alvite & Esteves, 2016;Zeghir-Bouteldja et al, 2017). Por ende, se puede señalar que existe evidencia orientativa de asociación de proteínas específicas a diversos tejidos, fenotipos celulares, estadios del parásito y procesos patobioquímicos, así como procesos inmunológicos en la EQ (Longuespée et al, 2017). Tabla I. Algunas proteínas parasitarias identificadas mediante espectrometría de masas en tándem, en líquido de quistes hidatídicos humanos por infección con E. granulosus.…”

Section: Métodos Utilizadosunclassified

Aspectos Generales del Perfil Proteómico del Echinococcus granulosus

2019

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MANTEROLA, C.; GARCÍA, N. & ROJAS, R. Aspectos generales del perfil proteómico del Echinococcus granulosus. Int. J. Morphol., 37(2): 773-779, 2019. RESUMEN: La información disponible referente a las características proteómicas del E. granulosus es escasa (no supera los 50 estudios publicados); y nos parece que la identificación proteómica, podría mejorar la comprensión de algunas características bioquímicas e inmunológicas de la Equinococosis Quística (EQ). De tal modo que el proteoma de E. granulosus aún no está bien descrito. Sólo existen reportes de algunas secuencias de proteínas. El objetivo de este manuscrito fue comentar algunos aspectos de la evidencia existente respecto de los estudios del perfil proteómico del E. granulosus. Se recomienda el estudio de al menos el quiste y su pared, el líquido hidatídico y la víscera del hospedero. Para ello, existen metodologías que han sido empleadas para estudiar las características proteómicas de la EQ. Entre ellas, destacan SDS-PAGE, electroforesis bidimensional combinada con Western Blot, inmunoanálisis, y espectrometría de masas mediante técnicas MALDI-TOF. Se han identificado una serie de proteínas en muestras de EQ. Algunas de ellas, asociadas a procesos inmunológicos, de gluconeogénesis, glucogenolisis y glucogénesis. Por otra parte, se ha documentado la liberación de exosomas al líquido hidatídico por parte de los protoescólex y la capa germinativa; estructuras en las que se han identificado factores de virulencia asociados con la supervivencia del quiste. No obstante lo anteriormente señalado, se requiere de múltiples estudios exhaustivos en la materia para comprender mejor la caracterización perfil proteómico del E. granulosus.

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“…The principle of “in situ” digestion is similar to the one applied in MALDI imaging. For this reason, this method can also be used for the parallel identification of compounds detected by MALDI imaging …”

Section: Four Key Workflows For Diagnostic Proteomic Analysesmentioning

confidence: 99%

“…In a recent study on breast cancer, we could show that the identification of more than 1400 proteins was possible on <3000 cells . Since then, LMD‐microproteomics has been successfully applied for biomarker discovery in cancer and for the investigation of proteomic changes in tissues infected by parasites . In a recent study, we demonstrated that it could be combined with MALDI imaging data to identify proteins related to ITH .…”

Section: Four Key Workflows For Diagnostic Proteomic Analysesmentioning

confidence: 99%

Proteomics in Pathology

Longuespée

Casadonte²,

Schwamborn

et al. 2018

Proteomics

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Proteomic approaches are of growing importance in the biologist's toolbox. It greatly benefited from past and recent advances in sampling, chemical processing, mass spectrometry (MS) instrumentation, and data processing. MS-based analysis of proteins is now in the process of being translated in pathology for objective diagnoses. In this viewpoint, we present the workflows that we think are the most promising for applications in pathology. We also comment what we think are prerequisites for a successful translational implementation.

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Proteomic investigation of human cystic echinococcosis in the liver

Cited by 17 publications

References 33 publications

Accelerated pre‐senile systemic amyloidosis in PACAP knockout mice – a protective role of PACAP in age‐related degenerative processes

Accelerated pre‐senile systemic amyloidosis in PACAP knockout mice – a protective role of PACAP in age‐related degenerative processes

Aspectos Generales del Perfil Proteómico del Echinococcus granulosus

Proteomics in Pathology

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